LOCUS       sequence-351474-       11202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pdpy-30 DAF-2 unc-54 3' UTR C.elegans pan-tissue expression of DAF-2
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 11202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH4725
FEATURES             Location/Qualifiers
     source          1..11202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..49
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    166..187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(202..207,208..225,226..232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    240..256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     245..267
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     264..280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     264..280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8390..8407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8390..8406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8399..8421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8619..9074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8706..8725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8916..8937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9091..9110)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9210..9232
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9270..9288)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9356..9460
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9461..9529,9530..10321)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9679..9698)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10492..11080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10981..11000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
       61 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      121 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      181 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      241 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgaatgg
      301 ccatgggacg tcgacgcggc cgctctagac acgcgtgtaa cgtacaacat aaatgtttga
      361 cgtcaaccag atgtagtaca ataaaaaata tattcagatt acctagaaaa ttacttcggt
      421 catgaccacc aattttttga tttttcgatt ttcgaaaccg acatcgttga agaacattca
      481 ggtcataatt ttttatttga aaaataatgt tttctatgta ttaattcggt ttagtcgatc
      541 gaatatgtac agttgagaat tactagagta cggtgcgcaa gtatgcaaac ttgcgtaacg
      601 gtgtttacac atttgtcacg tttctctgcg tctcttcgtc tcaacaatgc ttgcactccg
      661 tcacactttc gaattgtcgg ccgatatcgg tatttagata aattcagtgc tctccttttt
      721 ttctccattt tactttcatc aatctttttt aaaaatcaat attcagcaaa ctattgaaaa
      781 attaattcaa aaaacacgac tggtcggaag ttatttgcat aatcaataga aatttcgttt
      841 tccgctcgct gtcagtgttt tgtcctgttt gtatttccca gtgcactttt cgagtggctc
      901 aagtttttat ctattttcct gcaaacttgc atatattgtt ttcttccaga agcctttgat
      961 atctcaataa ttttatactt gtttcaggga tcccccggga tgaatattgt cagatgtcgg
     1021 agacgacaca aaattttgga aaatttggaa gaagagaatc tcggcccgag ctgctcgtcg
     1081 acgacttcaa caaccgctgc caccgaagct ctcggaacaa ccactgagga tatgaggctt
     1141 aagcagcagc gaagctcgtc gcgtgccacg gagcacgata ttgtcgacgg caatcaccac
     1201 gacgacgagc acatcacaat gagacggctt cgacttgtca aaaattcgcg gacgcggcgt
     1261 agaacgacgc ccgattcaag tatggactgc tatgaggaaa acccgccatc acaaaaaact
     1321 tcaataaatt attcttggat ttctaaaaag tcatcaatga cgtcattaat gcttttactg
     1381 ctattcgctt ttgtacagcc gtgtgcctca atagtcgaaa aacgatgcgg cccaatcgat
     1441 attcgaaata ggccgtggga tattaagccg caatggtcga aacttggtga tccgaacgaa
     1501 aaagatttgg ctggtcagag aatggtcaac tgcacagtgg tggaaggttc gctgacaatc
     1561 tcatttgtac tgaaacacaa gacaaaagca caagaagaaa tgcatcgaag tctacagcca
