LOCUS       sequence-351373-        2779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of mScarlet red fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2779)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7A)-mScarlet
FEATURES             Location/Qualifiers
     source          1..2779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      69..78
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..770
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(208..226)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     476..495
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     rep_origin      complement(1022..1610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1102..1121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1781..2572,2573..2641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2404..2423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2642..2746)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atacgactca ctataaggac atttgcttct gacacaactg tgttcactag caacctcaaa
       61 cagaattcgc caccatggtg agcaagggcg aggcagtgat caaggagttc atgcggttca
      121 aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
      181 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
      241 ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcaccaagc
      301 accccgccga catccccgac tactataagc agtccttccc cgagggcttc aagtgggagc
      361 gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg
      421 acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct gacggccccg
      481 taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac cccgaggacg
      541 gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc cgctacctgg
      601 cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg
      661 tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg gaacagtacg
      721 aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagagctcg
      781 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttgctcgc tttcttgctg
      841 tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctactaaact gggggatatt
      901 atgaagggcc ttgagcatct ggattctgcc taataaaaaa catttatttt cattgcaaaa
      961 ccatgggagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1441 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1681 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1741 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1801 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1861 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1921 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1981 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2041 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2101 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2161 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2221 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2281 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2341 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2401 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2461 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2521 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2581 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2641 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2701 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2761 aagtgccacc tgacgtcta
//
