LOCUS       sequence-35154-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Wang KC, Yang YW, Liu B, Sanyal A, Corces-Zimmerman R, Chen Y, 
            Lajoie BR, Protacio A, Flynn RA, Gupta RA, Wysocka J, Lei M, Dekker 
            J, Helms JA, Chang HY
  TITLE     A long noncoding RNA maintains active chromatin to coordinate 
            homeotic gene expression.
  JOURNAL   Nature. 2011 Apr 7;472(7341):120-4. doi: 10.1038/nature09819. Epub 
            2011 Mar 20.
  PUBMED    21423168
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature09819"; journalName: "Nature"; date: "2011-04-7- 7"; 
            volume: "472"; issue: "7341"; pages: "120-4"
COMMENT     Sequence Label: BoxB in pcDNA3
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    151..169
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    195..213
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    219..237
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    278..296
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    302..320
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     promoter        complement(438..456)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(439..456)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(476..493)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    482..706
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ctaagctcgc tttcttgctg tccaatttct attaaaggtt cctttgttcc
      121 ctaagtccaa ctactaaact ggggattcct gggccctgaa gaagggcccc tcgactaagt
      181 ccaactacta aactgggccc tgaagaaggg cccatatagg gccctgaaga agggccctat
      241 cgaggatatt atctcgacta agtccaacta ctaaactggg ccctgaagaa gggcccatat
      301 agggccctga agaagggccc tatcgaggat attatctcga ggatattatg aagggcgcga
      361 cggccgccag tgtgctggaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg
      421 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
      481 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      541 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
      601 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
      661 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
      721 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
      781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
//
