LOCUS       sequence-351436-        2571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose inducible expression of SoxN-A22-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Chavez Lab
  TITLE     Multiplex screening identifies broad modifiers of ALS-associated 
            proteotoxicity
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAG426-GAL-SoxN-A22-EGFP
FEATURES             Location/Qualifiers
     source          1..2571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1750..1815
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      1846..1855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1852..1854,1855..1857,1858..2571)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1852..1854,1855..1857,1858..2568)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1897..1918)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2158..2177)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2505..2526
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgctgacca tggaatcgga tatgaaaggc agcctgctgc atgccaccat gccgccgcat
       61 cacacaagtg ccgcgttgca cggacatgcg gcatcgccgt actccgccct agctcccctt
      121 atgaacctgg gccagtcgca cctgacccac tcgcagctga gccatcacaa ccaccatcat
      181 caccacatga gcgcccatat cgcggccagc cagagtccca atccgctgag ctcgctgcag
      241 tcctcgatgg cgaacaccct caatggcagc caggtggggc agcagcagca gcaacagcag
      301 cagcagcaga gctccccgct gcacagctcc agcgaattga gtcccaccca gagcagcatc
      361 ggaagccatc acatgacatc gccggtcagc caccagcagc acacgcaaca gcagcagcac
      421 gggcagcagc agcatctggg cgcgggatct gcattgagca gcctaactgg aggatccagc
      481 aacaacaaca acaacagcgc cacggccaac aagaaccaac agcacgcgga tcgtgtgaag
      541 cggcctatga atgcgttcat ggtttggtca cggggccagc ggangaagat ggcctcggac
      601 aaccccaaga tgcacaactc ggagatctcg aagcgactgg gcgcccagtg gnaggacctc
      661 tcggaatcgg agaagcgacc gttcatcgac gaggccaagc gcctgagggc ggtgcacatg
      721 aaggagcatc cggactacaa gtaccggccg cgccgcaaga ctaaaacgct gaccaagacc
      781 aaggagaagt acccaatggg cggcctgatg cccggacaga cggtgggcgg cggagcaccg
      841 ggtgagccgg tgacgcccac gcgggtgcaa ggtcagcctg gccagaacca gtcgctaaac
      901 ggtagtggag gcagtgcagc agcggcagcg gcggcggcag cagcggcagc ggcagcagcg
      961 gcagcggcgg cggcagcagc gcaacaggcg cgccaggata tgtaccagat gaacgcgccc
     1021 aatggataca tgcccaatgg ctacatgatg cacgcagatc cggccggagc ggcagcctat
     1081 cagacctcct acatgggcca gcactacgcg gcgcagcggt acgacatggg acacatgtac
     1141 aacaacgggt atgcgatgta ccagacggtg tccggcggac agacatcgcc gtacggcagc
     1201 agtctccagc agcccggatc accgtcgccg tatggcggca gtagcttgca gcagcagccc
     1261 ggcagtccga ctccgtacgg cggaggtgga ggcggtggcg gtcaggtttc ctgccagagc
     1321 cacagcccca gcgactcgag catcaagtcc gaaccggtgt cgcccagtcc cagtgccata
     1381 gcgctcaaca acaacaacaa catcaacaac aatcacatca tgaagcggga atacagctct
     1441 gcagcggcgg cagcagcagc agcagcagcg gcagcagccg ccggcggcgg cgagctgaat
     1501 cacctgatga atatgtacca tctgccggat gagcagcgcc acctgctgca ctatcagact
     1561 gactcgcccg atctgcagca gcagcaccag tcgatgcaac agcagcagca gcacctcccg
     1621 cagcaacacc tttcgcagca gcaccaacag atcccgcagc aacaccatac catgcagcag
     1681 cagcagcagc aacaccacct gcagcatcag caatcgctga gggcgatggc gccgctggca
     1741 cacatgatgg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     1801 gatgacgatg acaaggcggt accgcgggcc cgggatccac cggtcgccac catggtgagc
     1861 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1921 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1981 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2041 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2101 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2161 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2221 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2281 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2341 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2401 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2461 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2521 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta a
//
