LOCUS       sequence-351554-        2538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor-responsive promoter FUS3 (response part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK003
FEATURES             Location/Qualifiers
     source          1..2538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(915..949)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1020..1679)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1594..1613
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1861..2449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2350..2369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggataaccgt agtcggtctc aaacgaacgt gtgatgacta ctggacactt cagggctttt
       61 ccagatatac agagctttat aatacaatct aagggcaaaa taaaaaagga aaaagagtac
      121 ggtagttgag aaaaaaaccg gaaaggaatt gctttaaaga tcttagtaga atgaaatagt
      181 accacctact cgcctccttt ttttgtgctt cttgagaggt cttttgagct gttgcatcat
      241 gctgttattt agaggttatt ttttgacctt ggtttttcct tcttttcgcc gtactcagcc
      301 cgagaaaaaa gcagcgactg cactaaacta gaggaaagaa ggcttaaaac atcacgcgat
      361 ccatataaag acccgagtag ttggagagat tgactacttg acgttcaaaa gaacatacat
      421 aaggataaat tcgttcggac tttttaactt acacacatct tttggtgttc attcaagggg
      481 cggtagtgct tgtagtccgc acaggcggat actttttagt ttgggaagag ttaactggag
      541 agggcagttt actttcacat actcttttat ctttctgaag taaaatgtga tgtacctaca
      601 agcaatgcat aagctcttga gttttagatc tgtttcaccc tggaactcaa aattctttta
      661 ctcgaaattt ttactttttt tttttttgtt tctgcattct ctcagatttt agatgatgcg
      721 gttttttaca gggcattgaa acaattgcag aaaagcaaca tactaatata tcataacttt
      781 ttactcttgc ctctcagaaa ctatatatac gttgtaatca ttttctttct tctaatagct
      841 agttcgtttg aactacaagg aaataaggca gagaaaaaga aaggaaaata atagatctat
      901 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      961 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1021 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1081 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1141 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1201 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
     1261 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1321 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1381 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1441 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1501 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1561 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1621 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1681 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1741 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     1801 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1861 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1921 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1981 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2041 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2101 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2161 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2221 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2461 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2521 gttatcccct gattctgt
//
