LOCUS       sequence-351559-        2229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Kluyveromyces lactis (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK011
FEATURES             Location/Qualifiers
     source          1..2229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(660..694)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(765..1424)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1339..1358
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1606..2194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2095..2114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
       61 ccgtagtcgg tctcatatga aattctctac tatattagcc gcatctactg ctttaatttc
      121 cgttgttatg gctgctccag tttctaccga aactgacatc gacgatcttc caatttcggt
      181 tccagaagaa gccttgattg gattcattga cttaaccggg gatgaagttt ccttgttgcc
      241 tgttaataac ggaacccaca ctggtattct attcttaaac accaccatcg ctgaagctgc
      301 tttcgctgac aaggatgatt tgaagaaaag agaagccgat gcttccccat ggagttggat
      361 tactctaaga cctggtcaac caatctttaa aagagaagcc aacgctgacg ctaatgctga
      421 agcatcccca tggagctgga ttactctaag acctggtcaa ccgatcttta agagagaggc
      481 taatgctgat gccaatgcag atgcctcccc atggagctgg atcactctaa gacctggtca
      541 accaatcttt aaaagagaag ccaaccctga ggccgaggct gatgccaaac ctagtgcttg
      601 gagttggatt acattaagac ctggccaacc aattttctga ggatcctgag accagaccaa
      661 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      721 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      961 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
     1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1501 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1741 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2221 gttcctggc
//
