LOCUS       sequence-351473-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pgref-1KPC-1 mini-gene unc-54 3' UTR C.elegans neuron and tissue 
            expression of KPC-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH3113
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    223..244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(259..264,265..282,283..289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    297..313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     302..324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     321..337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     321..337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7440..7457)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7440..7456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7449..7471)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7669..8124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7756..7775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7966..7987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8141..8160)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8260..8282
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8320..8338)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8406..8510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8511..8579,8580..9371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8729..8748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9542..10130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10031..10050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
       61 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      121 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      181 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      241 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      301 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt cgaatggcca
      361 tgggacgtcg acgcttgcat gcctgcagcg tttccgatac ccccttatat cagcacacat
      421 tcagtcatca taccacaaaa tgttgtgata gtgataggca aaactaccgt atttcctcta
      481 ttaatcttgc atgcaagact aattttcgat taacccgtag gggtgcaaga ctaatagaga
      541 ctgcaagact attagaggct gaaatactaa ttttcgtatg ctcaataatt ttggaaattg
      601 gcctattttt tgtagaaact tgataccgtt taaacaagga aaaatacaca ctttttaata
      661 tttcatcaat aatttgaacg attttgtgat tttaagttca atttgcccaa aaaaagacaa
      721 tttttctgac gttaccgcaa agtaatgctg gtcaggcaaa aagagcggtg caaaaatata
      781 agagactgca atactaatag aggaaatacg gtaattgaat tttagtgaaa cttgcgacag
      841 ttttcctcat ttttttgtta ttccggcata cgagtgtggc atacgagtga ggtcatcttt
      901 tgtttcttcc gtttcttcat cgcttttgaa aaaaaatgtt gaagaaaact tcgtagccga
      961 tagccggata atgtttggca cggcgttcca acatatatca ttggaaattt tttaagattt
     1021 ttgcgaaaaa tcacattctt cacgatgaga acacgttatt gaaggaatat aaatcaagaa
     1081 taacatatag ttatatttct ctattacttt aacgttaaat atgagcaaat ttgagcattt
     1141 tgattgcgat gaaaagcaga atcgagtcaa ctgaaatccg ttcaaaaacc taagctcggt
     1201 tcgcccgaaa gtcgattttt ccaatagccg aacagcactg cttctttatt ttccaggtat
     1261 ttttggggtg taagaattat atcttctttg tttgggtatt tacgtttccg aattcccttt
     1321 cttgatctgt cgctattttt cagaaaccag aacagtttat cctttttcat gagaacctga
     1381 atagctcaaa aacccgttta atttcttttc atcatctcat ttgaaacttt cctagtcttc
     1441 ttaatgattt ccaggctcct cctacatttg tatctcaaga tgcatacact ttatttccgc
     1501 tccaattcga atcatctact tctctttttt cttcaacttt ctactttttt ccattctttt
     1561 ctttcaaatg ttcgacgtct tcaagtgact gctcttcatt ctcctcattt ttctcgaatt
     1621 tcattcttgt ctcttttttg ctcaaaaatg gaaaatgaaa gtgacgtgag atttggacgg
     1681 cgggacacgg gggcagtaga agcagcaaaa aggagagaaa gaggacacaa ataagaagaa
     1741 cgaattcaaa aataagcgga gaggagctat ttccgtcaat tctacctccc caatcttcat
     1801 caattcggct caattgaatg acgtcacagg gagataaacc gtttggatga gcgccgtcca
     1861 tcactgacgc catcccgttt gggacaagaa aagagaaaaa agagcacaaa gtttttggtg
     1921 acggatcttg tcaatcatat gaaagttgtt ctgattgatt gtcagttttt ttcctacttt
     1981 tttggattct atccacttct gaacttttga caagtttcaa actttctgaa tcattttcta
     2041 tgcattttcc tggaattctt tttatgtaaa atatgaaata gaatgttttt gaattcaagt
