LOCUS       sequence-351476-        8683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnmr-1::D3cpv unc-54 3' UTR C.elegans neural expression of 
            Pnmr-1D3cpv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH1969
FEATURES             Location/Qualifiers
     source          1..8683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3246..3248,3249..3251,3252..3764)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4470..5204
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     complement(6123..6145)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6245..6264
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6342..6360)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6428..6532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6533..6601,6602..7393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6751..6770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7564..8152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8053..8072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8306..8323
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8440..8461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8476..8481,8482..8499,8500..8506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8514..8530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8519..8541
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8538..8554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8538..8554
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgctgtg gtctcacttt gaacgacaaa agaaaaccaa
       61 atattgtaaa ggaatagtac agataaaagg gaacaagtga taccagctga tggatgtagg
      121 tcagcgacaa aagacaggag acgtatagtt tgatgattat ggaaccaaac tcagaattta
      181 atgttggaga cgctataaaa attgaattga gaaattgaaa ccatattctt gaatatctct
      241 caatttggaa tttggaagta gccgtaaaat cggctagcta gaacataaaa ggactgattt
      301 gcaaccttga ccattcatga tatcatttca ataaaagaag agattccaat gaccaatctt
      361 caagaatcac aataatcaca acaaaactaa gcaaaagtaa caacaaattg ttcaggtaga
      421 caagtagaca tttggacaca ttttgcgggg atgtgatgac tgtataataa aaataagtgg
      481 acactgagag agagaggagg tcagcaggaa aaaagaacat cgaactgctt gacgaattga
      541 aattagaatg aggtgtcagg caggtgacga ttacatggtt aattattagt gttctttttc
      601 ttctttttgt ggatgccgat tagttgaaat aattgcatgg aaagtgagaa ttcaatgtga
      661 atctctttct tattatgttt caagtggtat ctggatggaa gcttgtactt ttatgaatcg
      721 aaaattgcgc ccaaggttga ttttgagatt attcagtaaa tttttgtgct gattattcag
      781 ttaatgtaga acagagacgc ggatgaaaac ttttttaatt tctaaaaaaa aagacaaaat
      841 gtggttaaaa agtttggaaa ataattgtta tatacttctt gagagcttat tcaaacggaa
      901 accaatcata aatagtcgaa tagtcattta gaaaagagaa actagttgat gtagagagat
      961 tcaaaaagta aaatatgcaa atgatccgtt tcttagattt agtaaacatc taccaaatga
     1021 aacaggaatt gtcgaaacaa tttagaaaag gagaaggaaa catttgttta ttttcccctt
     1081 taaatggcgg tagaggtaaa acagcaatac acatagtaca aggttatact actttcaaaa
     1141 gaatttgaca tcccgccgag aaaatgtggt gattatatgg taaagttttc agttggatat
     1201 tatcttctac agacggtgat tcaaaatgat caaaagggca agtgggtggt aggatttgaa
     1261 aaattgagat atagaacgtg cgattttggt aaaacttttt gaacacaagg catatttttg
     1321 aactttgaaa aacgcaaaat ttaaattttg ttaattacat atattttgaa cagattatga
     1381 atataagcaa ggctacataa aatatgtata ttttcttttt acaaatattc gtacactgtt
     1441 aaatttatag aaaaatcatt tatattcaga aaaacctatt tgaacaattt ttagttgctc
     1501 aataaccaat ttgcacaaat ttagaaaata taatgggggc ttttaaaaat ttaaatcctc
     1561 agcgaatatt cctttgcgat tttttcgaac gttttctttc agattaaaaa atctgaagca
     1621 gaagtaaatg aactaccata gaaaattcac caaggatcac atttttacaa agcctccgcc
     1681 caattgtaaa cttgggaatt tttgcttgtt ttcttttttt tttcaaaaaa ggaatagttg
     1741 ttttaaagta atttattccc ctatgctttc tccagcaatc agttgttctc cccatacttt
     1801 acacaattat caacaacttc acatagaaaa caaaacaaca aatcagaaat tcccaccggt
     1861 cacagtcaca gattccccat gttttgttca tctcatttct gcaagaaagt gacatcgctg
     1921 ccacccaatt cgacaaattg tccaagtggt catcaggaag atatgacctt ctttttaatg
     1981 ataatttaga ctacaaaggg cgtcaaaaaa gattcaatga tcaggggtaa ttgctgtaaa
     2041 atgtagtttg tatgtgtgtt taccgtagcc tataactgta aaaattggtt ttttaaaaag
     2101 tatttctgag attaaaaggc tccccaagga tgcacgattc tagctagaaa tgaacattcc
     2161 atgggttgtg aagatgcttt tatctcactt tagaatgtct gactccgccc ctctgtgtac
     2221 gaattgaagt gctcttttgt gatgatcata gctcctcctt gcggcgacgc ccgcacacag
     2281 cacgagatat tacacggaat acttatgtga ttcataggaa acaaggaaat cgaaaaaata
     2341 ataggatttt gaggaggtat ttttagatga atgcttatga tgttatgatg aacccaagaa
     2401 attctgaata gacatatgtc agaacggggt aggagctcca gtgtcgcttt tgaaataaaa
     2461 aatcaagtgg tatacttctc gtatataccc ttctgaacta ataaaataat ttatcttttg
     2521 aaaaagtttg aaaaagaaaa atgcccaaaa cttgaatttt aatgttttgt attttgagca
     2581 tatcgttcaa acgtcgtgat ctgaaagtag aaagatctaa atattcgtga cacagtttgg
     2641 tttgaaggtc ttagtggtaa ttttttatta cagaagcaac aaaaagttga catattttca
     2701 gaatttccaa aaaaacgttt aaaatttcaa tttcttcgat agagttctaa aaattttttg
     2761 gaacggattt tgtaatatcc gctgataacc gttcaagaac aatttgaatt ttgaaataat
     2821 ttaatcaact agtatccagt gctctttctc actgctccac ccatttttca acccatttcc
     2881 tttactcaat ttcgcccgtt ttccttttgc tcaaatgtgc ccaccaaatc atctttcttc
     2941 gtcttctttt ctcttattcg gaaggagaag aggtgaaagt agaataagtt tttttgttga
     3001 aaagagagga gcacggccac ttttctctta tttccatcct tctactacct cgttttactt
