LOCUS       sequence-351367-        2304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ddGFP sensor of Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Kim J , Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB,  Heo WD
  TITLE     Intensiometric biosensors visualize the activity of multiple small 
            GTPases in vivo.
  JOURNAL   Nat Commun. 2019 Jan 14;10(1):211. doi: 10.1038/s41467-018-08217-3.
  PUBMED    30643148
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-14"; volume: "10(1):211. doi"; pages: " 
            10.1038/s41467-018-08217-3"
COMMENT     Sequence Label: pAAV-CAG-G-Cdc42
FEATURES             Location/Qualifiers
     source          1..2304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             910..966
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atggccacca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
       61 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      121 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      181 ttcatgtacg gctccgaggc gtacgtgagg caccccgccg acatccccga ttacaagaag
      241 ctgcccttcc ccgagggctt caagtgggag cgcgtgatga acttcgaaga cggcggtctg
      301 gtgaccgtta cccaggactc ctccctgcag gacggcacgc tgatctgcaa ggtgaagatg
      361 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      421 gcctccaccg agatgctgta ccccgaagac ggcgtgctga agggccatag ctatcaggcc
      481 ctgaagctga aggacggcgg ccactacctg gtggagttcg agaccatcta catggccaag
      541 aagcccgtgc aactgcccgg cgattactgt gtggacacca agctggacat cacctcccac
      601 aacgaggact acaccatcgt ggaacagtac gggcgctccg agggccgcca ccgtctgggc
      661 atggacgagc ggtacaagtt agggcatggt acaggtagca caggcagcgg tagctccggt
      721 accctgtaca agggcagcgg cagctccatt ttacctggag ataaaacaaa taaaaagaaa
      781 gagaaagagc ggccagagat ttctctccct tcagattttg aacacacaat tcatgtcggt
      841 tttgatgctg tcacagggga gtttacggga atgccagagc agtgggcccg cttgcttcag
      901 ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
      961 ggaccaatgg tgagcaagag cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     1021 ttggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgccct
     1081 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     1141 tgggacatcc tgtcccccct gatcatgtac ggctccaaga tgtacgtgaa gcaccccgcc
     1201 gacgtccccg attacatgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1261 cacttcgagg acggcggtct ggtgaccgcg acacaggact cctccctgca ggacggcacg
     1321 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     1381 aagaagacct tgggctggga ttatgccacc gagcgcctgt accccgaaga aggcgtgctg
     1441 aagggcgagc tcctggggcg cctgaagctg aaggacggcg gcctcaacct ggtggagtcc
     1501 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactactt cgtggacacc
     1561 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     1621 gagggccgcc accacctggg gcatggtaca ggtagcacag gcagcggtag ctccggtacc
     1681 ctgtacaagt ccggactcag atctcgagct caagcttcga attcggcaat gcagacaatt
     1741 aagtgtgttg ttgtgggcga tggtgctgtt ggtaaaacat gtctcctgat atcctacaca
     1801 acaaacaaat ttccatcgga atatgtaccg actgtttttg acaactatgc agtcacagtt
     1861 atgattggtg gagaaccata tactcttgga ctttttgata ctgcagggca agaggattat
     1921 gacagattac gaccgctgag ttatccacaa acagatgtat ttctagtctg tttttcagtg
     1981 gtctctccat cttcatttga aaacgtgaaa gaaaagtggg tgcctgagat aactcaccac
     2041 tgtccaaaga ctcctttctt gcttgttggg actcaaattg atctcagaga tgacccctct
     2101 actattgaga aacttgccaa gaacaaacag aagcctatca ctccagagac tgctgaaaag
     2161 ctggcccgtg acctgaaggc tgtcaagtat gtggagtgtt ctgcacttac acagagaggt
     2221 ctgaagaatg tgtttgatga ggctatccta gctgccctcg agcctccgga aactcaaccc
     2281 aaaaggaagt gctgtatatt ctaa
//