     2101 tctgcttttt tcttcttttc tgttctgttc gggcttgggt atgctttttt taaaaattat
     2161 tttgcacatc gaccaataag tgcgcaactt ataaaattaa tttatttttg ttaatttttg
     2221 aaatacttgt attgctttaa gtgatctgac ctcgccctga gctttccacg tagttatcaa
     2281 atacaaatcc tccaagggta acgtacctat attactgatc tttataataa ctttatcacc
     2341 tgtccagttc cagaggaatt ctgttaagct tataggcaca gaaggagtca tttctgctgg
     2401 tttttaatga tccaaatctt tatttcaagt aaaaaactga acacttgcga ataaaactat
     2461 cagattaacc attcaccaaa aatgtgtttg aatctaaaac tttctcagta ttccaaatat
     2521 agaaataaat aaccacgaca ttgctaaaat ctgtctgaat tgtgtactcc ttaccgtgaa
     2581 gtaataatgg atataatgaa tcgtttgaaa tgaatgatca gcacattttt ggtgaaagat
     2641 cacaaataag gaataagcgt cggaaaataa aacgattatt tcggatcaaa aatttgttga
     2701 agatcatata cactcgagac caagattatt ctagacaatt ttcaaattgg cttctttgtt
     2761 tgcaaatctt cataataatc tgtgaagttt ggaaatttga atttttaatc ttttttcata
     2821 gattatagtt tttatttctt tgcaaaacta tattaaaaac cgatgcattg ttttagggaa
     2881 attaatgagc cttttgttca acactaaaaa caataaaatt aaaattttgg cttcatcatt
     2941 tgaccttttt taagttcgaa aacttttctc gtatttctga accgccaatt ttttcacaca
     3001 tctctagact tttggtgccc gttccagaaa gttaagtaat tgctattcta agaaagttct
     3061 caacattgtt ttttagttct gattgaattc tgatgttcca ggaatatatt ttaaatttaa
     3121 tattctttgc actatttcta tactaactaa ataataaata gtctaagtat gttcaatgag
     3181 aacaaaaaag ctctatctat atttttctat cctatttcat ttatatcctt ttcattttga
     3241 actcaccagc ttctcttctt cttcttgtcc atatacttct taaaagtctc gaacttctct
     3301 ctctcactct ccatcaattt ctttatggct accgcttgcg ctctgccgct tccgaagaaa
     3361 gaaggcgttg gcagagctct caattctatt ttttctcgcc gtaggctatc gatttctcag
     3421 cttctcttct ctcttcctca ctccaccacc accacttcgg gcttcttctt ttcttcattt
     3481 tcgtcttctt cttcatcctc ttcttttttt ttcagttccc cctcactcct ccccttatat
     3541 gcgcgtgcga gaggggtgca aaagcagcgc gcatccgaga attgaaacga gaaaacggag
     3601 acgcagcagc agttcgtcct cagaaaaata gccagtaaaa agagaaaaag atagagagag
     3661 acctatcgca ttttattttc aaattgcaat tcctgcaatt catgtgtgcg tgtgcccatt
     3721 ttcaattctt cccgttattt ttcagaccat taccaacgtt ctttctagat cttgagacaa
     3781 tcttccttct gctcaatcgt tcgtcgtgaa gacggcatcg acgacgacga cgatcagcag
     3841 taaaggatcc cccgggctgc aggaatgtca aatatcagtt ggtaccgtca ttgttcggtt
     3901 cggcttcaac tcgtcacact tgcacttttc cttttattag gctccgcctc tttaggctcc
     3961 gcccacattg atgaagagtt cgaagatgac gtcacgacga caatatcatc gattgcgtca
     4021 ccgatgaggc ggacgtacac aaatgagtgg gcggtgcgga tagcgggtgg gaaggttgaa
     4081 gaagcgaacc gactggcgaa caaatacggg tacacgaatt tgggtccgat aattccgggt
     4141 gacgagtact atttattccg tgacgatcga aaaaagagtc gatcgtcgcg aaaaacgcga
     4201 agcttatcag caaatcagct tcaacacgag gaagatgtca tgtggatgga acagcaagtg
     4261 gcgaagcgga gagtgaaaag aggatatcga aggattcgac gacatactga tgataatgat
     4321 atttttgaag aggatgatga tggtactcaa attagcaagt cgagaaatag aaaacatccg
     4381 gatccgaacg atccgttgtg gactgatatg tggtatttga atcgaggtga acatcattct
     4441 gactcaacaa cccgtatgga tcacaatgta aaagaagcct gggacctcgg gtacacagga
     4501 aaaggagtcg tcgtcacgat tcttgacgac ggtttggaaa gaactcaccc agatatttct
     4561 ccgaattacg atgaacgtgc ttcatatgac gtcaacgatc gagataatga cccgatgcca
     4621 agatacgaat tttcggatga aaatcgacat ggaactcggt gtgctggaga agttgcggcg
     4681 atttttaata atagtttatg tattgttgga atagcttaca atgcaaatat cggtggaatc
     4741 agaatgctcg atggtgacgt cacagatgcc gtcgaagccg cttccgttgg gcataatgca
     4801 gattacattg atatttatag cgcatcctgg ggaccggatg acgatggccg aaccgtcgac
     4861 ggccccgcaa aactcacaag aagtgccttc gaaaaaggaa tcaccatggg tcgaaaagga
     4921 aaaggctcaa tttttgtatg ggcatcagga aatggaggaa aagacgcgga ttcgtgtaat
     4981 tgtgatgggt acaccaactc catatacacg ttatccatat caagtgccac tgaaaatgga
     5041 aatatcccat ggtactctga agcttgcagt agtacccttg cgactaccta ctctagtgga
     5101 gcaactggcg aaaagatgat cctaaccaca gatctacacc acgcgtgcac aaatatgcac
     5161 acggggacca gtgcaagtgc tccgttggct gctggaattg ttgctcttgc tctagaagcc
     5221 aacccgaatc tcacgtggcg agaccttcaa catattgtga tacgaactgc aaagcctatc
     5281 aatttaagag caggagattg gactacaaat ggggttggga gaaatgtgtc ccatagtttt
