LOCUS       sequence-351477-       12025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 
            YC3.60.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 12025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH1579
FEATURES             Location/Qualifiers
     source          1..12025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(5574..5576,5577..5579,5580..6092)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             6714..6791
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             6798..7532
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             8099..8350
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(9153..9170)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9153..9169)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9162..9184)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9382..9837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9469..9488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9679..9700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9854..9873)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9973..9995
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10033..10051)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10119..10223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10224..10292,10293..11084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10442..10461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11255..11843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11744..11763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11997..12014
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgaatgg
      241 ccatgggacg tcgacgcggc cgctgcagca ttttttaaaa gtttttctgt agccggtatg
      301 cactgataac tacatatctc tgggttttat actacattcc gcaaatatgt atttgttttt
      361 gctcaaaatg cacaatatag cgtggctagt ttcaacaccg tttcaaagtt tcaaaatttt
      421 caaaatcgat aaaatgagtt gcgacgtgaa attttttccc ttaaccatta aatagatttc
      481 attttcttct ctttttgaga aagttccaca ttttttgtta tgtgatttgt tagctaaacg
      541 taaatcgatt aggaatgtat cgtcgcattt gccagtgttg caggttgggt ttttttaaac
      601 atattttacg tttcagactt gcacatattt tcttgaatag aatgaaaaat gttcaacagt
      661 aaaaattaat gaagaaaact atttaacgta tattggactt cagaaccagc cgcgtgtcat
      721 tcgaattctt taaaaaaatt agattttgac tatagtccta taggtcacca caaaaattgt
      781 aacggcggaa ctcaaactaa acaacagaaa attcaatttt aaattttcag taaaatttct
      841 ctggtgatcg tagaattgcc tagaagatgt tattgagaca aacgttctga atgttgtttg
      901 gatttttttt tgttgatcga tcgagataaa tgtaacaatt tacaagatag aaagaatatg
      961 gtagattaat cacgaaaaaa acctcaaaat agcttacttc agtttttaga aagttaatct
     1021 tcagtatact ctctttgata tattttttct ccagatattc tgctgtctca ctgtaatacg
     1081 caattcaaat attttcaggt ttccaagcat gtcaaattat aataagccaa ttcaattact
     1141 ttgtgtgttg gttgtaggtg cgtttatttt caaaacttta ttttatgatt atattttatt
     1201 cagttttatt ctttatcaac aatcaatttg ttcaaaggac tacgtggagt cgagggctca
     1261 gatcaccgtt agttgaaagg tttcaaattt tgtaccgttt gtagcgtcag tggagatatt
     1321 tctgctccac ttgcgctgcc cagcaaattt aaatttgaaa tatgtacatt ttatttgaag
     1381 aaaacgtctg taaatctcga ttttcagacc cctagattcg aggatttttc aagaaaagtc
     1441 tgaaatagaa cgttcaacgt tgccacctat tccaaaagga tttttatctt ctaaacttgc
     1501 ttctacggca cgattaggtg aattctatat tttatttatt tttcaataat ctcagttttt
     1561 cagccaatca catatttgag cttgtatcag tatatggaat ggcgaaaagt cttaatcgga
     1621 aaccagcaat tttcgttgaa gattcaaaat ataatttatt gatcacagga gttcgaaaag
     1681 tattaccggg gctgttagat gaatttcaaa tttttgaata tccagtgagt tttattgatt
     1741 ttcaaaataa gaaaaatttc ttgattgatt tttttaggtg cacaacaaag ccaccaaagt
     1801 tccgttgagt gagaaatgct gtatttttga taatccagat aaatttaaca atatctccag
     1861 cgaatatctt cacttgactg gtcattttta tcaggtaagg gatttaattt ttaatcttta
     1921 aattttgttt ttttcagagt tggaaatatt tcgataaata caaggaaaaa gtgcaaagtt
     1981 ttgtaaaacc agccattgat ttctcgccac ttcccaactc tgatagcagt aatttcatgt
     2041 aaataatttg ttgatgagtt atataaatat tacccttcag ttccagaatt tgcatacaca
     2101 ttcggagaac agattttgtg gacggtcagc atcattcttc aaatgtctcc ttcataaaac
     2161 cagctttgga gtttatcaaa gaacgaggta atttggctgg aaactaaata aaaaaattat
     2221 atatggttca aatttagagc agaaagatgt aaataagaaa atgttgacag ttattatggg
     2281 agatgaccca gattttgaag cgaaaatgtt tgagggtaca gtacgagcaa agaaggaggc
     2341 taagattgaa gaaacggtaa gaagaattcc gaaggaggaa agcacaactt caggtgtacc
     2401 agcctaacac ccactatgct ctccaacaag tttatatatt ttcagacaaa atacttcgtt
     2461 tctgaaaaca ctcctcagga cgatctagca tactcacatt atagttgtga tgcaacttta
     2521 ataactggtt agttttttat cccaatattg ttctaagtca atattaattt tcagctccat
     2581 cttcgacatt tggatggtgg cttggatacc tttcaaaagg acaagctgtt tattatcaag