     3061 tccatttaaa aacccgttta aagttccaaa atttcaatta tcttgcttct gtttggaaca
     3121 cgacaaactt tagttttgtt acagataccc gggattggcc aaaggaccca aaggtatgtt
     3181 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta gcgtcgacgg
     3241 atcccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3301 tggacggcga cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca
     3361 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3421 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca
     3481 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgtacca
     3541 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3601 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3661 ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga
     3721 agaacggcat caaggcccac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3781 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3841 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3901 tggtcctgct ggagttcgtg accgccgccc gcatgcatga ccaactgaca gaagagcaga
     3961 ttgcagagtt caaagaagcc ttctcattat tggacaagga tggggacggc accatcacca
     4021 caaaggaact tggcaccgct ctgaggtcgc ttggacaaaa cccaacggaa gcagaattgc
     4081 aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt cctgaatttc
     4141 ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc cgagaagcat
     4201 tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctgaatta cgtcacgtca
     4261 tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata agggaagcag
     4321 atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg acagcaaagg
     4381 gggggaagag gcgctggcag aaaacaggcc atgctgtccg agcattcgga agactgaaga
     4441 agatctccag ctccggggca ctggagctca tggacggcgg cgtgcagctc gccgaccact
     4501 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4561 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4621 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggcagcg
     4681 gtggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4741 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4801 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     4861 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     4921 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4981 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     5041 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     5101 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     5161 agaacggcat caaggccaac ttcaagatcc gccacaacat cgagtaagaa ttccaactga
     5221 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat
     5281 cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct
     5341 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     5401 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     5461 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     5521 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     5581 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     5641 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     5701 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     5761 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     5821 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     5881 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     5941 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     6001 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     6061 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6121 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6181 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     6241 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     6301 agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt
     6361 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6421 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6481 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6541 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6601 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6661 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6721 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6781 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6841 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6901 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6961 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7021 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7081 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7141 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7201 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7261 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7321 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7381 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7441 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7501 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7681 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8221 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8281 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8341 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8401 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8461 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8521 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta
     8581 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
     8641 atcactcaca acgatggata cgctaacaac ttggaaatga aat
//
