LOCUS       sequence-351475-        8388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Psra-11::D3cpv unc-54 3' UTR C.elegans neural expression of Psra-11 
            D3cpv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH1973
FEATURES             Location/Qualifiers
     source          1..8388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2951..2953,2954..2956,2957..3469)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4175..4909
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     complement(5828..5850)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5950..5969
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6047..6065)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6133..6237
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6238..6306,6307..7098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6456..6475)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7269..7857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7758..7777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8011..8028
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8145..8166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8181..8186,8187..8204,8205..8211)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8219..8235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8224..8246
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8243..8259
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8243..8259
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgcacgt tttcccttta cattagcaaa accgaccaac
       61 gaccgaaacc taagcattat ccaggcctaa acatatttta aaaaaagtgt agtttaccaa
      121 atgggagaca ttttccttgt tctttgtggg atgcttttga aattgaatag aaatctctag
      181 attaatcaat gtgtttgctc cgatgcgcct agtctcaaag ccttctcatg ttgcaaacaa
      241 atgcactaac taaactaatt aacaagtcac ggaggagatt caaaacattt cacttttcag
      301 gaaagtagca tttttaaata tattcatgtt gtagatttgt cattttgcaa tattttttat
      361 caaaattgga ttacaccctc ttttgagcag tctgacttgt tggctgactt gattttcttt
      421 taaagtgatg aaattttatt catatttgat ttttagtaaa taaattattg caaaccccat
      481 tatgcaactt tgacagctta tatctcagtt gccgtttgtt ttatgaaaaa ttgtcaactt
      541 accaaataat tcttagttac gatctgaaat ttttcgaaaa ctctaatctt tagtcaaaaa
      601 tgttcagcaa tgtaaacatt ttttcattac aaaaaaagat gatgtagatg catacatcaa
      661 gtatctcaac cggaacaccc cctattttgc accatctctg tcatcttttt cttccttttt
      721 cagatgggaa acatacaatt aatgctcaca tgtcatgtct agtcatttgt ttttttttgt
      781 ttgtttttaa tccgaatctc ttattcatct ttagacgttc cattcggcag aggcgagttg
      841 gggcgcctct tactcgcctc aaattttgtg tttgcactca taatcaaaga ataaattatc
      901 ttgcccattt tggtccttga ccatttgtgt aggcaatagg caggcagaca tattttctta
      961 aacagaagac cgaactgtga gtgtacaaaa ataaattaaa tattacctac ctttgttaga
     1021 atttggaata agattaaaaa attcgttgtc ctctgtggaa ttgctcatta ttagattttt
     1081 acgaaatcat gcggattaga aagtttaata tttatgtaca gtgcgtccaa cttctatagc
     1141 acccccctcc aattcggccg tttggagctt ttcaaaatat ttttcggaaa agtgttagtg
     1201 cagttcgata gcaaatattg aaaaacctat gctccccaat ttttatcatg atatctcaaa
     1261 aatcacggaa attgggtcaa aatatctgaa aaatggccgt cgaacttcta tagcaccccc
     1321 tctgattttt gacaaatttc tgtaaattcg gggttttctt cgtagttaat gaatttattt
     1381 tgtaaataac ttcacaccaa aataatcagg tggtttcact ttcacattta aaaaataaat
     1441 ataacacttg aaaacacgtg gaatgcctgg gattgatcaa aaattgatca aaatgttctt
     1501 ctccgtctat tcattactat gtaattagtt gtagaaagca tttattcata tgaaccaaat
     1561 ggtaaaggga gcaacttcag tggtttttaa aaggctcgat ttaaccatga aaacgagcgt
     1621 tcggaggcac aaagctgaag gttttgactt cgtattgtac agaacttcag ctcatcctgc
     1681 tcaaatgtct ttgaagcttc gattttgatt agatgtcttt gaaaatttat acggaaaaga
     1741 tcatgagacc gctcaaaaag aggacaaagg caaagcgagg ccgcaagtct ttttttcaag
     1801 ctaacatgcg aaaattttag acagaaaagt aagatatacc tttccaaatg acgattttgc
     1861 tagttgtatt tataactcaa gttaaattta gcgaattcgc tcattgaatc tttttttggt
     1921 caagtaatgc aaaagctacc agacaaaaca ttgaacctgc gattgtagat gtctggcatc
     1981 tgatttaccc atggatttcc acggattttt tgttcatata aaataatttt taatttttgc
     2041 tataatgtag taatttttag tcgaaaatgt attcccaacc attatctctg cgcctccgaa
     2101 cgctcgtttt catggttaaa tcgaaccttt taaaaaccac tgaagttgct ccctttacca
     2161 tttggttcat atgaataaat gctttctaca actaattata tagtaatgaa tagacggaga
     2221 agaacatttt gatctatttt ggtgctgcga aaatcattgg gagccaagat atggcctctg
     2281 aagcaaataa gaacgttgag gggggtgcta tagaagttgg acgcactgta tttccaatta
     2341 ttttttaaga atttcgattt tataaacagc ctttgctccg cctatttgag ctcatgatgt
     2401 ttctcttttg ttttcaagat tgttgattcc ttcctattgt cctattgtga tttttaaatt
     2461 tttaaattgc tcattaactt gatttttatt tagtacatat ctctaatatg ttgtttcaca
     2521 ttgcaccaac cggtctcaat ttttgaaatt atttattgtt atgtttgaat attttaaaca
     2581 aatttaaaaa tttagaaaaa ttgctgagct gttgcctaaa ctcataagtg tttcaaaatt
     2641 tgctacgatt ttgcatgtac tttttaatat tttatatgct ttttcgtatt ccaaaacaaa
     2701 caatttagtt tactttacaa attatgaaca aaaaaaattt gatgtaacgg agagaaaatt
     2761 attaaacgtt acatgtattt ttcgatcgta tcgagaaaaa tgcaaaaaat atcaaattgt
     2821 aatttttccg cacatctccg caacacacaa ttcccgggat tggccaaagg acccaaaggt
     2881 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc
     2941 gacggatccc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3001 cgagctggac ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga
     3061 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3121 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga
