LOCUS       sequence-351467-       10368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1 INS-18::GFP unc-54 3' UTR C.elegans pan-neural expression of
            INS-18 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH1498
FEATURES             Location/Qualifiers
     source          1..10368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..44
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    161..182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(197..202,203..220,221..227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    235..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     240..262
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     259..275
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     259..275
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      5884..5893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5890..5892,5893..5895,5896..6609)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5890..5892,5893..5895,5896..6606)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5935..5956)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6196..6215)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6543..6564
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6634..6658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6684..6725
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7551..7568)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7551..7567)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7560..7582)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7780..8235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7867..7886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8077..8098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8252..8271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8371..8393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8431..8449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8517..8621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8622..8690,8691..9482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8840..8859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9653..10241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10142..10161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
       61 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      121 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      181 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      241 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattcg aatggccatg
      301 ggacgtcgac gcttgcatgc ctgcagcgtt tccgataccc ccttatatca gcacacattc
      361 agtcatcata ccacaaaatg ttgtgatagt gataggcaaa actaccgtat ttcctctatt
      421 aatcttgcat gcaagactaa ttttcgatta acccgtaggg gtgcaagact aatagagact
      481 gcaagactat tagaggctga aatactaatt ttcgtatgct caataatttt ggaaattggc
      541 ctattttttg tagaaacttg ataccgttta aacaaggaaa aatacacact ttttaatatt
      601 tcatcaataa tttgaacgat tttgtgattt taagttcaat ttgcccaaaa aaagacaatt
      661 tttctgacgt taccgcaaag taatgctggt caggcaaaaa gagcggtgca aaaatataag
      721 agactgcaat actaatagag gaaatacggt aattgaattt tagtgaaact tgcgacagtt
      781 ttcctcattt ttttgttatt ccggcatacg agtgtggcat acgagtgagg tcatcttttg
      841 tttcttccgt ttcttcatcg cttttgaaaa aaaatgttga agaaaacttc gtagccgata
      901 gccggataat gtttggcacg gcgttccaac atatatcatt ggaaattttt taagattttt
      961 gcgaaaaatc acattcttca cgatgagaac acgttattga aggaatataa atcaagaata
     1021 acatatagtt atatttctct attactttaa cgttaaatat gagcaaattt gagcattttg
     1081 attgcgatga aaagcagaat cgagtcaact gaaatccgtt caaaaaccta agctcggttc
     1141 gcccgaaagt cgatttttcc aatagccgaa cagcactgct tctttatttt ccaggtattt
     1201 ttggggtgta agaattatat cttctttgtt tgggtattta cgtttccgaa ttccctttct
     1261 tgatctgtcg ctatttttca gaaaccagaa cagtttatcc tttttcatga gaacctgaat
     1321 agctcaaaaa cccgtttaat ttcttttcat catctcattt gaaactttcc tagtcttctt
     1381 aatgatttcc aggctcctcc tacatttgta tctcaagatg catacacttt atttccgctc
     1441 caattcgaat catctacttc tcttttttct tcaactttct acttttttcc attcttttct
     1501 ttcaaatgtt cgacgtcttc aagtgactgc tcttcattct cctcattttt ctcgaatttc
     1561 attcttgtct cttttttgct caaaaatgga aaatgaaagt gacgtgagat ttggacggcg
     1621 ggacacgggg gcagtagaag cagcaaaaag gagagaaaga ggacacaaat aagaagaacg
     1681 aattcaaaaa taagcggaga ggagctattt ccgtcaattc tacctcccca atcttcatca
     1741 attcggctca attgaatgac gtcacaggga gataaaccgt ttggatgagc gccgtccatc
     1801 actgacgcca tcccgtttgg gacaagaaaa gagaaaaaag agcacaaagt ttttggtgac
