LOCUS       sequence-351547-        2106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor-responsive promoter FUS1 of S. cerevisiae (response 
            part).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK002
FEATURES             Location/Qualifiers
     source          1..2106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(488..522)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(593..1252)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1167..1186
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1434..2022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1923..1942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtagtcg gtctcaaacg acgatgattc agttcgcctt ctatcctttg
       61 tttacgtatt tgtttatata tataacttta ttttttttta ttaattgggc tgcaagacaa
      121 ttttgttgtc agtgatgcct caatccttct tttgcttcca tatttaccat gtggaccctt
      181 tcaaaacaga gttgtatctc tgcaggatgc cctttttgac gtattgaatg gcataattgc
      241 actgtcactt ttcgcgctgt ctcattttgg tgcgatgatg aaacaaacat gaaacgtctg
      301 taatttgaaa caaataacgt aattctcggg attggtttta tttaaatgac aatgtaagag
      361 tggctttgta aggtatgtgt tgctcttaaa atatttggat acgacatcct ttatcttttt
      421 tcctttaaga gcaggatata agccatcaag tttctgaaaa tcaaaagatc tatgtgagac
      481 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      541 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
      601 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      661 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      721 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      781 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
      841 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      901 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
      961 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1021 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1081 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1141 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1201 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1261 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1321 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     1381 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1441 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1501 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1561 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1621 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1681 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1741 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1801 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1861 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1921 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1981 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2041 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2101 cctgat
//