     3181 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3241 taccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3301 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3361 cctggggcac aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa
     3421 gcagaagaac ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcagcgt
     3481 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3541 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3601 tcacatggtc ctgctggagt tcgtgaccgc cgcccgcatg catgaccaac tgacagaaga
     3661 gcagattgca gagttcaaag aagccttctc attattggac aaggatgggg acggcaccat
     3721 caccacaaag gaacttggca ccgctctgag gtcgcttgga caaaacccaa cggaagcaga
     3781 attgcaggat atgatcaatg aagtcgatgc tgatggcaat ggaacgattt actttcctga
     3841 atttcttact atgatggcta gaaaaatgaa ggacacagac agcgaagagg aaatccgaga
     3901 agcattccgt gtttttgaca aggatgggaa cggctacatc agcgctgctg aattacgtca
     3961 cgtcatgaca aacctcgggg agaagttaac agatgaagaa gttgatgaaa tgataaggga
     4021 agcagatatc gatggtgatg gccaagtaaa ctatgaagag tttgtacaaa tgatgacagc
     4081 aaaggggggg aagaggcgct ggcagaaaac aggccatgct gtccgagcat tcggaagact
     4141 gaagaagatc tccagctccg gggcactgga gctcatggac ggcggcgtgc agctcgccga
     4201 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4261 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4321 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg
     4381 cagcggtggc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     4441 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     4501 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
     4561 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
     4621 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4681 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4741 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4801 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
     4861 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagt aagaattcca
     4921 actgagcgcc ggtcgctacc attaccaact tgtctggtgt caaaaataat aggggccgct
     4981 gtcatcagag taagtttaaa ctgagttcta ctaactaacg agtaatattt aaattttcag
     5041 catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct
     5101 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc
     5161 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     5221 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     5281 tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa
     5341 atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt
     5401 cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca
     5461 tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat
     5521 gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga
     5581 ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg
     5641 aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg
     5701 agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat
     5761 aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg
     5821 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     5881 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     5941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     6001 cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc
     6061 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6121 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6181 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6241 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6301 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6361 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6421 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6481 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6541 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6601 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6661 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6721 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6781 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6841 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6901 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6961 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7021 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7081 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7141 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7201 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7261 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7321 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7381 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7441 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7501 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7561 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7621 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7681 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7741 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7801 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7861 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7921 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7981 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8041 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8101 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8161 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8221 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa
     8281 gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
     8341 ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaat
//
