LOCUS       sequence-351480-       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for generation of ClonMapper gRNA barcode library for use 
            in 10X feature barcoding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Gardner A, Morgan D, Al'Khafaji A, Brock A
  TITLE     Functionalized Lineage Tracing for the Study and Manipulation of 
            Heterogeneous Cell Populations.
  JOURNAL   Methods Mol Biol. 2022;2394:109-131. doi: 
            10.1007/978-1-0716-1811-0_8.
  PUBMED    35094325
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2394:109-131. doi"; pages: " 
            10.1007/978-1-0716-1811-0_8"
COMMENT     Sequence Label: pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(96..551)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(233..254)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     445..464
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     678..700
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     692..709
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     693..709
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     719..738
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        719..737
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1053..1251
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1206..1226
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1269..1449
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1496..1621
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2120..2353
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2538..2582
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2880..2997
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3072..3320
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3072..3092
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3243..3262
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        5007..5506
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5042..5065)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5439..5458
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5546..6142
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5546..5565)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6042..6062
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6161..6214
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6215..6916
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(6976..6996)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7394..7405)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7857..7874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7863..8087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8113..8133)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8113..8131)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8152..8168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8152..8168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8165..8187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8176..8192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8200..8206,8207..8224,8225..8230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8245..8266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8383..8400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8554..9142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8634..8653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9313..10104,10105..10173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9936..9955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10174..10208,1..70))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
       61 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
      121 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
      181 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
      241 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
      301 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
      361 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
      421 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
      481 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
      541 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      601 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
      661 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
      721 atacgactca ctatagggcg aattgactag tacataactt acggtaaatg gcccgcctgg
      781 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      841 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      901 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      961 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1021 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1081 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1141 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1201 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     1261 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     1321 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     1381 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     1441 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1501 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1561 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1621 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1681 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1741 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1801 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1861 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1921 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgcg ctgatcttca
     1981 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     2041 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     2101 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     2161 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     2221 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     2281 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     2341 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     2401 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     2461 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     2521 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     2581 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     2641 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     2701 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     2761 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2821 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     2881 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2941 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     3001 gtttattaca gggacagcag agatccagtt tggttagtac cgggccctac gcgtccaagg
     3061 tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     3121 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
     3181 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     3241 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3301 tgtggaaagg acgaaacacc gggtcttcgg aattcagctc accaaggtca aatacgtcac
     3361 agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta tcgtggacct
     3421 cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagact atttcaaaaa
     3481 gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca acgcatccct
     3541 gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg acaatgagga
     3601 gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag atagggagat
     3661 gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca tgaaacagct
     3721 caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca atgggatccg
     3781 agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat ttgccaaccg
     3841 gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca tccagaaagc
     3901 acaagtttct ggccaggggg acagtcttca cgagcacatc gctaatcttg caggtagccc
     3961 agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat
     4021 gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc aaactaccca
     4081 gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta taaaagaact
     4141 ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga atgagaagct
     4201 ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac tggacatcaa
     4261 tcggctctcc gactacgacg tggctgctat cgtgccccag tcttttctca aagatgattc
     4321 tattgataat aaagtgttga caagatccga taaagctaga gggaagagtg ataacgtccc
     4381 ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga acgccaaact
     4441 gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc tgtctgagtt
     4501 ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca ccaagcacgt
     4561 ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca aactgattcg
     4621 agaggtgaaa gttattactc tgaagtctaa gctggtctca gatttcagaa aggactttca
     4681 gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct acctgaatgc
     4741 agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg
     4801 agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tgaattcagt ggcaagagca
     4861 tcatagaaga agacacgttt aagagctaag ctggaaacag catagcaagt ttaaataagg
     4921 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcgctttaag gccggtccta
     4981 gcaatttttt tggatccaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     5041 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     5101 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     5161 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     5221 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     5281 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     5341 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     5401 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     5461 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct
     5521 agatctcgag cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     5581 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5641 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5701 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5761 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5821 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5881 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5941 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     6001 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     6061 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     6121 catgacccgc aagcccggtg ccggcggcgg gtccggagga gagggcagag gaagtctcct
     6181 aacatgcggt gacgtggagg agaatcctgg cccaatgagc gagctgatta aggagaacat
     6241 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
     6301 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
     6361 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     6421 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     6481 atgggagaga gtcaccacat acgaggacgg gggcgtgctg accgctaccc aggacaccag
     6541 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     6601 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc
     6661 cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
     6721 tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
     6781 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac
     6841 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
     6901 gcacaagctt aattgagcgg ccgctcaacc tctggattac aaaatttgtg aaagattgac
     6961 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7021 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7081 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7141 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7201 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7261 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7321 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7381 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7441 tctgcggcct cttccgcgtc tacgccttcg ccctcagacg agtcggatct ccctttgggc
     7501 cgcctccccg caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7561 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa gtcaagatct
     7621 gctttttgcc tgtactgggt ctctctggtt agaccagagt ctctctggtt agaccagatc
     7681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7801 ctcagaccct tttagtcagt gtggaaaatc tctagcagtt taaacccgct gatcagcctc
     7861 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7921 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7981 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8041 ttgggaagtc aatagcaggc atgctgggga tgcggtgggc tctatgggcg gccgttaatt
     8101 aagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     8161 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat
     8221 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8341 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8401 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8521 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8641 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9241 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9301 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9361 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9421 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9601 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9721 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9781 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10141 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10201 catttatc
//
