LOCUS       sequence-351366-        2361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ddGFP sensor of Hras.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB,  Heo WD
  TITLE     Intensiometric biosensors visualize the activity of multiple small 
            GTPases in vivo.
  JOURNAL   Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3.
  PUBMED    30643148
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-14"; volume: "10(1):211. doi"; pages: " 
            10.1038/s41467-018-08217-3"
COMMENT     Sequence Label: pAAV-CAG-G-Hras
FEATURES             Location/Qualifiers
     source          1..2361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1012..1068
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1792..2361
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 atggccacca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
       61 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      121 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      181 ttcatgtacg gctccgaggc gtacgtgagg caccccgccg acatccccga ttacaagaag
      241 ctgcccttcc ccgagggctt caagtgggag cgcgtgatga acttcgaaga cggcggtctg
      301 gtgaccgtta cccaggactc ctccctgcag gacggcacgc tgatctgcaa ggtgaagatg
      361 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      421 gcctccaccg agatgctgta ccccgaagac ggcgtgctga agggccatag ctatcaggcc
      481 ctgaagctga aggacggcgg ccactacctg gtggagttcg agaccatcta catggccaag
      541 aagcccgtgc aactgcccgg cgattactgt gtggacacca agctggacat cacctcccac
      601 aacgaggact acaccatcgt ggaacagtac gggcgctccg agggccgcca ccgtctgggc
      661 atggacgagc ggtacaagtt agggcatggt acaggtagca caggcagcgg tagctccggt
      721 accctgtaca agtccggact cagatctcga gccaccatgc cttctaagac aagcaacact
      781 atccgtgttt tcttgccgaa caagcaaaga acagtggtca atgtgcgaaa tggaatgagc
      841 ttgcatgact gccttatgaa agcactcaag gtgaggggcc tgcaaccaga gtgctgtgca
      901 gtgttcagac ttctccacga acacaaaggt aaaaaagcac gcttagattg gaatactgat
      961 gctgcgtctt tgattggaga agaacttcaa gtagatttcc tgggatccgg cgcaacaaac
     1021 ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat ggtgagcaag
     1081 agcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcttggaggg ctccatgaac
     1141 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cttacgaggg cacccagacc
     1201 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     1261 ctgatcatgt acggctccaa gatgtacgtg aagcaccccg ccgacgtccc cgattacatg
     1321 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgcacttcga ggacggcggt
     1381 ctggtgaccg cgacacagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1441 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac cttgggctgg
     1501 gattatgcca ccgagcgcct gtaccccgaa gaaggcgtgc tgaagggcga gctcctgggg
     1561 cgcctgaagc tgaaggacgg cggcctcaac ctggtggagt ccaagaccat ctacatggcc
     1621 aagaagcccg tgcaactgcc cggctactac ttcgtggaca ccaagctgga catcacctcc
     1681 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     1741 gggcatggta caggtagcac aggcagcggt agctccggta ccctgtacaa gatgacggaa
     1801 tataagctgg tggtggtggg cgccggcggt gtgggcaaga gtgcgctgac catccagctg
     1861 atccagaacc attttgtgga cgaatacgac cccactatag aggattccta ccggaagcag
     1921 gtggtcattg atggggagac gtgcctgttg gacatcctgg ataccgccgg ccaggaggag
     1981 tacagcgcca tgcgggacca gtacatgcgc accggggagg gcttcctgtg tgtgtttgcc
     2041 atcaacaaca ccaagtcttt tgaggacatc caccagtaca gggagcagat caaacgggtg
     2101 aaggactcgg atgacgtgcc catggtgctg gtggggaaca agtgtgacct ggctgcacgc
     2161 actgtggaat ctcggcaggc tcaggacctc gcccgaagct acggcatccc ctacatcgag
     2221 acctcggcca agacccggca gggagtggag gatgccttct acacgttggt gcgtgagatc
     2281 cggcagcaca agctgcggaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc
     2341 tgcaagtgtg tgctctcctg a
//
