LOCUS       sequence-351466-       12461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of  of DAF-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH4723
FEATURES             Location/Qualifiers
     source          1..12461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    98..119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(134..139,140..157,158..164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    172..188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     177..199
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     196..212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     196..212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9581..9598)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9581..9597)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9590..9612)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9810..10265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9897..9916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10107..10128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10282..10301)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10401..10423
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10461..10479)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10547..10651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10652..10720,10721..11512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10870..10889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11683..12271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12172..12191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12425..12442
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
       61 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      121 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      181 gataacaatt tcacacagga aacagctatg acatgattac gaattcgaat ggccatggga
      241 cgtcgacgca tagcccgcag tcacttgaaa gcacatttaa gatggaatca aatggtaaga
      301 aagctcgtgt aatcaggaga ttttatccgg ggtatgccga ggaatatatg gtgcactcgc
      361 attagctggc attttttgta attatcggat ttctaccttt ttcttaatct agtttgttat
      421 cattgtccat catttacaac caaaaatttg cgtcatttac aaccaaaaat tgatctgaca
      481 attatcataa ttcggttttt attttcagaa gaaacgctcc gatgtgatcc tatagtgaat
      541 atacttttcc atcctctctg aaataaacaa ttatgaagaa tgcatcaatt tgcattcgaa
      601 ataactgccg tattttgtct attggtatgg ctgcagtacc atttttcttc gcgtctttgc
      661 gcagccttcg ggcgctacca ataaggctaa gccggggtaa gttatttcag aattctaatc
      721 aactaatcta agtctcatac atcattgtca agtgacgcat ttttttaatt tctatgttaa
      781 ctcgacttta atattctttt ctggtttcac cagaaaaatg ctggactgaa aaattgaatt
      841 tcccgcgatt tgggaaaaac cgcagtttaa gcttttggca tgaatacagt gaacacagga
      901 acaaaaaatt gacttgttat tttgagttta tattaaatgt taattttgca tctcatattt
      961 tcacaaaact tgtttgcttg aaaaatgtgc aaataacaag tgaacataca tcaaggttta
     1021 tgcagatcag cactgatgca actgatagca aaactgataa gggtcaaaat ttcagtggcc
     1081 agcacaaaca catacacatt cgtagttcgt tttgtgtttc gtgtatttag catttatttt
     1141 tcaattccgg caatttgccg atttgccgga aattttgatt tttggcaaat tgccagtttg
     1201 ccgtttgccg aacatcaatt tgccgaaatt ttttagaggg atctttataa gacggaaaca
     1261 cttaaaattg tgcttttttg gaattttttt caatgtttca atagaattag cttgcattta
     1321 aaattagatg taggaacgtt tataggacgc ttacaatttt gccgattaaa atcgaagttc
     1381 tgaacttttc aaaaaatgtg caaaattttt tttgacaaat ttgacaaatc ggccggtttg
     1441 tcgattgtcc ggaatttttc agtttcggcg atttaccggt ttgccggaaa acatcgtttg
     1501 ccacccacac acacataaag agacgaaaca acacaccatc ttcaaaaaat ttgtgaatct
     1561 tcatttccac gtggtttgca gggtttccca tccaccccac tctatctctc caagttgaat
     1621 tttggcaaaa ttgaccacac accgatcaaa aactattgtg tgtgtgtgta tttgtgtctt
     1681 caataattcc tgtctgtgtc tcgcactatc tattgactac aagaacgtga tcgcagctct
     1741 acgaaacctt tactttattt tagaaatatt ttttcatgct attagcaaaa aaaaagtttt
     1801 tttttgtcta agtacactta aaattttcca aaatttttaa gatttttcaa aatgtgcgat
     1861 caaagatctg acaaaatgcc aaaattttga agaggaaatt tagtcggctt ccaaaattat
     1921 aagtggcaga aattgagcaa ttgtcacttt ttgacagtaa atagaaaatt ttttgaaaaa
     1981 ttttgaaacg ctttattttg ttacttggtc attttggcac cataggagta gtttttaaca
