LOCUS       sequence-35156-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Wang KC, Yang YW, Liu B, Sanyal A, Corces-Zimmerman R, Chen Y, 
            Lajoie BR, Protacio A, Flynn RA, Gupta RA, Wysocka J, Lei M, Dekker 
            J, Helms JA, Chang HY
  TITLE     A long noncoding RNA maintains active chromatin to coordinate 
            homeotic gene expression.
  JOURNAL   Nature. 2011 Apr 7;472(7341):120-4. doi: 10.1038/nature09819. Epub 
            2011 Mar 20.
  PUBMED    21423168
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature09819"; journalName: "Nature"; date: "2011-04-7- 7"; 
            volume: "472"; issue: "7341"; pages: "120-4"
COMMENT     Sequence Label: LacZ-5BoxB in pcDNA3.1+
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    158..176
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    202..220
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    226..244
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    285..303
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    309..327
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    401..425
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(507..529)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttagcgagct ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccta agctcgcttt cttgctgtcc aatttctatt aaaggttcct
      121 ttgttcccta agtccaacta ctaaactggg gattcctggg ccctgaagaa gggcccctcg
      181 actaagtcca actactaaac tgggccctga agaagggccc atatagggcc ctgaagaagg
      241 gccctatcga ggatattatc tcgactaagt ccaactacta aactgggccc tgaagaaggg
      301 cccatatagg gccctgaaga agggccctat cgaggatatt atctcgagga tattatgaag
      361 ggcgcgacgg ccgccagtgt gctggaattc tgcagatatc acaagtttgt acaaaaaagc
      421 aggctttaaa ggaaccaatt cagtcgactg gatccactag tccagtgtgg tggaattctg
      481 cagatcgaaa cgatgataga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      541 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      601 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
      661 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
      721 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
      781 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacggg agaatccgac
      841 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
      901 aattattttt
//
