LOCUS       sequence-351558-        2097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Candida albicans (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK008
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(496..530)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(601..1260)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1175..1194
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1442..2030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1931..1950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgttatc ccctgattct gtggataacc gtagtcggtc tcatatgaaa ttttctttga
       61 ctttgttgac tgctacaatt gctactattg ttgctgctgc acctgctcaa tatactggtc
      121 aagctattga ctcaaatcaa gttgttgaaa ttccagaatc tgctgtggaa gcatatttcc
      181 caattgatga tgaattgacg cctgtttttg gtgaaataga taataaacca gttattttaa
      241 ttgttaatgg tactacttta acttctggtg ctaataatga aaagagagaa gccaaatcta
      301 aaggtggttt cagattgacc aattttggct actttgagcc tggtaaaagg gatgctaacg
      361 ctgatgcagg atttaggttg acaaatttcg gttacttcga acctggaaaa agagatgcca
      421 atgctgaagc tggttttaga ttaacaaact ttggttattt tgaaccaggt aagtaaggat
      481 cctgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
      541 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
      601 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
      661 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      721 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      781 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt
      841 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      901 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      961 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1021 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1081 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1141 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1201 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     1261 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     1321 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc
     1381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1561 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1681 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1741 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1801 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1861 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1921 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1981 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2041 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttc
//
