LOCUS       sequence-351471-       10353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for generation of ClonMapper gRNA barcode library for use 
            in droplet-based sequencing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Gardner A, Morgan D, Al'Khafaji A, Brock A
  TITLE     Functionalized Lineage Tracing for the Study and Manipulation of 
            Heterogeneous Cell Populations.
  JOURNAL   Methods Mol Biol. 2022;2394:109-131. doi: 
            10.1007/978-1-0716-1811-0_8.
  PUBMED    35094325
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2394:109-131. doi"; pages: " 
            10.1007/978-1-0716-1811-0_8"
COMMENT     Sequence Label: Crop-Seq-BFP-WPRE-TS-hU6-BsmbI
FEATURES             Location/Qualifiers
     source          1..10353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        149..397
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     149..169
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     320..339
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2279..2354
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             2407..2587
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2665..2786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2702..2721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2756..2775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2826..2961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2981..3000)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2982..3000)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3010..3027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3010..3026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3019..3041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3168..3623
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3255..3274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3465..3486
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3649..3753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3754..3822,3823..4614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3972..3991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4785..5373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5274..5293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5527..5544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5661..5682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5697..5702,5703..5720,5721..5727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5735..5751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5740..5762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5759..5775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5759..5775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5794..5814
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5796..5814
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5842..6068
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6069..6249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6296..6421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6914..7147
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7332..7376
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7557..7674
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7813..8991
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8044..8982
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8939..8959
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9721..10309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9774..9794)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10192..10203)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
       61 atttcaccat tatcgtttca gacccacctc ccaaccccga ggggacccaa gcagtggtat
      121 caacgcagag tacatggggg gacccagaga gggcctattt cccatgattc cttcatattt
      181 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa
      241 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      301 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      361 cttggcttta tatatcttgt ggaaaggacg aaacaccgga gacggttgta aatgagcaca
      421 caaaatacac atgctaaaat attatattct atgaccttta taaaatcaac caaaatcttc
      481 tttttaataa ctttagtatc aataattaga atttttatgt tcctttttgc aaacttttaa
      541 taaaaatgag caaaataaaa aaacgctagt tttagtaact cgcgttgttt tcttcacctt
      601 taataatagc tactccacca cttgttccta agcggtcagc tcctgcttca atcatttttt
      661 gagcatcttc aaatgttcta actccaccag ctgctttaac taaagcattg tctttaacaa
      721 ctgacttcat tagtttaaca tcttcaaatg ttgcacctga ttttgaaaat cctgttgatg
      781 ttttaacaaa ttctaatcca gcttcaacag ctatttcaca agctttcatg atttcttctt
      841 ttgttaataa acaattttcc ataatacatt taacaacatg tgatccagct gcttttttta
      901 cagctttcat gtcttctaaa actaattcat aatttttgtc ttttaatgca ccaatattta
      961 ataccatatc aatttctgtt gcaccatctt taattgcttc agaaacttcg aatgcttttg
     1021 tagctgttgt gcatgcacct agaggaaaac ctacaacatt tgttattcct acatttgtgc
     1081 cttttaataa ttctttacaa tagcttgttc aatatgaatt aacacaaact gttgcaaaat
     1141 caaattcaat tgcttcatca cataattgtt taatttcagc tttcgtagca tcttgtttta
     1201 ataatgtgtg atctatatat ttgtttagtt tcattttttc tcctatatat tcatttttaa
     1261 ttttaattct ttaataattt cgtctacttt aactttagcg ttttgaacag attcaccaac
     1321 acctataaaa taaattttta gtttaggttc agttccactt gggcgaacag caaatcatga
     1381 cttatcttct aaataaaatt ttagtaagtc ttgtcctggc atattataca ttccatcgat
     1441 gtagtcttca acattaacaa ctttaagtcc agcaatttga gttaagggtg ttgctctcaa
     1501 tgatttcatt aatggttcaa tttttaattt cttttcttct ggtttaaaat tcaagtttaa
     1561 agtgaaagtg taatatgcac ccatttcttt aaataaatct tctaaatagt ctactaatgt
     1621 tttattttgt tttttataaa atcaagcagc ctctgctatt aatatagaag cttgtattcc
     1681 atctttatct ctagctgagt catcaattac atatccataa ctttcttcat aagcaaaaac
     1741 aaaatttaat ccgttatctt cttctttagc aatttctcta cccattcatt taaatccagt
     1801 taaagttttt acaatattaa ctccatattt ttcatgagcg attctatcac ccaaatcact
     1861 tgttacaaaa cttgaatata gagccggatt ttttggaatg ctatttaagc gttttagatt
     1921 tgataatttt caatcaatta aaattggtcc tgtttgattt ccatctaatc ttacaaaatg
     1981 accatcatgt tttattgcca ttccaaatct gtcagcatct gggtcattca taataataat
     2041 atctgcatca tgtttaatac catattcaag cggtattttt catgcaggat caaattctgg
     2101 atttggattt acaacatttt taaatgtttc atcttcaaat gcatgctctt caacctcaat
     2161 aacgttatat cctgattcac gtaatatttt tggggtaaat ttagttcctg ttccattaac
     2221 tgcgctaaaa ataattttta aatctttttt agcttcttgc tcttttttgt acgtctctgt
     2281 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     2341 caccgagtcg gtgctttttt aagcttggcg taactagatc ttgagacact gctttttgct
