LOCUS       sequence-351497-        2670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector for expressing CFL-SN and GCaMP6f.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Goto A, Bota A, Miya K, Wang J, Tsukamoto S, Jiang X, Hirai D, 
            Murayama M, Matsuda T, McHugh TJ, Nagai T, Hayashi Y
  TITLE     Stepwise synaptic plasticity events drive the early phase of memory 
            consolidation.
  JOURNAL   Science. 2021 Nov 12;374(6569):857-863. doi: 
            10.1126/science.abj9195. Epub 2021 Nov 11.
  PUBMED    34762472
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abj9195"; journalName: "Science"; date: 
            "2021-11-12- 12"; volume: "374"; issue: "6569"; pages: "857-863"
COMMENT     Sequence Label: pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..2670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(505..507,508..513,514..1221)
                     /codon_start=1
                     /product="monomeric variant of the photosensitizing red 
                     fluorescent protein KillerRed (Takemoto et al., 2013)"
                     /label=SuperNova
                     /translation="MGSEVGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
                     VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
                     TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPSETHMFPHGPNAVRQTA
                     TIGFTTADGGKMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQTRDTSDKRDHVCQREVA
                     YAHSVPRITSAIGSDED"
     CDS             1237..1293
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1318..1326,1327..1344,1345..1347,1348..1380,1381..1383,
                     1384..1407,1408..1434,1435..1491,1492..1497,1498..1767,
                     1768..1785,1786..2220,2221..2226,2227..2670)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      1414..1423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1704..1725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1831..1852)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2092..2111)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggcctccg gtgtggctgt ctctgatggt gtcatcaagg tgttcaacga catgaaggtg
       61 cgtaagtctt caacgccaga ggaggtgaag aagcgcaaga aggcggtgct cttctgcctg
      121 agtgaggaca agaagaacat catcctggag gagggcaagg agatcctggt gggcgatgtg
      181 ggccagactg tcgacgatcc ctacgccacc tttgtcaaga tgctgccaga taaggactgc
      241 cgctatgccc tctatgatgc aacctatgag accaaggaga gcaagaagga ggatctggtg
      301 tttatcttct gggcccccga gtctgcgccc cttaagagca aaatgattta tgccagctcc
      361 aaggacgcca tcaagaagaa gctgacaggg atcaagcatg aattgcaagc aaactgctac
      421 gaggaggtca aggaccgctg caccctggca gagaagctgg ggggcagtgc ggtcatctcc
      481 ctggagggca agcctttgtc tagaatgggt tcagaggtcg gccccgccct gttccagagc
      541 gacatgacct tcaaaatctt catcgacggc gaggtgaacg gccagaagtt caccatcgtg
      601 gccgacggca gcagcaagtt cccccacggc gacttcaacg tgcacgccgt gtgcgagacc
      661 ggcaagctgc ccatgagctg gaagcccatc tgccacctga tccagtacgg cgagcccttc
      721 ttcgcccgct accccgacgg catcagccat ttcgcccagg agtgcttccc cgagggcctg
      781 agcatcgacc gcaccgtgcg cttcgagaac gacggcacca tgaccagcca ccacacctac
      841 gagctggacg acacctgcgt ggtgagccgc atcaccgtga actgcgacgg cttccagccc
      901 gacggcccca tcatgcgcga ccagctggtg gacatcctgc ccagcgagac ccacatgttc
      961 ccccacggcc ccaacgccgt gcgccagacc gccaccatcg gcttcaccac cgccgacggc
     1021 ggcaagatga tgggccactt cgacagcaag atgaccttca acggcagccg cgccatcgag
     1081 atccccggcc cacacttcgt gaccatcatc accaagcaga cgagggacac tagcgacaag
     1141 cgcgaccacg tgtgccagcg cgaggtggcc tacgcccaca gcgtgccccg catcaccagc
     1201 gccatcggta gcgacgagga tctcgaggga agcggagcca ctaacttctc cctgttgaaa
     1261 caagcaggtg acgtcgagga gaatcctggc ccaggatccc gggatccacc ggtcaccatg
     1321 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
     1381 cgggatctgt acgacgatga cgataaggat ctcgccacca tggtcgactc atcacgtcgt
     1441 aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc actcgagaac
     1501 gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     1561 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
     1621 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actttcgaaa
     1681 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1741 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
     1801 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1861 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
     1921 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1981 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2041 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
     2101 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2161 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2221 ctgccggacc aactgactga agagcagatc gcagaattta aagaggaatt ctccctattt
     2281 gacaaggacg gggatgggac aataacaacc aaggagctgg ggacggtgat gcggtctctg
     2341 gggcagaacc ccacagaagc agagctgcag gacatgatca atgaagtaga tgccgacggt
     2401 gacggcacaa tcgacttccc tgagttcctg acaatgatgg caagaaaaat gaaatacagg
     2461 gacacggaag aagaaattag agaagcgttc ggtgtgtttg ataaggatgg caatggctac
     2521 atcagtgcag cagagcttcg ccacgtgatg acaaaccttg gagagaagtt aacagatgaa
     2581 gaggttgatg aaatgatcag ggaagcagac atcgatgggg atggtcaggt aaactacgaa
     2641 gagtttgtac aaatgatgac agcgaagtga
//
