LOCUS       sequence-351468-       12628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding GLI1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12628)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 12628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: GLI1_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..12628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          112..1128
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1052..1074
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1136..1155
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1244..1268
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4593..4617)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4637..4660
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4695..5244
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4839..4856)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5066..5085
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5247..5249,5250..5252,5253..5966)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5247..5249,5250..5252,5253..5963)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5292..5313)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5553..5572)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5900..5921
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6043..6631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6096..6116)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6514..6525)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6634..6650)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6635..6651)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6840..7073
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7145..7266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7182..7201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7236..7255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7306..7441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7461..7480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7462..7480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(7503..7861)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7543..7564)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7755..7774
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7888..7992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7993..8061,8062..8853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8211..8230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9024..9612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9513..9532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9787..10090
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10092..10290
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10245..10265
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10308..10488
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10535..10660
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11157..11390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11575..11619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11917..12034
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        12132..12511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
       61 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
      121 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
      181 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
      241 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
      301 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
      361 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
      421 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
      481 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
      541 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
      601 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
      661 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
      721 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
      781 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
      841 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
      901 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
      961 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     1021 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1081 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1141 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcccctagcc gcggaatcta
     1201 gactgccgga tcgaattgtc gacgaattcg gatctggcca atcacaagtt tgtacaaaaa
     1261 agttggcacc atgttcaact cgatgacccc accaccaatc agtagctatg gcgagccctg
     1321 ctgtctccgg cccctcccca gtcagggggc ccccagtgtg gggacagaag gactgtctgg
     1381 cccgcccttc tgccaccaag ctaacctcat gtccggcccc cacagttatg ggccagccag
     1441 agagaccaac agctgcaccg agggcccact cttttcttct ccccggagtg cagtcaagtt
     1501 gaccaagaag cgggcactgt ccatctcacc tctgtcggat gccagcctgg acctgcagac
     1561 ggttatccgc acctcaccca gctccctcgt agctttcatc aactcgcgat gcacatctcc
     1621 aggaggctcc tacggtcatc tctccattgg caccatgagc ccatctctgg gattcccagc
     1681 ccagatgaat caccaaaaag ggccctcgcc ttcctttggg gtccagcctt gtggtcccca
     1741 tgactctgcc cggggtggga tgatcccaca tcctcagtcc cggggaccct tcccaacttg
     1801 ccagctgaag tctgagctgg acatgctggt tggcaagtgc cgggaggaac ccttggaagg
     1861 tgatatgtcc agccccaact ccacaggcat acaggatccc ctgttgggga tgctggatgg
     1921 gcgggaggac ctcgagagag aggagaagcg tgagcctgaa tctgtgtatg aaactgactg
     1981 ccgttgggat ggctgcagcc aggaatttga ctcccaagag cagctggtgc accacatcaa
     2041 cagcgagcac atccacgggg agcggaagga gttcgtgtgc cactgggggg gctgctccag
     2101 ggagctgagg cccttcaaag cccagtacat gctggtggtt cacatgcgca gacacactgg
     2161 cgagaagcca cacaagtgca cgtttgaagg gtgccggaag tcatactcac gcctcgaaaa
     2221 cctgaagacg cacctgcggt cacacacggg tgagaagcca tacatgtgtg agcacgaggg
     2281 ctgcagtaaa gccttcagca atgccagtga ccgagccaag caccagaatc ggacccattc
     2341 caatgagaag ccgtatgtat gtaagctccc tggctgcacc aaacgctata cagatcctag
     2401 ctcgctgcga aaacatgtca agacagtgca tggtcctgac gcccatgtga ccaaacggca
     2461 ccgtggggat ggccccctgc ctcgggcacc atccatttct acagtggagc ccaagaggga
     2521 gcgggaagga ggtcccatca gggaggaaag cagactgact gtgccagagg gtgccatgaa
     2581 gccacagcca agccctgggg cccagtcatc ctgcagcagt gaccactccc cggcagggag
     2641 tgcagccaat acagacagtg gtgtggaaat gactggcaat gcagggggca gcactgaaga
     2701 cctctccagc ttggacgagg gaccttgcat tgctggcact ggtctgtcca ctcttcgccg
     2761 ccttgagaac ctcaggctgg accagctaca tcaactccgg ccaataggga cccggggtct
     2821 caaactgccc agcttgtccc acaccggtac cactgtgtcc cgccgcgtgg gccccccagt
     2881 ctctcttgaa cgccgcagca gcagctccag cagcatcagc tctgcctata ctgtcagccg
     2941 ccgctcctcc ctggcctctc ctttcccccc tggctcccca ccagagaatg gagcatcctc
     3001 cctgcctggc cttatgcctg cccagcacta cctgcttcgg gcaagatatg cttcagccag
     3061 agggggtggt acttcgccca ctgcagcatc cagcctggat cggataggtg gtcttcccat
     3121 gcctccttgg agaagccgag ccgagtatcc aggatacaac cccaatgcag gggtcacccg
     3181 gagggccagt gacccagccc aggctgctga ccgtcctgct ccagctagag tccagaggtt
     3241 caagagcctg ggctgtgtcc ataccccacc cactgtggca gggggaggac agaactttga
     3301 tccttacctc ccaacctctg tctactcacc acagcccccc agcatcactg agaatgctgc
     3361 catggatgct agagggctac aggaagagcc agaagttggg acctccatgg tgggcagtgg
     3421 tctgaacccc tatatggact tcccacctac tgatactctg ggatatgggg gacctgaagg
     3481 ggcagcagct gagccttatg gagcgagggg tccaggctct ctgcctcttg ggcctggtcc
     3541 acccaccaac tatggcccca acccctgtcc ccagcaggcc tcatatcctg accccaccca
     3601 agaaacatgg ggtgagttcc cttcccactc tgggctgtac ccaggcccca aggctctagg
     3661 tggaacctac agccagtgtc ctcgacttga acattatgga caagtgcaag tcaagccaga
     3721 acaggggtgc ccagtggggt ctgactccac aggactggca ccctgcctca atgcccaccc
     3781 cagtgagggg cccccacatc cacagcctct cttttcccat tacccccagc cctctcctcc
     3841 ccaatatctc cagtcaggcc cctataccca gccaccccct gattatcttc cttcagaacc
     3901 caggccttgc ctggactttg attcccccac ccattccaca gggcagctca aggctcagct
     3961 tgtgtgtaat tatgttcaat ctcaacagga gctactgtgg gagggtgggg gcagggaaga
     4021 tgcccccgcc caggaacctt cctaccagag tcccaagttt ctggggggtt cccaggttag