     2041 ttttccccac tggcgctact tcattttaaa cttttccaaa gattttaatt tttaatttta
     2101 aaaaatttca tgtaattcgt ctgcgtctta cagtttcagg cttcaaaaat ctattacata
     2161 tcttatcttt gaattcaggg atcccccggg atgaatattg tcagatgtcg gagacgacac
     2221 aaaattttgg aaaatttgga agaagagaat ctcggcccga gctgctcgtc gacgacttca
     2281 acaaccgctg ccaccgaagc tctcggaaca accactgagg atatgaggct taagcagcag
     2341 cgaagctcgt cgcgtgccac ggagcacgat attgtcgacg gcaatcacca cgacgacgag
     2401 cacatcacaa tgagacggct tcgacttgtc aaaaattcgc ggacgcggcg tagaacgacg
     2461 cccgattcaa gtatggactg ctatgaggaa aacccgccat cacaaaaaac ttcaataaat
     2521 tattcttgga tttctaaaaa gtcatcaatg acgtcattaa tgcttttact gctattcgct
     2581 tttgtacagc cgtgtgcctc aatagtcgaa aaacgatgcg gcccaatcga tattcgaaat
     2641 aggccgtggg atattaagcc gcaatggtcg aaacttggtg atccgaacga aaaagatttg
     2701 gctggtcaga gaatggtcaa ctgcacagtg gtggaaggtt cgctgacaat ctcatttgta
     2761 ctgaaacaca agacaaaagc acaagaagaa atgcatcgaa gtctacagcc aagatattcc
     2821 caagacgaat ttatcacttt tccgcatcta cgtgaaatta ctggaactct gctcgttttt
     2881 gagactgaag gattagtgga tttgcgtaaa attttcccaa atcttcgtgt aattggaggc
     2941 cgttcgctga ttcaacacta tgcgctgata atttatcgaa atccggattt ggagatcggt
     3001 cttgacaagc tttccgtaat tcgaaatggt ggtgtacgga taatcgataa tcgaaaactg
     3061 tgctacacga aaacgattga ttggaaacat ttgatcactt cttccatcaa cgatgttgtc
     3121 gttgataatg ctgccgagta cgctgtcact gagactggat tgatgtgccc acgtggagct
     3181 tgcgaagagg ataaaggcga atcaaagtgt cattatttgg aggaaaagaa tcaggaacaa
     3241 ggtgtcgaaa gagttcagag ttgttggtcg aacaccactt gccaaaagtc ttgtgcttat
     3301 gatcgtcttc ttccaacgaa agaaatcgga ccgggatgtg atgcgaacgg cgatcgatgt
     3361 cacgatcaat gcgtgggcgg ttgtgagcgt gtgaatgatg cgacagcatg ccacgcgtgc
     3421 aagaatgtct atcacaaggg aaagtgtatc gaaaagtgtg atgctcacct gtaccttctc
     3481 cttcaacgtc gttgtgtgac ccgtgagcag tgtctgcagc tgaatccggt gctctcgaac
     3541 aaaacagtgc ctatcaaggc gacggcaggc ctttgctcgg ataaatgtcc cgatggttat
     3601 caaatcaacc cggatgatca tcgagaatgc cgaaaatgcg ttggcaagtg tgagattgtg
     3661 tgcgagatca atcacgtcat tgatacgttt ccgaaggcac aggcgatcag gctatgcaat
     3721 attattgacg gaaatctgac gatcgagatt cgcggaaaac aggattcggg aatggcgtcc
     3781 gagttgaagg atatatttgc gaacattcac acgatcaccg gctacctgtt ggtacgtcaa
     3841 tcgtcaccgt ttatctcgtt gaacatgttc cggaatttac gacgtattga ggcaaagtca
     3901 ctgttcagaa atctatatgc tatcacagtt tttgaaaatc cgaatttaaa aaagctattc
     3961 gattcaacga cggatttgac gcttgatcgt ggaactgtgt caattgccaa taacaagatg
     4021 ttatgcttca agtatatcaa gcagctaatg tcaaagttaa atataccact cgatccgata
     4081 gatcaatcag aagggacaaa tggtgagaag gcaatctgtg aggatatggc aatcaacgtg
     4141 agcatcacag cggtcaacgc ggactcggtc ttctttagtt ggccctcatt caacattacc
     4201 gatatagatc agcgaaagtt tctcggctac gagctcttct tcaaagaagt cccacgaatc
     4261 gatgagaaca tgacgatcga agaggatcga agtgcgtgtg tcgattcgtg gcagagtgtc
     4321 ttcaaacagt actacgagac gtcgaacggt gaaccgaccc cggacatttt tatggatatt
     4381 ggaccgcgcg agcgaattcg gccgaatacg ctctacgcgt actatgtggc gacgcagatg
     4441 gtgttgcatg ccggtgcgaa gaacggtgta tcgaagattg gttttgtgag gacgagctac
     4501 tatacgcctg atcctccgac gttggcacta gcgcaagtcg attcggacgc tattcatatt
     4561 acgtgggaag cgccgctcca accgaacgga gacctcacgc attacacaat tatgtggcgt
     4621 gagaatgaag tgagcccgta cgaggaagcc gaaaagtttt gtacagatgc aagcaccccc
     4681 gcaaatcgac aacacacgaa agatccgaaa gagacgattg tagccgataa gccagtcgat
     4741 attccgtcat cacgtaccgt agctccgaca cttttgacta tgatgggtca cgaagatcag
     4801 cagaaaacgt gcgctgcaac gcccggttgt tgttcgtgtt cggctatcga agaatcatcg
     4861 gaacagaaca agaagaagcg accggatccg atgtcggcga tcgaatcatc tgcatttgag
     4921 aataagctgt tggatgaggt tttaatgccg agagacacga tgcgagtgag acgatcaatt
     4981 gaagacgcga atcgagtcag tgaagagttg gaaaaagctg aaaatttggg aaaagctcca
     5041 aaaactctcg gtggaaagaa gccgctgatc catatttcga agaagaagcc gtcgagcagc
     5101 agcaccacat ccacaccggc tccaacgatc gcatcaatgt atgccttaac aaggaaaccg
     5161 actacggtgc cgggaacaag gattcggctc tacgagatct acgaaccttt acccggaagc
     5221 tgggcgatta atgtatcagc tctggcattg gataatagtt atgtgatacg aaatttgaag
