LOCUS       sequence-351478-        8768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT template plasmid for making ABE8e-P2A-GFP modRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Haideri T, Howells A, Jiang Y, Yang J, Bao X, Lian XL
  TITLE     Robust genome editing via modRNA-based Cas9 or base editor in human 
            pluripotent stem cells.
  JOURNAL   Cell Rep Methods. 2022 Sep 7;2(9):100290. doi: 
            10.1016/j.crmeth.2022.100290. eCollection 2022 Sep 19.
  PUBMED    36160051
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-09-7"; volume: "2(9):100290. doi"; pages: " 
            10.1016/j.crmeth.2022.100290. eCollection 2022 Sep 19"
COMMENT     Sequence Label: modRNAc0-ABE8e-P2A-GFP
FEATURES             Location/Qualifiers
     source          1..8768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..173
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(480..536)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(552..572)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(615..4688,4689..4691,4692..4715))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(615..4715)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(5310..5330)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5462..5481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5463..5481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5488..5505)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5488..5504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5497..5519)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5645..6100
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5732..5751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5942..5963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6178..6282
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6283..6351,6352..7143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6501..6520)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7314..7902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7803..7822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8056..8073
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8190..8211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8226..8231,8232..8249,8250..8256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8264..8280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8269..8291
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8288..8304
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8288..8304
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8322..8340
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8322..8339
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(8510..8530)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(8531..8768,1..473,474..476,477..479))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8531..8768,1..473,474..476,477..479))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8573..8594)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
       61 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      121 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      181 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
      241 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
      301 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      361 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      421 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatt
      481 ggcccgggat tctcttcgac atcccctgct tgtttcaaca gggagaagtt agtggcgccg
      541 ctgccgctag cgactttcct cttcttcttg ggctcgaatt cgctgccgtc ggcggttctt
      601 tttgagccgc cagagtcacc tcccagctga gacaggtcga tccgtgtctc gtacaggccg
      661 gtgatgctct ggtggatcag ggtggcgtcc agcacctctt tggtgctggt gtacctcttc