     5341 ggttatgggt tgatggatgc tggagccatg gtgaagctgg caaagatctg gaagaaggtt
     5401 gatgagcagc atcggtgcag gcagttttat ccgtcgagat ataagtgagt agttatcacc
     5461 caaatttatg aaaaattcca gaagtttctc gaattttgca aaagttttca aaagtttcta
     5521 aacatttcta aaactttcct gctgttttca gaatttttaa gaattttccg ttttcaggag
     5581 ctattaaaat ttttttagac tttctagaag tttgtcaaac atttaaaaat gtttcctaat
     5641 actcgagaaa attttaaaaa tttactgaaa atccctgaga tcttcaaaaa tttttgacaa
     5701 gtttctagac gtttctcaaa tcttccagaa gtttatagaa gtctacagaa atttccaaaa
     5761 atttctcgat ttttccagaa gtttctagaa gtttcagaag ttcacacaat tttccagaaa
     5821 tttccaggga cttctacgcg ttttcagaag tttccgaaaa ttctagaaat tgagaaaatg
     5881 tttagaaatt ccaaaaaact ccaagttttt agaaatatcc ccaacggaaa ccgccttcaa
     5941 ctccaacttt actccgacgg atgttatggt ggagccgatg agaataaggt gtcttatgtg
     6001 gagcacgtgc aggcaatcgt cacattgaaa gctccaaaac gtggagattt gcaaatctac
     6061 ttgacttctc cgtcaggtaa tgattttgaa aataaattta ggatgtaaaa ctgacttttt
     6121 ttaaaccttc ataacttttt ttgacgaaaa gctagaacat tggctcaaaa agcgaaaatg
     6181 ctcagctgca aatttcaaat ttaaaaaaat ttaaaacgtt caggcacaaa atccacgttg
     6241 ctcacaaaaa gagcacgcga cacatcacga agtggcttca cagattgggc tttcatgacg
     6301 acccataact ggggggagca ggccgccggg ctgtggattc tggaaatcga taatgatgga
     6361 tgggatggtg agtattgatt tctgaaaatt cattgatttt taggagtgat tatcgatttt
     6421 tgcagacgcc gagcttgtaa aatgggaact cgtgctctac ggaactgatc gggaaaccgg
     6481 cgactttgga ggccaacatg cttcaccgtt agccgtcaga agtgtccaga tggaagccac
     6541 gtcatccggc acccaatatt caattttcca cgtcatcact cttgtcatat taacattttc
     6601 ccagatttta tattcctaag gtaccatggt attgatatct gagctccgca tcggccgctg
     6661 tcatcagatc gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca
     6721 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     6781 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     6841 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     6901 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     6961 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     7021 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     7081 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     7141 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     7201 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     7261 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     7321 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     7381 tataccaaac ataactgttt ctactagtgg ccacgtgggc cgtgcacctt aagcttggca
     7441 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7501 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7561 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     7621 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     7681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7861 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     7921 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7981 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     8041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     8101 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     8161 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     8221 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     8281 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     8341 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     8401 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8461 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8521 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8581 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8641 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8701 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8761 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8821 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8881 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8941 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9001 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9061 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctt
//