     2641 atattcgatc tacaaatgat gttaattatg taataactgt atttatacta acaaattttt
     2701 ataagtttta attttcagaa aaatggagta ctcgatcccg atgacttttt cgttccaagc
     2761 tggacatcga taatgttgga cgagaacaag aaagtcgtag ttgttacata gaaataattt
     2821 attaatgtac aaataattat aagagacgtg tagtgattaa ttcaacattg cggttgttca
     2881 attggtatcg gtgctttaaa tctttcaaag ttcaattttt atttcaagtg tcctgttgtc
     2941 aataaataaa attacttttt tttgttcata gagaagataa tataacggtt ttttaaaaat
     3001 aatttcgtgc tctgaaaatt cttttatcat ttccgcaaga aatgatcaaa atgacaaacc
     3061 tcagaaacca accatcaact gttttttcat gtaataaaac acaggttgcc tacggattca
     3121 taaagttgaa aaaaaaaagt tataattgtt tcctaccgta taaatattcg tgaaatcgtt
     3181 gttcgaagca taagaagctt atcttctctt ccacaattat gatcgtttat tctgtattca
     3241 tcttcgctgt tttggcaatt tcatcggtta ctggaatctt cttaccagga ggaggaggca
     3301 agaagtgtgg aggacatggt gaatatggaa gtggagtcat cattggagcc gaacgtccaa
     3361 agaagtaaat taatttggtt taattaataa atacttttgc gcatcgaata attgctgact
     3421 tcaaccttat cagagaactg accctacagt ggataattta tcaacacaaa taattccgtt
     3481 tcttaaatta taaaatccaa aaaataacaa tagcattcat ataaattaga aacatttcga
     3541 aactatgaaa gtcgaagtca ctgtttttaa ggaagtataa gaattaagaa actattagaa
     3601 atacagaaaa caaagctcag aatttaaaga agtatcaatt ccttacatgg ttttcatgat
     3661 tgttttcatg ttttcatgat tgttttattc atgagaattt tccctgaaca atgaccaaaa
     3721 tttcttaaca aatatgtaat ttatttacac atctgttttc ccaatgagaa gagtggttgg
     3781 atgattgatc atctaacaca agggaatggg ttttgaaaaa ctgtagtttt tacggaccgg
     3841 gcccaggccg ggacccagag ctgatgaaac agtatggatc atctataatc gaccagcatc
     3901 taggttagtc gtcttatcct ggaacaaagt cgatagttgt ggcaaacaat tttggttttt
     3961 ttgcttcaaa cagcaaattc aaagacaccg ttgttccaga ttagaattca gctgaattat
     4021 gcagcttttc aacgactcta aattttttta agatatttca gagtaatttc atattctcct
     4081 aaatgtgcat attttgaaat gctttgttta ttgaattaaa aagcaaaatt atttctttgg
     4141 ccgttcagct ccaatgatga ctccacttcc ataaccacca ccataacctc cacacttctt
     4201 gcctcctcca cctggaaaga agattccaga agcaaatgaa attgccaaaa cagcgaaaat
     4261 gaatacagaa tgaacgttca tagttgttga agagaagatg agcttcttat gatttgaaca
     4321 acaactatat ccgatattta tactaaaata ttatcatgaa atcgtaggca aaatcaagtt
     4381 ttattacgtt cttatcaatg gttgaggttt gtgatttgaa tcacctttgt gtttgcaaaa
     4441 atgaaaaacg ttacttgatt atttgctctt ttgttttatc gatatctgac gtgatgattg
     4501 tcacactcaa catcagaaac ttcgggcaaa actgtaacaa gcagtttgca atttaaactt
     4561 gtaataaaac attttatcga aaactgtaag tatttattgt ggcctacact tcaataaaat
     4621 ttggataaaa atgttatgac agtttcctac cgtataaata ttcatgaaat cgttgttcga
     4681 agcataagaa gctcatcttc tcttcatcaa ctatgaacgt ttattctgta ttcatcttcg
     4741 ctatattggc aatttcatcg gcttctggaa tcttcctacc tggaggcgga ggcaagaagt
     4801 gtggaggata tgggggtgga tatggaagtg gagtcatcat tggagctgaa cgtccaaaga
     4861 aataaatgaa tttgtttatt gaataaatac tattgagcat cgaatatttg ttctgaaaag
     4921 atcacatcgt aaatttaaaa cactgttttc tcacttttta atggaaaata ttcaattctt
     4981 tgaaacttcg tggaagctga aaagtatcag aacacaaact ctaaattttc ctgggggctt
     5041 ctcccgcaac tatgaaaata taaacttcca taacttgttc attccaaacc ttatttcatc
     5101 aaattcagtc aaaagaggtt ccttttcatc cgctcgtccg tgtcccgaaa atgaagatgt
     5161 gctgccctcc catccaagtg tctctccctg tgtatgggtg ggggacgttg attggccatt
     5221 tctgtctacg tctcttcttc tcatacacta ttcaaatgct cagaggtgag tctgaaatgc
     5281 aatttagggt ttgcgagtgc tcggctccct ccgctcctac tctttacaca cttggctgcg
     5341 ggcggacgcg aaggtcctcc aatttttctt cgtctcggtc acttcacttc gtactttttc
     5401 aatttcgccg aaataagact attcttcctt agctgacttc tttcttgtac tctcaccctt
     5461 tctaagacat gtgttaatcc gaaagaacat ccaattttct gtgtacaatg aagtgaagga
     5521 tcaaatttcc gagaagaaag gcaaagcaag tcgactagag gatcccccgg gccatggtga
     5581 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5641 taaacggcca caggttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     5701 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5761 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     5821 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgtaccatc ttcttcaagg
     5881 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     5941 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6001 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     6061 aggcccactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     6121 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6181 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6241 agttcgtgac cgccgcccgc atgcatgacc aactgacaga agagcagatt gcagagttca
     6301 aagaagcctt ctcattattc gacaaggatg gggacggcac catcaccaca aaggaacttg
     6361 gcaccgttat gaggtcgctt ggacaaaacc caacggaagc agaattgcag gatatgatca
