LOCUS       sequence-351433-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose inducible expression of 3xFLAG-PolyS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Chavez Lab
  TITLE     Multiplex screening identifies broad modifiers of ALS-associated 
            proteotoxicity
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAG426-GAL-3xFLAG-PolyS
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(341..365)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(398..414)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(399..415)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(433..451)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaagc tttcttcctc ttcctcttcc tcttcctctt cctcttcctc ttcctcttcc
      121 tcttcctctt cctcttcctc ttcctcttcc tcttcctctt cctcttcctc ttcctcttcc
      181 tcttcctctt cctcttcctc ttcctcttcc tcttcctctt cctcttcctc ttcctcttcc
      241 tcttcctctt cctcttcctc ttcctcttcc tcttcctctt cctcttcctc ttcctcttcc
      301 tcttcctctt cctcttcctc ttcctcttcc tcgagctcta acccagcttt cttgtacaaa
      361 gtggtgatgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgagtcat
      421 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
      481 ggaaggagtt ag
//