     5281 cattacacac tttatgcgat ttctctatcc gcgtgccaaa acatgacagt acccggagca
     5341 tcttgctcaa tatcccatcg tgcgggagca ttgaaacgaa caaaacacat cacagacatt
     5401 gataaagtgt tgaatgaaac aattgaatgg agatttatga ataatagtca acaagtcaac
     5461 gtgacgtggg atccaccgac tgaagtgaat ggtggaatat tcggttatgt tgtaaagctt
     5521 aagtcaaaag tcgatggatc aattgttatg acgagatgtg tcggtgcgaa gagaggatat
     5581 tcaacacgga atcagggtgt cctattccag aatttggccg atggacgtta ttttgtctca
     5641 gtaacggcga cctctgtaca cggcgctgga ccggaagccg aatcctccga cccaatcgtc
     5701 gtcatgacgc caggcttctt cactgtggaa atcattctcg gcatgcttct cgtctttttg
     5761 attttaatgt caattgccgg ttgtataatc tactactaca ttcaagtacg ctacggcaaa
     5821 aaagtgaaag ctctatctga ctttatgcaa ttgaatcccg aatattgtgt ggacaataag
     5881 tacaatgcag acgattggga gctacggcag gatgatgttg tgctcggaca acagtgtgga
     5941 gagggatcat tcggaaaagt gtacctcgga actggaaata atgttgtttc tctgatgggt
     6001 gatcgtttcg gaccgtgtgc tattaagatt aatgtagatg atccagcgtc gactgagaat
     6061 ctcaactatc tcatggaagc taatattatg aagaacttta agactaactt tatcgtcaaa
     6121 ctgtacggag ttatctctac tgtacaacca gcgatggttg tgatggaaat gatggatctt
     6181 ggaaatctcc gtgactatct ccgatcgaaa cgcgaagacg aagtgttcaa tgagacggac
     6241 tgcaactttt tcgacataat cccgagggat aaattccatg agtgggccgc acagatttgt
     6301 gatggtatgg cgtacctgga gtcgctcaag ttttgccatc gagatctcgc cgcacgtaat
     6361 tgcatgataa atcgggatga gactgtcaag attggagatt tcggaatggc tcgtgatcta
     6421 ttctatcatg actattataa gccatcgggc aagcgtatga tgcctgttcg atggatgtca
     6481 cccgagtcgt tgaaagacgg aaagtttgac tcgaaatctg atgtttggag cttcggagtt
     6541 gttctctatg aaatggttac actcggtgct cagccatata ttggtttgag taatgatgag
     6601 gtgttgaatt atattggaat ggcccggaag gttatcaaga agcccgaatg ttgtgaaaac
     6661 tattggtata aggtgatgaa aatgtgctgg agatactcac ctcgggatcg tccgacgttc
     6721 ctccagctcg ttcatcttct agcagctgaa gcttcaccag aattccgaga tttatcattt
     6781 gtcctaaccg ataatcaaat gatccttgac gattcagaag cactggatct tgatgatatt
     6841 gatgatactg atatgaatga tcaggttgtc gaggtggcac cggatgttga gaacgtcgag
     6901 gttcagagtg attcggaacg tcggaatacg gattcaatac cgttgaaaca gtttaagacg
     6961 atccctccga tcaatgcgac gacgagtcat tcgacaatat cgattgatga gacaccgatg
     7021 aaagcgaagc agcgagaagg atcgctggat gaggagtacg cattgatgaa tcatagtgga
     7081 ggtccgagtg atgcggaagt tcggacgtat gctggtgatg gagattatgt ggagagagat
     7141 gttcgagaga atgatgtgcc aacgcgacga aatactggtg catcaacatc aagttacaca
     7201 ggtggtggtc catattgcct aacaaatcgt ggtggttcaa atgaacgagg agccggtttc
     7261 ggtgaagcag tacgattaac tgatggtgtt ggaagtggac atttaaatga tgatgattat
     7321 gttgaaaaag agatatcatc catggatacg cgccggagca cgggcgcctc gagctcttcc
     7381 tacggtgttc cacagacgaa ttggagtgga aatcgtggtg ccacgtatta tacgagtaaa
     7441 gctcaacagg cagcaactgc agcagcagca gcagcagcag ctctccaaca gcaacaaaat
     7501 ggtggtcgag gcgatcgatt aactcaacta cccggaactg gacatttaca atcgacacgt
     7561 ggtggacaag atggagatta tattgaaact gaaccgaaaa attatagaaa taatggatct
     7621 ccatcggtac tggatttttt tttttggaga aattagccgg aaaattcgat ttttttcgct
     7681 gaaaatctga aaaaatttgg ctaaaaattg ccctgaaaca cgtttttttc ggaattttca
     7741 tataaaaatg gacattttcg taaaaaattg gctatgtgtt gtgaattttt tctgactttt
     7801 aaaggccttt gaactaggaa tattaaaatt ttattgaaaa ttattgaaaa gtgcaaattt
     7861 ttattttttt taggtgaaaa aagcttagat ttaggcgaat tttctgaaat tttatagtga
     7921 atttggttct caggatatta gccatttttt cccgattttt tggtaaaaat ttgttaaaac
     7981 ccgcttcaaa tgactgtagt tttatacaaa aataactaaa aattcgattt ttcaattatt
     8041 cagctcgaaa aagcccaaca aaaatattta aaatttgaag aaaaaagcta aaaatgagta
     8101 aaaatgactg gtttttttcg ctttttctat ttgaataaat ttctgaaaaa tgacgaaaat
     8161 ctgaaaaaat taccccggac attgggaaat ttcgaaaaaa tcttgaaaat agtctgaaat
     8221 ttttaaaatt tacagatttt cacaaaaaag ggaaatttcg catttcctga aattgcccaa
     8281 aaattttttt tttcattttt ttaaaaattc tggaaattta tttttggctg tttcaaattt
     8341 ttaaaaaaag aaaaatccca aaaattggtg aaaactccat taaaattctc cagaaaattc
     8401 aaattttcca tgaaaaattg ttttaaaagc atgaaaatct gccgattttt gtctaaaatt
     8461 tttaaaaata tttcaaaaat ttcagaaaaa acaggaaaag cgaatattac ataaataaaa
     8521 aatctggaaa ttctagaaac tgaaaatttt ttaatttttc cgctcaaaaa ttctaatatt
