LOCUS       sequence-351498-        9334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human FOXA1 with C-terminal V5 tag, puromycin 
            selection marker, 3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas 
            P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, 
            Kantoff PW, Brown M, Loda M, Long HW, Freedman ML
  TITLE     The androgen receptor cistrome is extensively reprogrammed in human 
            prostate tumorigenesis.
  JOURNAL   Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 
            Oct 12.
  PUBMED    26457646
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3419"; 
            journalName: "Nat Genet"; date: "2015-11"; volume: "47"; issue: 
            "11"; pages: "1346-51"
COMMENT     Sequence Label: pLX302_FOXA1-V5
FEATURES             Location/Qualifiers
     source          1..9334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    133..154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(169..174,175..192,193..199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     212..234
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     231..247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     231..247
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     266..286
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        268..286
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        314..540
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             541..721
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    768..893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1386..1619
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1804..1848
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2023..2523
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2442..2460
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2545..3144
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2545..2564)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3041..3061
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3186..3303
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3420..3723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3724..3927
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3878..3898
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3950..3974
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5391..5415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5417..5458
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5500..6088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5553..5573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5971..5982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6160..6393
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6471..6592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6508..6527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6562..6581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6632..6767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6787..6806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6788..6806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6816..6833)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6816..6832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6825..6847)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6974..7429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7061..7080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7271..7292
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7455..7559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7560..7628,7629..8420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7778..7797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8591..9179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9080..9099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9333..9334,1..16)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      241 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
      301 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
      361 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
      421 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
      481 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
      541 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      601 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      661 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      721 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      781 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      841 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      901 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      961 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1021 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1081 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1141 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1201 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     1261 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1321 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1381 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1441 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1501 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1561 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1621 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1681 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1741 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1801 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1861 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1921 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1981 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
     2041 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     2101 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     2161 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     2221 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     2281 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     2341 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     2401 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     2461 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     2521 cagggggtac caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     2581 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     2641 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     2701 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc
     2761 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     2821 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     2881 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     2941 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     3001 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     3061 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     3121 atgacccgca agcccggtgc ctgagaattc tagatcttga gacaaatggc agtattcatc
     3181 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     3241 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     3301 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg
     3361 ggttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     3421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     3541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     3601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     3661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3721 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     3781 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     3841 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     3901 cggtgggagg tctatataag cagagctctc tggctaactg tcgggatcaa caagtttgta
     3961 caaaaaagtt ggcatgttag gaactgtgaa gatggaaggg catgaaacca gcgactggaa
     4021 cagctactac gcagacacgc aggaggccta ctcctccgtc ccggtcagca acatgaactc
     4081 aggcctgggc tccatgaact ccatgaacac ctacatgacc atgaacacca tgactacgag
     4141 cggcaacatg accccggcgt ccttcaacat gtcctatgcc aacccgggcc taggggccgg
     4201 cctgagtccc ggcgcagtag ccggcatgcc ggggggctcg gcgggcgcca tgaacagcat
     4261 gactgcggcc ggcgtgacgg ccatgggtac ggcgctgagc ccgagcggca tgggcgccat
     4321 gggtgcgcag caggcggcct ccatgaatgg cctgggcccc tacgcggccg ccatgaaccc
     4381 gtgcatgagc cccatggcgt acgcgccgtc caacctgggc cgcagccgcg cgggcggcgg
     4441 cggcgacgcc aagacgttca agcgcagcta cccgcacgcc aagccgccct actcgtacat
     4501 ctcgctcatc accatggcca tccagcaggc gcccagcaag atgctcacgc tgagcgagat
     4561 ctaccagtgg atcatggacc tcttccccta ttaccggcag aaccagcagc gctggcagaa
     4621 ctccatccgc cactcgctgt ccttcaatga ctgcttcgtc aaggtggcac gctccccgga
     4681 caagccgggc aagggctcct actggacgct gcacccggac tccggcaaca tgttcgagaa
     4741 cggctgctac ttgcgccgcc agaagcgctt caagtgcgag aagcagccgg gggccggcgg
     4801 cgggggcggg agcggaagcg ggggcagcgg cgccaagggc ggccctgaga gccgcaagga
     4861 cccctctggc gcctctaacc ccagcgccga ctcgcccctc catcggggtg tgcacgggaa
     4921 gaccggccag ctagagggcg cgccggcccc cgggcccgcc gccagccccc agactctgga
     4981 ccacagtggg gcgacggcga cagggggcgc ctcggagttg aagactccag cctcctcaac
     5041 tgcgcccccc ataagctccg ggcccggggc gctggcctct gtgcccgcct ctcacccggc
     5101 acacggcttg gcaccccacg agtcccagct gcacctgaaa ggggaccccc actactcctt
     5161 caaccacccg ttctccatca acaacctcat gtcctcctcg gagcagcagc ataagctgga
     5221 cttcaaggca tacgaacagg cactgcaata ctcgccttac ggctctacgt tgcccgccag
     5281 cctgcctcta ggcagcgcct cggtgaccac caggagcccc atcgagccct cagccctgga
     5341 gccggcgtac taccaaggtg tgtattccag acccgtccta aacacttcct acccaacttt
     5401 cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta
     5461 gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggattacaaa
     5521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5881 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     5941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6061 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     6121 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6181 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6241 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6301 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6361 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     6421 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6481 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6541 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6601 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6721 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     6781 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     6841 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6901 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6961 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7021 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7201 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7261 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7321 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7381 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     7441 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7501 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7561 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7621 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7681 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7741 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7801 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7861 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7921 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7981 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8041 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8101 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8161 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8221 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8281 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8341 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8401 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8461 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8521 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8581 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8641 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8701 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8761 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8821 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8881 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8941 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9001 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9061 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9121 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9181 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9241 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9301 ataccgctcg ccgcagccga acgaccgagc gcag
//
