LOCUS       sequence-351503-        8435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KSHV ORF22 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0178
FEATURES             Location/Qualifiers
     source          1..8435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    914..938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3153..3218
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3248..3272)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3283..3285,3286..3288,3289..4002)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3316..3344)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3939..3959
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4024..4041)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4030..4254
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4300..4728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4387..4406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4597..4618
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4737..4757)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4742..5071
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4922..5057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4984..5003
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5138..5932
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5192..5211)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5802..5821
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6108..6229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6145..6164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6199..6218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6278..6294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6278..6294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6291..6313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6302..6318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6326..6332,6333..6350,6351..6356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6371..6392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6509..6526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6680..7268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6760..6779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7439..8230,8231..8299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8062..8081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8300..8404)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttgat caaacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcgcca
      961 ccatgcaggg tctagccttc ttggcggccc ttgcatgctg gcgatgcata tcgttgacat
     1021 gtggagccac tggcgcgttg ccgacaacgg cgacgacaat aacccgctcc gccacgcagc
     1081 tcatcaatgg gagaaccaac ctctccatag aactggaatt caacggcact agtttttttc
     1141 taaattggca aaatctgttg aatgtgatca cggagccggc cctgacagag ttgtggacct
     1201 ccgccgaagt cgccgaggac ctcagggtaa ctctgaaaaa gaggcaaagt ctttttttcc
     1261 ccaacaagac agttgtgatc tctggagacg gccatcgcta tacgtgcgag gtgccgacgt
     1321 cgtcgcaaac ttataacatc accaagggct ttaactatag cgctctgccc gggcaccttg
     1381 gcggatttgg gatcaacgcg cgtctggtac tgggtgatat cttcgcatca aaatggtcgc
     1441 tattcgcgag ggacacccca gagtatcggg tgttttaccc aatgaatgtc atggccgtca
     1501 agttttccat atccattggc aacaacgagt ccggcgtagc gctctatgga gtggtgtcgg
     1561 aagatttcgt ggtcgtcacg ctccacaaca ggtccaaaga ggctaacgag acggcgtccc
     1621 atcttctgtt cggtctcccg gattcactgc catctctgaa gggccatgcc acctatgatg
     1681 aactcacgtt cgcccgaaac gcaaaatatg cgctagtggc gatcctgcct aaagattctt
     1741 accagacact ccttacagag aattacactc gcatatttct gaacatgacg gagtcgacgc
     1801 ccctcgagtt cacgcggacg atccagacca ggatcgtatc aatcgaggcc aggcgcgcct
     1861 gcgcagctca agaggcggcg ccggacatat tcttggtgtt gtttcagatg ttggtggcac
     1921 actttcttgt tgcgcggggc attgccgagc accgatttgt ggaggtggac tgcgtgtgtc
     1981 ggcagtatgc ggaactgtat tttctccgcc gcatctcgcg tctgtgcatg cccacgttca
     2041 ccactgtcgg gtataaccac accacccttg gcgctgtggc cgccacacaa atagctcgcg
     2101 tgtccgccac gaagttggcc agtttgcccc gctcttccca ggaaacagtg ctggccatgg
     2161 tccagcttgg cgcccgtgat ggcgccgtcc cttcctccat tctggagggc attgctatgg
     2221 tcgtcgaaca tatgtatacc gcctacactt atgtgtacac actcggcgat actgaaagaa
     2281 aattaatgtt ggacatacac acggtcctca ccgacagctg cccgcccaaa gactccggag
     2341 tatcagaaaa gctactgaga acatatttga tgttcacatc aatgtgtacc aacatagagc
     2401 tgggcgaaat gatcgcccgc ttttccaaac cggacagcct taacatctat agggcattct
     2461 ccccctgctt tctaggacta aggtacgatt tgcatccagc caagttgcgc gccgaggcgc
     2521 cgcagtcgtc cgctctgacg cggactgccg ttgccagagg aacatcggga ttcgcagaat
     2581 tgctccacgc gctgcacctc gatagcttaa atttaattcc ggcgattaac tgttcaaaga
     2641 ttacagccga caagataata gctacggtac ccttgcctca cgtcacgtat atcatcagtt
     2701 ccgaagcact ctcgaacgct gttgtctacg aggtgtcgga gatcttcctc aagagtgcca
     2761 tgtttatatc tgctatcaaa cccgattgct ccggctttaa cttttctcag attgataggc
     2821 acattcccat agtctacaac atcagcacac caagaagagg ttgccccctt tgtgactctg
     2881 taatcatgag ctacgatgag agcgatggcc tgcagtctct catgtatgtc actaatgaaa
     2941 gggtgcagac caacctcttt ttagataagt cacctttctt tgataataac aacctacaca
     3001 ttcattattt gtggctgagg gacaacggga ccgtagtgga gataaggggc atgtatagaa
     3061 gacgcgcagc cagtgctttg tttctaattc tctcttttat tgggttctcg ggggttatct
     3121 actttcttta cagactgttt tccatccttt atgactacaa agaccatgac ggtgattata
     3181 aagatcatga tatcgattac aaggatgacg atgacaagta gtaagcggcc gcactcgaga
     3241 tatctagacc cagctttctt gtacaaagtg gttcgatcta gaatggctag caaaggagaa
     3301 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     3361 aaattttctg tcagtggaga gggtgaaggt gatgctacat acggaaagct tacccttaaa
     3421 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttctct
     3481 tatggtgttc aatgcttttc ccgttatccg gatcatatga aacggcatga ctttttcaag
     3541 agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac
     3601 tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta
     3661 aaaggtattg attttaaaga agatggaaac attctcggac acaaactcga gtacaactat
     3721 aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc
     3781 aaaattcgcc acaacattga agatggatcc gttcaactag cagaccatta tcaacaaaat
     3841 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct
     3901 gccctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact
     3961 gctgctggga ttacacatgg catggatgag ctctacaaat aatgaattaa acccgctgat
     4021 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4081 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4141 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4201 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4261 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4321 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4381 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4441 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4501 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4561 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4621 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4681 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     4741 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4801 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4861 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4981 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     5041 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     5101 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5161 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5221 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5281 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     5341 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5401 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5461 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5521 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5581 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5641 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     5701 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5761 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5821 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5881 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5941 ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6001 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6061 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6241 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7081 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8401 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