     2401 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     2461 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     2521 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     2581 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     2641 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     2701 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2761 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     2821 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     2881 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2941 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     3001 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3061 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     3181 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3241 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3301 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3361 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3421 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3481 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3541 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3601 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     3661 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3721 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3781 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3841 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3901 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3961 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4021 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4081 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4141 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4201 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4261 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4321 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4381 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4441 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4501 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4561 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4621 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4681 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4741 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4801 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4861 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4921 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4981 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5041 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5101 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5161 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5221 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5281 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5341 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5401 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5461 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5521 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5581 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5641 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5701 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     5761 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     5821 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     5881 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     5941 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     6001 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     6061 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6241 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     6301 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     6361 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     6421 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     6481 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     6541 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     6601 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     6661 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     6721 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     6781 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     6841 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     6901 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     6961 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     7021 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     7081 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     7141 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     7201 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     7261 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     7321 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     7381 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     7441 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     7501 gatattcacc attatcgttt cagaatcttg agacaaatgg cagtattcat ccacaatttt
     7561 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     7621 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     7681 tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg ggtgcaaaga
     7741 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     7801 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     7861 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     7921 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     7981 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     8041 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     8101 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     8161 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     8221 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     8281 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     8341 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     8401 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     8461 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     8521 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     8581 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     8641 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     8701 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     8761 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     8821 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     8881 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     8941 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg acgtacggcc
     9001 accatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     9061 gacaaccacc acttcaagtg caccagcgag ggcgagggca agccctacga gggcacccag
     9121 accatgcgga tcaaggtggt ggagggcggc cccctgccct tcgccttcga catcctggcc
     9181 accagcttcc tgtacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     9241 ttcaagcaga gcttccccga gggcttcacc tgggagcggg tgaccaccta cgaggacggc
     9301 ggcgtgctga ccgccaccca ggacaccagc ctccaggacg gctgcctgat ctacaacgtg
     9361 aagatccggg gcgtgaactt caccagcaac ggccccgtga tgcagaagaa aaccctgggc
     9421 tgggaggcct tcaccgagac actgtacccc gccgacggcg gcctggaggg ccggaacgac
     9481 atggccctga agctggtggg cggcagccac ctgatcgcca acatcaagac cacctaccgg
     9541 agcaagaagc ccgccaagaa cctgaagatg cccggcgtgt actacgtgga ctaccggctg
     9601 gagcggatca aggaggccaa caacgagaca tacgtggagc agcacgaggt ggccgtggcc
     9661 cggtactgcg acctgcccag caagctgggc cacaagctga actgaacgcg ttaagtcgac
     9721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9841 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9901 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9961 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10021 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10081 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    10141 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10201 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10261 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
    10321 gaccaatgac ttacaaggca gctgtagatc tta
//