     6421 atgaagtcga tgctgatggc aatggaacga tttactttcc tgaatttctt actatgatgg
     6481 ctagaaaaat gaaggacaca gacagcgaag aggaaatccg agaagcattc cgtgtttttg
     6541 acaaggatgg gaacggctac atcagcgctg ctcagttacg tcacgtcatg acaaacctcg
     6601 gggagaagtt aacagatgaa gaagttgatg aaatgataag ggaagcagat atcgatggtg
     6661 atggccaagt aaactatgaa gagtttgtac aaatgatgac agcaaagggg gggaagaggc
     6721 gctggaagaa aaacttcatt gccgtcagcg ctgccaaccg gttcaagaag atctccagct
     6781 ccggggcact ggagctcatg gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     6841 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     6901 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     6961 ccgccgggat cactctcggc atggacgagc tgtacaaggg tggcagcggt ggcatggtga
     7021 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7081 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7141 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     7201 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     7261 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     7321 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     7381 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     7441 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     7501 aggccaactt caagatccgc cacaacatcg agtaagaatt ggaattcgat atgcagaagc
     7561 tgatctcaga ggaggacctg cttcagaagc ttatctcaga ggaggacctt cgacctcgag
     7621 ggggggcccg gtacctatgg tgaagggcga ggagctgttc accggggtgg tgcccatcct
     7681 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     7741 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     7801 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc
     7861 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     7921 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     7981 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     8041 catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga
     8101 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
     8161 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     8221 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     8281 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggatga
     8341 gctgtacaag taggagctcc gcatcggccg ctgtcatcag atcgccatct cgcgcccgtg
     8401 cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct
     8461 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt
     8521 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
     8581 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     8641 acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat
     8701 catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca
     8761 atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa
     8821 agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct
     8881 gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct
     8941 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt
     9001 tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg
     9061 gtttttgcct taaatgacag aatacattcc caatatacca aacataactg tttctactag
     9121 tggccacgtg ggccgtgcac cttaagcttg gcactggccg tcgttttaca acgtcgtgac
     9181 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9241 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9301 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     9361 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     9601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9721 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     9841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     9901 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     9961 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    10021 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    10081 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    10501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    10561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    10621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    10681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    10741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11401 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    11461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    11521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    11581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    11641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    11701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    11761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    11881 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    11941 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    12001 agtcagtgag cgaggaagcg gaaga
//
