LOCUS       sequence-351374-        3602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of SOX9 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3602)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7A)-SOX9
FEATURES             Location/Qualifiers
     source          1..3602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1831..2419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1911..1930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2590..3381,3382..3450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3213..3232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3451..3555)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaggacattt gcttctgaca caactgtgtt cactagcaac ctcaaacaga attcgagctc
       61 ggtacccggg gatccgcgta tgaatctcct ggaccccttc atgaagatga ccgacgagca
      121 ggagaagggc ctgtccggcg cccccagccc caccatgtcc gaggactccg cgggctcgcc
      181 ctgcccgtcg ggctccggct cggacaccga gaacacgcgg ccccaggaga acacgttccc
      241 caagggcgag cccgatctga agaaggagag cgaggaggac aagttccccg tgtgcatccg
      301 cgaggcggtc agccaggtgc tcaaaggcta cgactggacg ctggtgccca tgccggtgcg
      361 cgtcaacggc tccagcaaga acaagccgca cgtcaagcgg cccatgaacg ccttcatggt
      421 gtgggcgcag gcggcgcgca ggaagctcgc ggaccagtac ccgcacttgc acaacgccga
      481 gctcagcaag acgctgggca agctctggag acttctgaac gagagcgaga agcggccctt
      541 cgtggaggag gcggagcggc tgcgcgtgca gcacaagaag gaccacccgg attacaagta
      601 ccagccgcgg cggaggaagt cggtgaagaa cgggcaggcg gaggcagagg aggccacgga
      661 gcagacgcac atctccccca acgccatctt caaggcgctg caggccgact cgccacactc
      721 ctcctccggc atgagcgagg tgcactcccc cggcgagcac tcggggcaat cccagggccc
      781 accgacccca cccaccaccc ccaaaaccga cgtgcagccg ggcaaggctg acctgaagcg
      841 agaggggcgc cccttgccag aggggggcag acagccccct atcgacttcc gcgacgtgga
      901 catcggcgag ctgagcagcg acgtcatctc caacatcgag accttcgatg tcaacgagtt
      961 tgaccagtac ctgccgccca acggccaccc gggggtgccg gccacgcacg gccaggtcac
     1021 ctacacgggc agctacggca tcagcagcac cgcggccacc ccggcgagcg cgggccacgt
     1081 gtggatgtcc aagcagcagg cgccgccgcc acccccgcag cagcccccac aggccccgcc
     1141 ggccccgcag gcgcccccgc agccgcaggc ggcgccccca cagcagccgg cggcaccccc
     1201 gcagcagcca caggcgcaca cgctgaccac gctgagcagc gagccgggcc agtcccagcg
     1261 aacgcacatc aagacggagc agctgagccc cagccactac agcgagcagc agcagcactc
     1321 gccccaacag atcgcctaca gccccttcaa cctcccacac tacagcccct cctacccgcc
     1381 catcacccgc tcacagtacg actacaccga ccaccagaac tccagctcct actacagcca
     1441 cgcggcaggc cagggcaccg gcctctactc caccttcacc tacatgaacc ccgctcagcg
     1501 ccccatgtac acccccatcg ccgacacctc tggggtccct tccatcccgc agacccacag
     1561 cccccagcac tgggaacaac ccgtctacac acagctcact cgaccttgag tcgacctgca
     1621 ggcatgcaag cttgctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc
     1681 taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct
     1741 aataaaaaac atttattttc attgcaaaac catgggagca aaaggccagc aaaaggccag
     1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2881 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3181 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3541 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaat acgactcact
     3601 at
//
