LOCUS       sequence-351406-        9596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding HMGN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: HMGN1_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          113..1129
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1053..1075
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1137..1156
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1245..1269
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1576..1600)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1620..1643
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1678..2227
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1822..1839)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2049..2068
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2230..2232,2233..2235,2236..2949)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2230..2232,2233..2235,2236..2946)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2275..2296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2536..2555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2883..2904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3026..3614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3079..3099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3497..3508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3617..3633)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3618..3634)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3823..4056
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4128..4249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4165..4184)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4219..4238
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4289..4424
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4444..4463)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4445..4463)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4486..4844)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4526..4547)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4738..4757
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4871..4975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4976..5044,5045..5836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5194..5213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6007..6595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6496..6515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6770..7073
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7075..7273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7228..7248
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7291..7471
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7518..7643
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8140..8373
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8558..8602
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8900..9017
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9115..9494
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
       61 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      121 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      181 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      241 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
      301 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
      361 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
      421 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
      481 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
      541 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
      601 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
      661 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
      721 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg
      781 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
      841 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
      901 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
      961 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     1021 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     1081 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1141 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcccctagc cgcggaatct
     1201 agactgccgg atcgaattgt cgacgaattc ggatctggcc aatcacaagt ttgtacaaaa
     1261 aagcaggcac catgcccaag aggaaggtca gctccgccga aggcgccgcc aaggaagagc
     1321 ccaagaggag atcggcgcgg ttgtcagcta aacctcctgc aaaagtggaa gcgaagccga
     1381 aaaaggcagc agcgaaggat aaatcttcag acaaaaaagt gcaaacaaaa gggaaaaggg
     1441 gagcaaaggg aaaacaggcc gaagtggcta accaagaaac taaagaagac ttacctgcgg
     1501 aaaacgggga aacgaagact gaggagagtc cagcctctga tgaagcagga gagaaagaag
     1561 ccaagtctga ttagaaccca gctttcttgt acaaagtggt gggaggaggt ggatctggcg
     1621 actacaagga cgacgatgac aagtgactcg agcgggatca attccgcccc ccccctaacg
     1681 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     1741 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     1801 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     1861 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     1921 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     1981 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     2041 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     2101 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     2161 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     2221 gataatacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2281 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2341 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2401 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2461 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2521 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2581 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2641 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2701 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2761 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2821 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2881 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2941 tacaagtaaa gcggccgcga ctctagagtc gacctgcagg catgcaagct tcaggtagcc
     3001 ggctaacgtt atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     3061 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3181 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3241 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3301 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3361 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3421 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3481 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3541 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3601 ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa
     3661 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     3721 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     3781 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3841 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3901 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3961 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4021 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     4081 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4141 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4201 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4261 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4321 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4381 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4441 tcgccctata gtgagtcgta ttagcatgtg agcaaaaggc cagcataaat caaaagaata
     4501 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4561 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4621 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4681 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4741 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4801 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     4861 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4921 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4981 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5041 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5101 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5161 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5221 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5281 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5341 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5401 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5461 ttgggaaccg aagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5521 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5581 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5641 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5701 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5761 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5821 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5881 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5941 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6001 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6061 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6661 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat
     6721 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6781 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6841 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     6901 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6961 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7021 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7081 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7141 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7201 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7261 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7321 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7381 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7441 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7501 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7561 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7621 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7681 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7741 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7801 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7861 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     7921 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     7981 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8041 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8101 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8161 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8221 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8281 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8341 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8401 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8461 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8521 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8581 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8641 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8701 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8761 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     8821 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     8881 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     8941 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9001 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttagtac
     9061 cgggcccgct ctagccctag atttaaatcg atgtacgggc cagatatacg cgttgacatt
     9121 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     9181 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     9241 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     9301 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     9361 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     9421 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     9481 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     9541 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatg
//