     1621 agatattccc aagacgaatt tatcactttt ccgcatctac gtgaaattac tggaactctg
     1681 ctcgtttttg agactgaagg attagtggat ttgcgtaaaa ttttcccaaa tcttcgtgta
     1741 attggaggcc gttcgctgat tcaacactat gcgctgataa tttatcgaaa tccggatttg
     1801 gagatcggtc ttgacaagct ttccgtaatt cgaaatggtg gtgtacggat aatcgataat
     1861 cgaaaactgt gctacacgaa aacgattgat tggaaacatt tgatcacttc ttccatcaac
     1921 gatgttgtcg ttgataatgc tgccgagtac gctgtcactg agactggatt gatgtgccca
     1981 cgtggagctt gcgaagagga taaaggcgaa tcaaagtgtc attatttgga ggaaaagaat
     2041 caggaacaag gtgtcgaaag agttcagagt tgttggtcga acaccacttg ccaaaagtct
     2101 tgtgcttatg atcgtcttct tccaacgaaa gaaatcggac cgggatgtga tgcgaacggc
     2161 gatcgatgtc acgatcaatg cgtgggcggt tgtgagcgtg tgaatgatgc gacagcatgc
     2221 cacgcgtgca agaatgtcta tcacaaggga aagtgtatcg aaaagtgtga tgctcacctg
     2281 taccttctcc ttcaacgtcg ttgtgtgacc cgtgagcagt gtctgcagct gaatccggtg
     2341 ctctcgaaca aaacagtgcc tatcaaggcg acggcaggcc tttgctcgga taaatgtccc
     2401 gatggttatc aaatcaaccc ggatgatcat cgagaatgcc gaaaatgcgt tggcaagtgt
     2461 gagattgtgt gcgagatcaa tcacgtcatt gatacgtttc cgaaggcaca ggcgatcagg
     2521 ctatgcaata ttattgacgg aaatctgacg atcgagattc gcggaaaaca ggattcggga
     2581 atggcgtccg agttgaagga tatatttgcg aacattcaca cgatcaccgg ctacctgttg
     2641 gtacgtcaat cgtcaccgtt tatctcgttg aacatgttcc ggaatttacg acgtattgag
     2701 gcaaagtcac tgttcagaaa tctatatgct atcacagttt ttgaaaatcc gaatttaaaa
     2761 aagctattcg attcaacgac ggatttgacg cttgatcgtg gaactgtgtc aattgccaat
     2821 aacaagatgt tatgcttcaa gtatatcaag cagctaatgt caaagttaaa tataccactc
     2881 gatccgatag atcaatcaga agggacaaat ggtgagaagg caatctgtga ggatatggca
     2941 atcaacgtga gcatcacagc ggtcaacgcg gactcggtct tctttagttg gccctcattc
     3001 aacattaccg atatagatca gcgaaagttt ctcggctacg agctcttctt caaagaagtc
     3061 ccacgaatcg atgagaacat gacgatcgaa gaggatcgaa gtgcgtgtgt cgattcgtgg
     3121 cagagtgtct tcaaacagta ctacgagacg tcgaacggtg aaccgacccc ggacattttt
     3181 atggatattg gaccgcgcga gcgaattcgg ccgaatacgc tctacgcgta ctatgtggcg
     3241 acgcagatgg tgttgcatgc cggtgcgaag aacggtgtat cgaagattgg ttttgtgagg
     3301 acgagctact atacgcctga tcctccgacg ttggcactag cgcaagtcga ttcggacgct
     3361 attcatatta cgtgggaagc gccgctccaa ccgaacggag acctcacgca ttacacaatt
     3421 atgtggcgtg agaatgaagt gagcccgtac gaggaagccg aaaagttttg tacagatgca
     3481 agcacccccg caaatcgaca acacacgaaa gatccgaaag agacgattgt agccgataag
     3541 ccagtcgata ttccgtcatc acgtaccgta gctccgacac ttttgactat gatgggtcac
     3601 gaagatcagc agaaaacgtg cgctgcaacg cccggttgtt gttcgtgttc ggctatcgaa
     3661 gaatcatcgg aacagaacaa gaagaagcga ccggatccga tgtcggcgat cgaatcatct
     3721 gcatttgaga ataagctgtt ggatgaggtt ttaatgccga gagacacgat gcgagtgaga
     3781 cgatcaattg aagacgcgaa tcgagtcagt gaagagttgg aaaaagctga aaatttggga
     3841 aaagctccaa aaactctcgg tggaaagaag ccgctgatcc atatttcgaa gaagaagccg
     3901 tcgagcagca gcaccacatc cacaccggct ccaacgatcg catcaatgta tgccttaaca
     3961 aggaaaccga ctacggtgcc gggaacaagg attcggctct acgagatcta cgaaccttta
     4021 cccggaagct gggcgattaa tgtatcagct ctggcattgg ataatagtta tgtgatacga
     4081 aatttgaagc attacacact ttatgcgatt tctctatccg cgtgccaaaa catgacagta
     4141 cccggagcat cttgctcaat atcccatcgt gcgggagcat tgaaacgaac aaaacacatc
     4201 acagacattg ataaagtgtt gaatgaaaca attgaatgga gatttatgaa taatagtcaa
     4261 caagtcaacg tgacgtggga tccaccgact gaagtgaatg gtggaatatt cggttatgtt
     4321 gtaaagctta agtcaaaagt cgatggatca attgttatga cgagatgtgt cggtgcgaag
     4381 agaggatatt caacacggaa tcagggtgtc ctattccaga atttggccga tggacgttat
     4441 tttgtctcag taacggcgac ctctgtacac ggcgctggac cggaagccga atcctccgac
     4501 ccaatcgtcg tcatgacgcc aggcttcttc actgtggaaa tcattctcgg catgcttctc