     1861 ggatcttgtc aatcatatga aagttgttct gattgattgt cagttttttt cctacttttt
     1921 tggattctat ccacttctga acttttgaca agtttcaaac tttctgaatc attttctatg
     1981 cattttcctg gaattctttt tatgtaaaat atgaaataga atgtttttga attcaagttc
     2041 tgcttttttc ttcttttctg ttctgttcgg gcttgggtat gcttttttta aaaattattt
     2101 tgcacatcga ccaataagtg cgcaacttat aaaattaatt tatttttgtt aatttttgaa
     2161 atacttgtat tgctttaagt gatctgacct cgccctgagc tttccacgta gttatcaaat
     2221 acaaatcctc caagggtaac gtacctatat tactgatctt tataataact ttatcacctg
     2281 tccagttcca gaggaattct gttaagctta taggcacaga aggagtcatt tctgctggtt
     2341 tttaatgatc caaatcttta tttcaagtaa aaaactgaac acttgcgaat aaaactatca
     2401 gattaaccat tcaccaaaaa tgtgtttgaa tctaaaactt tctcagtatt ccaaatatag
     2461 aaataaataa ccacgacatt gctaaaatct gtctgaattg tgtactcctt accgtgaagt
     2521 aataatggat ataatgaatc gtttgaaatg aatgatcagc acatttttgg tgaaagatca
     2581 caaataagga ataagcgtcg gaaaataaaa cgattatttc ggatcaaaaa tttgttgaag
     2641 atcatataca ctcgagacca agattattct agacaatttt caaattggct tctttgtttg
     2701 caaatcttca taataatctg tgaagtttgg aaatttgaat ttttaatctt ttttcataga
     2761 ttatagtttt tatttctttg caaaactata ttaaaaaccg atgcattgtt ttagggaaat
     2821 taatgagcct tttgttcaac actaaaaaca ataaaattaa aattttggct tcatcatttg
     2881 acctttttta agttcgaaaa cttttctcgt atttctgaac cgccaatttt ttcacacatc
     2941 tctagacttt tggtgcccgt tccagaaagt taagtaattg ctattctaag aaagttctca
     3001 acattgtttt ttagttctga ttgaattctg atgttccagg aatatatttt aaatttaata
     3061 ttctttgcac tatttctata ctaactaaat aataaatagt ctaagtatgt tcaatgagaa
     3121 caaaaaagct ctatctatat ttttctatcc tatttcattt atatcctttt cattttgaac
     3181 tcaccagctt ctcttcttct tcttgtccat atacttctta aaagtctcga acttctctct
     3241 ctcactctcc atcaatttct ttatggctac cgcttgcgct ctgccgcttc cgaagaaaga
     3301 aggcgttggc agagctctca attctatttt ttctcgccgt aggctatcga tttctcagct
     3361 tctcttctct cttcctcact ccaccaccac cacttcgggc ttcttctttt cttcattttc
     3421 gtcttcttct tcatcctctt cttttttttt cagttccccc tcactcctcc ccttatatgc
     3481 gcgtgcgaga ggggtgcaaa agcagcgcgc atccgagaat tgaaacgaga aaacggagac
     3541 gcagcagcag ttcgtcctca gaaaaatagc cagtaaaaag agaaaaagat agagagagac
     3601 ctatcgcatt ttattttcaa attgcaattc ctgcaattca tgtgtgcgtg tgcccatttt
     3661 caattcttcc cgttattttt cagaccatta ccaacgttct ttctagatct tgagacaatc
     3721 ttccttctgc tcaatcgttc gtcgtgaaga cggcatcgac gacgacgacg atcagcagta
     3781 aaggatcctt atggtccacc gacttttcat cgtccttatt gcaattattc ttgtcgcaaa
     3841 atcaactgca atctcacttc aacaagctgg tttgttttca tgtttgtaat acaaattttt
     3901 ttaacttttg gaaaaaatat gtatcagata gcaaacttga taataacttt acaaaatcta
     3961 aatagattca cttgggaaag ttcgacacca atccctgtgg gaggaaaaat tttaatttcc
     4021 cagcacgttc tctgtgacat tttcgattca ttgtcctaac aaaatttgat aagtttgtga
     4081 gaatttcaca tagttcgtaa gcacttggtg gagattgcat aactgaattg ctcacaacat
     4141 aaaattagca aaataaatga aaacagggaa atattatatt tgcaagttta atttctacaa
     4201 gaactggttt caaacatcca aactctttcg gggaaggctt gtcccgattt tgaaatttta
     4261 tctcattctg gtgtcctaga aacaattgaa aatatgtcaa gcttctaggt agctggaaac
     4321 tcctagcttt agcttccgat attggcacaa gtacaaattc caaaaaccaa aaaatccaac
     4381 tttaacgaat ttttctcatt attagtcaaa aagagagaaa gggagagtag ttataggtcc
     4441 ttaaaaagtc cacattttat atctcatttt cccattgtct cctttctttc ttttgcattc
     4501 attcctagtt tgacttcaaa tcctaaatcc caaaaagctc agctttctct tggtttgcca
     4561 agtcgactgc attctcttag ctgagatgtt ttatgaatac tagataacca ccaaacaaac
     4621 tatattctta gaagaatgag ggatatcggg ctttatgaca atcataaaac atcgacttgt
     4681 gacgtggcat agggaaaaga aaaaagagac aagacgaacg gctcaaataa ataaggaaac
     4741 agggaaataa cattttttcg ggagagctgt ttagcgtgat taatatattg ctatcatatt
     4801 attcctatca tatttacatt aaaagaaaca cattttggca attggcaatt atttaattct
     4861 ttttttgaag aaacattgct ttcaaatgga gcactattag gagagatgtg aaaatgaaat
     4921 ttaaatgaag ttttaaatta tcatacttcc ctcatcaata cttgcatttc aatattaaac
     4981 tttggcaact catatcttga ttgaccaaaa aagtgggcaa atgtttataa ttaaaaataa
     5041 gtaaaaaaca ttggaagatt aaaaacttta gaaacttttt gagaaaaaaa aactgaaaaa
     5101 tgtcacaaga aaaagtgctt cctgtcctcg gaaaaaactg ataaatgatt caaaaagtgt
     5161 ttcaaacaat ggtaaccatg gataattgag catcttcttt cagacggacg catgaaaatg
     5221 tgcccaccag gtggttcaac attcacaatg gcatggtcaa tgtcgtgttc gatgcgcagg
     5281 agaaaacgag atgttggacg atatttcggt aatttttttg ggtagagaac tagaacatta
     5341 gagaacatta gaaacctttg gccgaatatg tgagaaccag caaaatttag aaagaatttt
     5401 ctaatatttc cttcgaatca aagtgggaga caattatttt gcagttgcaa tatctctaaa