      721 cggtcgatgg tggtgtcaaa gtacttgaag gcggcagggg ctcccagatt ggtcagggta
      781 aacaggtgga tgatattctc ggcctgctct ctgatgggct tatcccggtg cttgttgtag
      841 gcggacagca ctttgtccag attagcgtcg gccaggatca ctctcttgga gaactcgctg
      901 atctgctcga tgatctcgtc caggtagtgc ttgtgctgtt ccacaaacag ctgtttctgc
      961 tcattatcct cgggggagcc cttcagcttc tcatagtggc tggccaggta caggaagttc
     1021 acatatttgg agggcagggc cagttcgttt cccttctgca gttcgccggc agaggccagc
     1081 attctcttcc ggccgttttc cagctcgaac agggagtact taggcagctt gatgatcagg
     1141 tcctttttca cttctttgta gcccttggct tccagaaagt cgatgggatt cttctcgaag
     1201 ctgcttcttt ccatgatggt gatccccagc agctctttca cactcttcag tttcttggac
     1261 ttgccctttt ccactttggc caccaccagc acagaatagg ccacggtggg gctgtcgaag
     1321 ccgccgtact tcttagggtc ccagtccttc tttctggcga tcagcttatc gctgttcctc
     1381 ttgggcagga tagactcttt gctgaagccg cctgtctgca cctcggtctt tttcacgata
     1441 ttcacttggg gcatgctcag cactttccgc acggtggcaa aatcccggcc cttatcccac
     1501 acgatctccc cggtttcgcc gtttgtctcg atcagaggcc gcttccggat ctcgccgttg
     1561 gccagggtaa tctcggtctt gaaaaagttc atgatgttgc tgtagaagaa gtacttggcg
     1621 gtagccttgc cgatttcctg ctcgctcttg gcgatcatct tccgcacgtc gtacaccttg
     1681 tagtcgccgt acacgaactc gctttccagc ttagggtact ttttgatcag ggcggttccc
     1741 acgacggcgt tcaggtaggc gtcgtgggcg tggtggtagt tgttgatctc gcgcactttg
     1801 taaaactgga aatccttccg gaaatcggac accagcttgg acttcagggt gatcactttc
     1861 acttcccgga tcagcttgtc attctcgtcg tacttagtgt tcatccggga gtccaggatc
     1921 tgtgccacgt gctttgtgat ctgccgggtt tccaccagct gtctcttgat gaagccggcc
     1981 ttatccagtt cgctcaggcc gcctctctcg gccttggtca gattgtcgaa ctttctctgg
     2041 gtaatcagct tggcgttcag cagctgccgc cagtagttct tcatcttctt cacgacctct
     2101 tcggagggca cgttgtcgct cttgccccgg ttcttgtcgc ttctggtcag caccttgttg
     2161 tcgatggagt cgtccttcag aaagctctga ggcacgatat ggtccacatc gtagtcggac
     2221 agccggttga tgtccagttc ctggtccacg tacatatccc gcccattctg caggtagtac
     2281 aggtacagct tctcgttctg cagctgggtg ttttccacgg ggtgttcttt caggatctgg
     2341 ctgcccagct ctttgatgcc ctcttcgatc cgcttcattc tctcgcggct gttcttctgt
     2401 cccttctggg tggtctggtt ctctctggcc atttcgatca cgatgttctc gggcttgtgc
     2461 cggcccatca ctttcacgag ctcgtccacc accttcactg tctgcaggat gcccttctta
     2521 atggcggggc tgccggccag attggcaatg tgctcgtgca ggctatcgcc ctggccggac
     2581 acctgggctt tctggatgtc ctctttaaag gtcaggctgt cgtcgtggat cagctgcatg
     2641 aagtttctgt tggcgaagcc gtcggacttc aggaaatcca ggattgtctt gccggactgc
     2701 ttgtcccgga tgccgttgat cagcttccgg ctcagcctgc cccagccggt gtatctccgc
     2761 cgcttcagct gcttcatcac tttgtcgtcg aacaggtggg cataggtttt cagccgttcc
     2821 tcgatcatct ctctgtcctc aaacagtgtc agggtcagca cgatatcttc cagaatgtcc
     2881 tcgttttcct cattgtccag gaagtccttg tccttgataa ttttcagcag atcgtggtat
     2941 gtgcccaggg aggcgttgaa ccgatcttcc acgccggaga tttccacgga gtcgaagcac
     3001 tcgattttct tgaagtagtc ctctttcagc tgcttcacgg tcactttccg gttggtcttg
     3061 aacagcaggt ccacgatggc ctttttctgc tcgccgctca ggaaggcggg ctttctcatt
     3121 ccctcggtca cgtatttcac tttggtcagc tcgttataca cggtgaagta ctcgtacagc
     3181 aggctgtgct tgggcagcac cttctcgttg ggcaggttct tatcgaagtt ggtcatccgc
     3241 tcgatgaagc tctgggcgga agcgcccttg tccaccactt cctcgaagtt ccagggggtg
     3301 atggtttcct cgctctttct ggtcatccag gcgaatctgc tgtttcccct ggccagaggg
     3361 cccacgtagt aggggatgcg gaaggtcagg atcttctcga tcttttcccg gttgtccttc
     3421 aggaatgggt aaaaatcttc ctgccgccgc agaatggcgt gcagctctcc caggtggatc
     3481 tggtggggga tgctgccgtt gtcgaaggtc cgctgcttcc gcagcaggtc ctctctgttc
     3541 agcttcacga gcagttcctc ggtgccgtcc atcttttcca ggatgggctt gatgaacttg
     3601 tagaactctt cctggctggc tccgccgtca atgtagccgg cgtagccgtt cttgctctgg
     3661 tcgaagaaaa tctctttgta cttctcaggc agctgctgcc gcacgagagc tttcagcagg