     4081 cccaagccgt gctaaagctc cagtgaacac atatggacct ggctttggac ccaacttgcc
     4141 caatcacaag tcaggttcct atcccacccc ttcaccatgc catgaaaatt ttgtagtggg
     4201 ggcaaatagg gcttcacata gggcagcagc accacctcga cttctgcccc cattgcccac
     4261 ttgctatggg cctctcaaag tgggaggcac aaaccccagc tgtggtcatc ctgaggtggg
     4321 caggctagga gggggtcctg ccttgtaccc tcctcccgaa ggacaggtat gtaaccccct
     4381 ggactctctt gatcttgaca acactcagct ggactttgtg gctattctgg atgagcccca
     4441 ggggctgagt cctcctcctt cccatgatca gcggggcagc tctggacata ccccacctcc
     4501 ctctgggccc cccaacatgg ctgtgggcaa catgagtgtc ttactgagat ccctacctgg
     4561 ggaaacagaa ttcctcaact ctagtgccta attgccaact ttcttgtaca aagtggtggg
     4621 aggaggtgga tctggcgact acaaggacga cgatgacaag tgactcgagc gggatcaatt
     4681 ccgccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4741 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4801 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4861 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4921 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4981 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     5041 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     5101 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     5161 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5221 tttcctttga aaaacacgat aataccatgg tgagcaaggg cgaggagctg ttcaccgggg
     5281 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     5341 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     5401 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     5461 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     5521 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     5581 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     5641 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     5701 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     5761 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5821 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     5881 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5941 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagagtcgac ctgcaggcat
     6001 gcaagcttca ggtagccggc taacgttatc aagcttatcg ataatcaacc tctggattac
     6061 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6121 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6181 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6241 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6301 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6421 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6481 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6541 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6601 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     6661 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     6721 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     6781 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6841 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6901 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6961 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7021 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7081 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7141 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7201 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7261 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     7321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7441 ctagggacgt acccaattcg ccctatagtg agtcgtatta gcatgtgagc aaaaggccag
     7501 cataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     7561 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     7621 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     7681 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     7741 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     7801 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     7861 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7921 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7981 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8041 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8101 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8161 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8221 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8281 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8341 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8401 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8461 gtaactcgcc ttgatcgttg ggaaccgaag ctgaatgaag ccataccaaa cgacgagcgt
     8521 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8581 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8641 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8701 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8761 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8821 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8881 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8941 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9721 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     9781 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     9841 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     9901 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     9961 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    10021 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    10081 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
    10141 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
    10201 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
    10261 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
    10321 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10381 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10441 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
    10501 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
    10561 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
    10621 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    10681 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    10741 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    10801 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10861 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    10921 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    10981 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
    11041 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    11101 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    11161 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    11221 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    11281 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    11341 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    11401 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    11461 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    11521 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    11581 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    11641 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    11701 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    11761 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    11821 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
    11881 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
    11941 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    12001 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
    12061 tccagtttgg ttagtaccgg gcccgctcta gccctagatt taaatcgatg tacgggccag
    12121 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    12181 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    12241 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    12301 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    12361 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    12421 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    12481 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
    12541 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
    12601 ttgtgcagcg atgggggcgg gggggggg
//