     5461 atcaagtgtt aaaaaataca taaacctgtc tccctcctgt ctgacaaaga attttgtttg
     5521 ttaattgaaa aatgtttaaa agacgtacaa aaattgcctg aaagttgtca attgaaaaaa
     5581 ccttcacgga cctcttcgac cactcgcaat ttccatgtaa tttttaacag atcaacacaa
     5641 aatatttaaa tattttagag ttacacaaat tttaattttt atataataac tttgggtcaa
     5701 gataccacaa aaaatccttt tgtatagttt ccaacattat taatttccaa aatctttttt
     5761 tttgcagaaa aacgtgctct gatcgcccca tcaatccgtc aacttcaaac aatttgctgt
     5821 caagttggtt gcaacgtgga agatcttctt gcctactgtg ccccaattgg ggatccaccg
     5881 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     5941 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     6001 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     6061 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     6121 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     6181 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6241 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6301 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     6361 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     6421 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6481 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     6541 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6601 tacaagtaaa gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttg
     6661 atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
     6721 ctacgcgtac cggttagtaa tgagtttatc tgagctccgc atcggccgct gtcatcagat
     6781 cgccatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     6841 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     6901 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     6961 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     7021 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     7081 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     7141 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     7201 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     7261 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
     7321 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
     7381 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
     7441 tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa
     7501 cataactgtt tctactagtg gccacgtggg ccgtgcacct taagcttggc actggccgtc
     7561 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7621 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7681 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     7741 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     7801 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7861 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7921 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7981 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8041 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8101 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     8161 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     8221 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8281 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8341 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8401 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8461 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8521 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8581 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8641 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8701 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8761 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8821 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8881 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8941 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9001 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9061 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9121 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9181 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9241 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9301 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9361 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9421 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9481 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9781 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10021 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10261 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10321 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccg
//