     4561 gtctttttga ttttaatgtc aattgccggt tgtataatct actactacat tcaagtacgc
     4621 tacggcaaaa aagtgaaagc tctatctgac tttatgcaat tgaatcccga atattgtgtg
     4681 gacaataagt acaatgcaga cgattgggag ctacggcagg atgatgttgt gctcggacaa
     4741 cagtgtggag agggatcatt cggaaaagtg tacctcggaa ctggaaataa tgttgtttct
     4801 ctgatgggtg atcgtttcgg accgtgtgct attaagatta atgtagatga tccagcgtcg
     4861 actgagaatc tcaactatct catggaagct aatattatga agaactttaa gactaacttt
     4921 atcgtcaaac tgtacggagt tatctctact gtacaaccag cgatggttgt gatggaaatg
     4981 atggatcttg gaaatctccg tgactatctc cgatcgaaac gcgaagacga agtgttcaat
     5041 gagacggact gcaacttttt cgacataatc ccgagggata aattccatga gtgggccgca
     5101 cagatttgtg atggtatggc gtacctggag tcgctcaagt tttgccatcg agatctcgcc
     5161 gcacgtaatt gcatgataaa tcgggatgag actgtcaaga ttggagattt cggaatggct
     5221 cgtgatctat tctatcatga ctattataag ccatcgggca agcgtatgat gcctgttcga
     5281 tggatgtcac ccgagtcgtt gaaagacgga aagtttgact cgaaatctga tgtttggagc
     5341 ttcggagttg ttctctatga aatggttaca ctcggtgctc agccatatat tggtttgagt
     5401 aatgatgagg tgttgaatta tattggaatg gcccggaagg ttatcaagaa gcccgaatgt
     5461 tgtgaaaact attggtataa ggtgatgaaa atgtgctgga gatactcacc tcgggatcgt
     5521 ccgacgttcc tccagctcgt tcatcttcta gcagctgaag cttcaccaga attccgagat
     5581 ttatcatttg tcctaaccga taatcaaatg atccttgacg attcagaagc actggatctt
     5641 gatgatattg atgatactga tatgaatgat caggttgtcg aggtggcacc ggatgttgag
     5701 aacgtcgagg ttcagagtga ttcggaacgt cggaatacgg attcaatacc gttgaaacag
     5761 tttaagacga tccctccgat caatgcgacg acgagtcatt cgacaatatc gattgatgag
     5821 acaccgatga aagcgaagca gcgagaagga tcgctggatg aggagtacgc attgatgaat
     5881 catagtggag gtccgagtga tgcggaagtt cggacgtatg ctggtgatgg agattatgtg
     5941 gagagagatg ttcgagagaa tgatgtgcca acgcgacgaa atactggtgc atcaacatca
     6001 agttacacag gtggtggtcc atattgccta acaaatcgtg gtggttcaaa tgaacgagga
     6061 gccggtttcg gtgaagcagt acgattaact gatggtgttg gaagtggaca tttaaatgat
     6121 gatgattatg ttgaaaaaga gatatcatcc atggatacgc gccggagcac gggcgcctcg
     6181 agctcttcct acggtgttcc acagacgaat tggagtggaa atcgtggtgc cacgtattat
     6241 acgagtaaag ctcaacaggc agcaactgca gcagcagcag cagcagcagc tctccaacag
     6301 caacaaaatg gtggtcgagg cgatcgatta actcaactac ccggaactgg acatttacaa
     6361 tcgacacgtg gtggacaaga tggagattat attgaaactg aaccgaaaaa ttatagaaat
     6421 aatggatctc catcggtact ggattttttt ttttggagaa attagccgga aaattcgatt
     6481 tttttcgctg aaaatctgaa aaaatttggc taaaaattgc cctgaaacac gtttttttcg
     6541 gaattttcat ataaaaatgg acattttcgt aaaaaattgg ctatgtgttg tgaatttttt
     6601 ctgactttta aaggcctttg aactaggaat attaaaattt tattgaaaat tattgaaaag
     6661 tgcaaatttt tatttttttt aggtgaaaaa agcttagatt taggcgaatt ttctgaaatt
     6721 ttatagtgaa tttggttctc aggatattag ccattttttc ccgatttttt ggtaaaaatt
     6781 tgttaaaacc cgcttcaaat gactgtagtt ttatacaaaa ataactaaaa attcgatttt
     6841 tcaattattc agctcgaaaa agcccaacaa aaatatttaa aatttgaaga aaaaagctaa
     6901 aaatgagtaa aaatgactgg tttttttcgc tttttctatt tgaataaatt tctgaaaaat
     6961 gacgaaaatc tgaaaaaatt accccggaca ttgggaaatt tcgaaaaaat cttgaaaata
     7021 gtctgaaatt tttaaaattt acagattttc acaaaaaagg gaaatttcgc atttcctgaa
     7081 attgcccaaa aatttttttt ttcatttttt taaaaattct ggaaatttat ttttggctgt
     7141 ttcaaatttt taaaaaaaga aaaatcccaa aaattggtga aaactccatt aaaattctcc
     7201 agaaaattca aattttccat gaaaaattgt tttaaaagca tgaaaatctg ccgatttttg
     7261 tctaaaattt ttaaaaatat ttcaaaaatt tcagaaaaaa caggaaaagc gaatattaca
     7321 taaataaaaa atctggaaat tctagaaact gaaaattttt taatttttcc gctcaaaaat
     7381 tctaatattc gatgaaaatg agcccgaaaa ttcgagtaaa tttccaaaaa aaattttttt
     7441 ttttttcaaa cacaaatatt aatttttatt aatttttcag cgaaacggca acagccgtga
     7501 cattttcaac ggacgttcgg ctttcggtga aaatgagcat ctaatcgagg ataatgagca
     7561 tccacttgtg gtaccatggt attgatatct gagctccgca tcggccgctg tcatcagatc
     7621 gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     7681 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
     7741 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     7801 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     7861 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     7921 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     7981 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     8041 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
     8101 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
     8161 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
     8221 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
     8281 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
     8341 ataactgttt ctactagtgg ccacgtgggc cgtgcacctt aagcttggca ctggccgtcg
     8401 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8461 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8521 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     8581 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     8641 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8701 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8761 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8821 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8941 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     9001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     9061 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9121 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     9181 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     9241 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     9301 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     9361 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9421 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9481 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9541 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9601 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9661 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9721 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9781 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9841 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9901 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9961 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    10021 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    10081 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    10141 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10201 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10261 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10321 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10381 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10441 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10501 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10561 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10621 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10681 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10741 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10801 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10861 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10921 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10981 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    11041 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    11101 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11161 tgattctgtg gataaccgta ttaccgcctt tgagtgagct ga
//
