LOCUS       sequence-351557-        2102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor-responsive promoter SST2 (response part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK006
FEATURES             Location/Qualifiers
     source          1..2102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             813..1472
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(879..898)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1543..1577
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgattg atcgggcacg
      721 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt
      781 tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      841 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
      901 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
      961 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     1021 atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     1081 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     1141 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     1201 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtttca gccaatccct
     1261 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     1321 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     1381 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     1441 agtactgcga tgagtggcag ggcggggcgt aatttgatat cgagctcgct tggactcctg
     1501 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc
     1561 cgccgggcgt tttttattgg tctggtctca catagatctc ttacaactct atctttaatt
     1621 accaaattga acctataacg cctcagatag tgcgtgcaaa acctagaagg aacgtacgac
     1681 agtaacacct ccaaagttaa aggacgatga gaagtaaaga cagagctggg attatgattt
     1741 cattcaccgg tataaatata acaggacaga caacaattct aacgcaccag caaccaaatc
     1801 caagtcctag tcaaaaaaaa gcagtattgg gatcattgtt tcaaaattga atattatgaa
     1861 acgtttaaaa cccagacatc ccataattat tgaagatgat tcagttaaaa cttcgtatac
     1921 ttcatggctt cttcattaat agagacaacg gaggtggttc aacttgaact tttccaaagg
     1981 agttgccatc tctttgtatt gttcgaactc tttcaaaaga ccttggtaat cgtttgagac
     2041 cgactacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2101 ca
//
