LOCUS       sequence-351407-       10834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding Sufu.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: Sufu_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          133..1149
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1073..1095
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1157..1176
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1265..1289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2802..2826)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2846..2869
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2904..3453
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3048..3065)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3275..3294
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3456..3458,3459..3461,3462..4175)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3456..3458,3459..3461,3462..4172)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3501..3522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3762..3781)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4109..4130
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4252..4840
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4305..4325)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4723..4734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4843..4859)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4844..4860)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5049..5282
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5354..5475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5391..5410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5445..5464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5515..5650
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5670..5689)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5671..5689)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5712..6070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5752..5773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5964..5983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6097..6201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6202..6270,6271..7062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6420..6439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7233..7821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7722..7741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7996..8299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8301..8499
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8454..8474
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8517..8697
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8744..8869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9366..9599
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9784..9828
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10126..10243
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10341..10720
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
       61 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      121 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      181 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      241 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      301 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
      361 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
      421 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
      481 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
      541 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
      601 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
      661 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
      721 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
      781 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
      841 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
      901 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
      961 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1021 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1081 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1141 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1201 ttcccctagc cgcggaatct agactgccgg atcgaattgt cgacgaattc ggatctggcc
     1261 aatcacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tggcggagct
     1321 gcggcctagc gtcgcccccg gtcccgccgc gcccccggcc tctggcccta gtgcccctcc
     1381 ggcctttgct tcactctttc ccccgggact gcacgccatc tacggagagt gtcgccgcct
     1441 ctaccctgac cagccgaacc cgctccaggt taccgctatc gtcaagtact ggttgggtgg
     1501 tccggacccc ttggactatg ttagcatgta caggaacatg gggagtcctt ctgccaacat
     1561 ccctgagcac tggcactaca tcagctttgg cctgagtgat ctctatggtg acaacagagt
     1621 ccatgagttt acaggaacag acggaccaag tggatttggc tttgagttga cgtttcgtct
     1681 gaagagagaa actggggagt ctgccccacc aacatggcca gcagagctga tgcagggcct
     1741 agcccgatat gtcttccagt cagagaacac cttctgtagc ggggaccatg tgtcttggca
     1801 cagccctttg gataacagtg agtcaagaat tcagcacatg ctgctgacgg aggacccaca
     1861 gatgcagcct gtgcggacac cctttggggt agtgactttc ctccagattg ttggtgtctg
     1921 cactgaggag ttacattcag cccaacagtg gaacgggcag ggcatcctgg aactactacg
     1981 gacagtgccc attgctggcg gtccctggct gataactgac atgcggcggg gagaaaccat
     2041 atttgagatc gatccgcacc tgcaagagag agttgacaaa ggcattgaga cagacggttc
     2101 taacctgagc ggcgtcagtg ccaagtgtgc ctgggatgac ctcagccggc ctccggagga
     2161 tgaagaggat agccggagca tctgcctcgg cacacagcct cggaggctgt ctggcaaaga
     2221 cacagagcag atccgggaga ccctgaggcg gggactggag attaacagca aacctgtcct
     2281 tccaccaatc aattctcagc gacagaacgg cctcacccac gacagggctc cgagccgcaa
     2341 ggacagtttg ggcagcgaca gctccacggc catcatcccc cacgagctga tccgcacacg
     2401 gcagctcgag agcgtgcatc taaaatttaa ccaagagtcg ggagccctca tccctctctg
     2461 cctaaggggc agactcctac atggccggca cttcacctac aagagtatca caggcgacat
     2521 ggccatcacg tttgtgtcca cgggagtgga aggcgccttt gccactgagg aacacccgta
     2581 tgcagctcac ggaccctggt tacaaattct gttgacagaa gagtttgtag agaagatgtt
     2641 ggaggactta gaagatctaa cctctccaga ggaatttaaa cttcccaaag agtacagctg
     2701 gcctgagaag aaactcaaag tgtccattct ccccgacgtg gtgttcgaca gtccgctaca
     2761 ctaggtcgac ggtaccgcgg gcccgaaggg tgggcgcgcc gacccagctt tcttgtacaa
     2821 agtggtggga ggaggtggat ctggcgacta caaggacgac gatgacaagt gactcgagcg
     2881 ggatcaattc cgcccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     2941 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     3001 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     3061 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     3121 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     3181 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     3241 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     3301 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     3361 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     3421 ggacgtggtt ttcctttgaa aaacacgata ataccatggt gagcaagggc gaggagctgt
     3481 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3541 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3601 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     3661 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3721 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3781 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3841 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3901 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3961 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4021 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     4081 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4141 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agagtcgacc
     4201 tgcaggcatg caagcttcag gtagccggct aacgttatca agcttatcga taatcaacct
     4261 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4321 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4381 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4441 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4501 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4561 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4621 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4681 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4741 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4801 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     4861 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     4921 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     4981 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5041 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     5101 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5161 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5221 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5281 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5341 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5461 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5521 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5581 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5641 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattag catgtgagca
     5701 aaaggccagc ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     5761 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     5821 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     5881 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     5941 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     6001 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     6061 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6121 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6181 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6241 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6301 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6361 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6421 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6481 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6541 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6601 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6661 ggggatcatg taactcgcct tgatcgttgg gaaccgaagc tgaatgaagc cataccaaac
     6721 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6781 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6841 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6901 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6961 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7021 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7081 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7141 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7201 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7261 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7321 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7381 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7441 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7501 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7561 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7621 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7681 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7741 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7801 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7861 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7921 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     7981 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     8041 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     8101 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     8161 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     8221 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     8281 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     8341 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     8401 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     8461 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
     8521 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8581 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8641 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8701 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8761 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8821 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8881 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8941 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     9001 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9061 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9121 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     9181 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     9241 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9301 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9361 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9421 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9481 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9541 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9601 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9661 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9721 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9781 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9841 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     9901 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     9961 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    10021 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
    10081 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
    10141 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    10201 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
    10261 cagcagagat ccagtttggt tagtaccggg cccgctctag ccctagattt aaatcgatgt
    10321 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
    10381 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    10441 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    10501 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    10561 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
    10621 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
    10681 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
    10741 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
    10801 ttaattattt tgtgcagcga tgggggcggg gggg
//
