LOCUS       sequence-351365-        2334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ddGFP sensor of Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB,  Heo WD
  TITLE     Intensiometric biosensors visualize the activity of multiple small 
            GTPases in vivo.
  JOURNAL   Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3.
  PUBMED    30643148
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-14"; volume: "10(1):211. doi"; pages: " 
            10.1038/s41467-018-08217-3"
COMMENT     Sequence Label: pAAV-CAG-G-Rac1
FEATURES             Location/Qualifiers
     source          1..2334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             931..987
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1756..1803,1804..1806,1807..2334)
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     CDS             1756..2334
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
ORIGIN
        1 atggccacca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
       61 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      121 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      181 ttcatgtacg gctccgaggc gtacgtgagg caccccgccg acatccccga ttacaagaag
      241 ctgcccttcc ccgagggctt caagtgggag cgcgtgatga acttcgaaga cggcggtctg
      301 gtgaccgtta cccaggactc ctccctgcag gacggcacgc tgatctgcaa ggtgaagatg
      361 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      421 gcctccaccg agatgctgta ccccgaagac ggcgtgctga agggccatag ctatcaggcc
      481 ctgaagctga aggacggcgg ccactacctg gtggagttcg agaccatcta catggccaag
      541 aagcccgtgc aactgcccgg cgattactgt gtggacacca agctggacat cacctcccac
      601 aacgaggact acaccatcgt ggaacagtac gggcgctccg agggccgcca ccgtctgggc
      661 atggacgagc ggtacaagtt agggcatggt acaggtagca caggcagcgg tagctccggt
      721 accctgtaca agtcgtacaa gtccggactc agatctcgag gcagctccat tttacctgga
      781 gataaaacaa ataaaaagaa agagaaagag cggccagaga tttctctccc ttcagatttt
      841 gaacacacaa ttcatgtcgg ttttgatgct gtcacagggg agtttacggg aatgccagag
      901 cagtgggccc gcttgcttca gggaagcgga gctactaact tcagcctgct gaagcaggct
      961 ggagacgtgg aggagaaccc tggaccaatg gtgagcaaga gcgaggaggt catcaaagag
     1021 ttcatgcgct tcaaggtgcg cttggagggc tccatgaacg gccacgagtt cgagatcgag
     1081 ggcgagggcg agggccgccc ttacgagggc acccagaccg ccaagctgaa ggtgaccaag
     1141 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc tgatcatgta cggctccaag
     1201 atgtacgtga agcaccccgc cgacgtcccc gattacatga agctgtcctt ccccgagggc
     1261 ttcaagtggg agcgcgtgat gcacttcgag gacggcggtc tggtgaccgc gacacaggac
     1321 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
     1381 cccgacggcc ccgtaatgca gaagaagacc ttgggctggg attatgccac cgagcgcctg
     1441 taccccgaag aaggcgtgct gaagggcgag ctcctggggc gcctgaagct gaaggacggc
     1501 ggcctcaacc tggtggagtc caagaccatc tacatggcca agaagcccgt gcaactgccc
     1561 ggctactact tcgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     1621 gtggaacagt acgagcgctc cgagggccgc caccacctgg ggcatggtac aggtagcaca
     1681 ggcagcggta gctccggtac cctgtacaag ggcagtggtt ccggactcag atctcgagct
     1741 caagcttcga attccatgca ggccatcaag tgtgtggtgg tgggagacgg agctgtaggt
     1801 aaaaattgcc tactgatcag ttacacaacc aatgcatttc ctggagaata tatccctact
     1861 gtctttgaca attattctgc caatgttatg gtagatggaa aaccggtgaa tctgggctta
     1921 tgggatacag ctggacaaga agattatgac agattacgcc ccctatccta tccgcaaaca
     1981 gatgtgttct taatttgctt ttcccttgtg agtcctgcat catttgaaaa tgtccgtgca
     2041 aagtggtatc ctgaggtgcg gcaccactgt cccaacactc ccatcatcct agtgggaact
     2101 aaacttgatc ttagggatga taaagacacg atcgagaaac tgaaggagaa gaagctgact
     2161 cccatcacct atccgcaggg tctagccatg gctaaggaga ttggtgctgt aaaatacctg
     2221 gagtgctcgg cgctcacaca gcgaggcctc aagacagtgt ttgacgaagc gatccgagca
     2281 gtcctctgcc cgcctcccgt gaagaagagg aagagaaaat gcctgctgtt gtaa
//