     8581 cgatgaaaat gagcccgaaa attcgagtaa atttccaaaa aaaatttttt tttttttcaa
     8641 acacaaatat taatttttat taatttttca gcgaaacggc aacagccgtg acattttcaa
     8701 cggacgttcg gctttcggtg aaaatgagca tctaatcgag gataatgagc atccacttgt
     8761 ggtaccatgg tattgatatc tgagctccgc atcggccgct gtcatcagat cgccatctcg
     8821 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     8881 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     8941 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     9001 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     9061 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     9121 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     9181 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     9241 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     9301 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     9361 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     9421 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     9481 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     9541 tctactagtg gccacgtggg ccgtgcacct taagcttggc actggccgtc gttttacaac
     9601 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9661 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9721 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     9781 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     9841 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9901 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9961 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    10021 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    10081 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    10141 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    10201 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
    10261 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
    10321 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    10381 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    10441 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    10501 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10561 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10621 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10681 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10741 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10801 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10861 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    10921 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    10981 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    11041 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    11101 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    11161 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    11221 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    11281 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    11341 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    11401 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    11461 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    11521 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11581 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11641 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11701 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11761 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    11821 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    11881 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    11941 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    12001 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    12061 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    12121 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    12181 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    12241 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    12301 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    12361 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    12421 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca a
//