     3721 gtcaggtcct ggtggtgctc gtcgtatctc ttgatcatag aggcgctcag gggggccttg
     3781 gtgatctcgg tgttcactct caggatgtcg ctcagcagga tggcgtcgga caggttcttg
     3841 gcggccagaa acaggtcggc gtactggtcg ccgatctggg ccagcaggtt gtccaggtcg
     3901 tcgtcgtagg tgtccttgct cagctgcagt ttggcatcct cggccaggtc gaagttgctc
     3961 ttgaagttgg gggtcaggcc caggctcagg gcaatcaggt ttccgaacag gccattcttc
     4021 ttctcgccgg gcagctgggc gatcagattt tccagccgtc tgctcttgct cagtctggca
     4081 gacaggatgg ccttggcgtc cacgccgctg gcgttgatgg ggttttcctc gaacagctgg
     4141 ttgtaggtct gcaccagctg gatgaacagc ttgtccacgt cgctgttgtc ggggttcagg
     4201 tcgccctcga tcaggaagtg gccccggaac ttgatcatgt gggccagggc cagatagatc
     4261 agccgcaggt cggccttgtc ggtgctgtcc accagtttct ttctcaggtg gtagatggtg
     4321 gggtacttct cgtggtaggc cacctcgtcc acgatgttgc cgaagatggg gtgccgctcg
     4381 tgcttcttat cctcttccac caggaaggac tcttccagtc tgtggaagaa gctgtcgtcc
     4441 accttggcca tctcgttgct gaagatctct tgcagatagc agatccggtt cttccgtctg
     4501 gtgtatcttc ttctggcggt tctcttcagc cgggtggcct cggctgtttc gccgctgtcg
     4561 aacagcaggg ctccgatcag gttcttcttg atgctgtgcc ggtcggtgtt gcccagcacc
     4621 ttgaatttct tgctgggcac cttgtactcg tcggtgatca cggcccagcc cacagagttg
     4681 gtgccgatgg ccaggccgat gctgtacttc ttgtctgacc ccccgctgct gcccccgctg
     4741 ctttcaggtg ttgcgctctc gcttgtgcca ggtgtctcag agccagagga gcctccgcta
     4801 gatcctccgg agttgatgga gctctgggcc ttcttctgag cattgaacac ctgtctaggc
     4861 atccgataga aatcgcacag cagggcggca cattcatctg ccaggattcc ctcggtaatt
     4921 tcgacgcggt gattcatgcc ggggtagttc agcacgttca tcagggagcc tgcggcgcct
     4981 cttttagaat ttctccatcc aaacaccacg cggccgatcc tagagtggat catggcgccg
     5041 gcgcacatca cgcaaggctc gaatgtcacg tacagggtgg cgtcaatcag tctgtagttc
     5101 tgcatgacca ggccgccctg tctcagggcc ataatttcgg catgggctgt tgggtcgtgc
     5161 aggccgatgg ctctgttcca gccctcgccg atcactctat tgttcagcac cagcacggct
     5221 cccacaggca cctccctctc atcccgtgcc ctcttggcca gggtcagggc atgtctcatc
     5281 cagtactcgt gggaaaactc cacctcagag actttccgct tcttctttgg tgactcgaac
     5341 tcgcttccgt cggctgtccg tttcatggtg gcgaattcgc atgccatgga tccggtgtct
     5401 gtttgaggtt gctagtgaac acagttgtgt cagaagcaaa tgtgacgtcg ggcccaattc
     5461 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     5521 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5581 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5641 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5701 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5761 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5821 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5881 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5941 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6001 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6061 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     6121 ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc
     6181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8041 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8101 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8161 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8221 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8281 tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga
     8341 atactcaagc tatgcatatg gtcgacgcaa tgaaaataaa tgttttttat taggcagaat
     8401 ccagatgctc aaggcccttc ataatatccc ccagtttagt agttggactt agggaacaaa
     8461 ggaaccttta atagaaattg gacagcaaga aagcgagctc actagtttat accttacgct
     8521 tcttctttgg cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     8581 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     8641 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     8701 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     8761 tgatgccg
//
