LOCUS       sequence-351440-       10999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding CHAF1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: CHAF1B_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          128..1144
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1068..1090
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1152..1171
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1260..1284
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2968..2992)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3012..3035
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3070..3619
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3214..3231)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3441..3460
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3622..3624,3625..3627,3628..4341)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3622..3624,3625..3627,3628..4338)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3667..3688)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3928..3947)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4275..4296
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4418..5006
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4471..4491)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4889..4900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5009..5025)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5010..5026)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5215..5448
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5520..5641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5557..5576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5611..5630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5681..5816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5836..5855)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5837..5855)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5878..6236)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5918..5939)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6130..6149
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6263..6367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6368..6436,6437..7228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6586..6605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7399..7987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7888..7907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8162..8465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8467..8665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8620..8640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8683..8863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8910..9035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9532..9765
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9950..9994
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10292..10409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10507..10886
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
       61 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      121 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      181 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      241 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      301 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      361 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      421 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
      481 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
      541 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
      601 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
      661 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
      721 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
      781 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
      841 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
      901 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
      961 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1021 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1081 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1141 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattccc
     1201 ctagccgcgg aatctagact gccggatcga attgtcgacg aattcggatc tggccaatca
     1261 caagtttgta caaaaaagca ggcaccatga aagtcatcac ttgtgaaata gcctggcaca
     1321 acaaggagcc cgtgtacagc ctggacttcc agcatgggac ggctgggagg atccacagac
     1381 tggcgtctgc cggcgtggac accaatgtca ggatctggaa ggtagaaaag ggaccagatg
     1441 gaaaagccat cgtggaattt ttgtccaatc ttgctcgtca taccaaagcc gtcaatgttg
     1501 tgcgtttttc tccaactggg gaaattttag catcgggagg agatgatgct gtcatcctat
     1561 tgtggaaggt gaatgataac aaggagccgg agcagatcgc ttttcaggat gaggacgagg
     1621 cccagctgaa caaggagaac tggacggttg tgaagactct gcggggccac ttagaagatg
     1681 tgtatgatat ttgctgggca actgatggga atttaatggc ttctgcctct gtggataaca
     1741 cagccatcat atgggatgtc agcaaaggac aaaagatatc aatttttaat gaacataaaa
     1801 gttatgtcca aggagtaacc tgggaccctt tgggtcaata tgttgctact ctgagctgtg
     1861 acagggtgct gcgagtatac agtatacaga agaagcgtgt ggctttcaat gtttcgaaga
     1921 tgctgtctgg aataggggct gaaggagagg caagaagcta ccggatgttt cacgacgaca
     1981 gcatgaagtc tttcttccgt agactgagtt tcactcccga cggatctttg cttctcacgc
     2041 cagctggatg tgtggaatct ggtgaaaatg taatgaatac cacttatgtt ttctccagga
     2101 agaatcttaa aaggcccatc gctcatcttc catgtcctgg aaaagccact cttgctgttc
     2161 gctgctgtcc ggtctacttt gaactgaggc cagtggtgga aacgggtgtg gagctgatga
     2221 gtctgcccta ccgcctggtg tttgctgtgg cctcggagga ttccgtgctt ctgtatgaca
     2281 cccagcagtc cttccctttt ggttacgtgt ctaatataca ttaccacacc ctcagtgaca
     2341 tttcatggtc cagcgatggt gccttcctgg ccatttcttc cacggacggt tactgctcat
     2401 ttgtgacatt tgagaaagat gaacttggaa ttcctttgaa agagaagcca gttttgaaca
     2461 tgagaactcc tgatacagca aagaaaacca agagtcagac acatcgaggg tcttcgccag
     2521 gacccagacc ggtagaggga acccctgcca gcagaaccca agaccccagc agccccggca
     2581 cgactccccc tcaggccaga caggccccag ccccaacagt catcagggac cctccctcca
     2641 tcactcctgc tgtcaaaagc cccttgccgg ggccttcgga ggagaagacc ctgcagccca
     2701 gtagtcaaaa cacaaaagcc cacccatccc ggagggtcac tctgaacaca ctgcaagcct
     2761 ggagcaagac aacaccccgg agaataaact taacaccctt aaagacggac actccaccaa
     2821 gttctgtacc aaccagtgtg atttccaccc cttctacaga agaaattcag tcagagacgc
     2881 ctggagacgc tcagggcagt cccccagagc taaagcggcc cagactcgat gaaaacaaag
     2941 gaggcacgga aagtctggac ccttagaacc cagctttctt gtacaaagtg gtgggaggag
     3001 gtggatctgg cgactacaag gacgacgatg acaagtgact cgagcgggat caattccgcc
     3061 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     3121 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     3181 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     3241 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     3301 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     3361 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     3421 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     3481 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     3541 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     3601 tttgaaaaac acgataatac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3661 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3721 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3781 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3841 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3901 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3961 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4021 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4081 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4141 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4201 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4261 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4321 atggacgagc tgtacaagta aagcggccgc gactctagag tcgacctgca ggcatgcaag
     4381 cttcaggtag ccggctaacg ttatcaagct tatcgataat caacctctgg attacaaaat
     4441 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4501 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4561 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4621 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4681 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4741 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4801 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4861 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4921 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4981 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa
     5041 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     5101 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     5161 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5221 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5281 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5341 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5401 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5461 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     5521 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5581 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5641 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     5701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5821 gacgtaccca attcgcccta tagtgagtcg tattagcatg tgagcaaaag gccagcataa
     5881 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     5941 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     6001 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     6061 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     6121 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     6181 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     6241 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6301 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6361 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6421 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6481 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6541 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6601 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6661 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6721 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6781 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6841 tcgccttgat cgttgggaac cgaagctgaa tgaagccata ccaaacgacg agcgtgacac
     6901 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6961 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7021 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7081 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7141 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7201 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7261 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7321 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7381 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7441 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7501 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7561 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7621 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7681 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7741 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7801 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7861 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7921 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7981 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8041 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     8101 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     8161 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     8221 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     8281 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     8341 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     8401 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     8461 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     8521 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     8581 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     8641 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     8701 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8761 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8821 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8881 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8941 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     9001 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     9061 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     9121 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     9181 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     9241 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     9301 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     9361 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9421 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9481 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9541 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9601 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9661 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9721 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9781 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9841 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9901 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9961 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    10021 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    10081 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    10141 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    10201 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
    10261 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    10321 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10381 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
    10441 tttggttagt accgggcccg ctctagccct agatttaaat cgatgtacgg gccagatata
    10501 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
    10561 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    10621 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    10681 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    10741 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
    10801 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
    10861 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
    10921 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
    10981 cagcgatggg ggcgggggg
//
