LOCUS       sequence-351560-        2247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Lachancea mirantina (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK014
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             556..567
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(666..700)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(771..1430)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1345..1364
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1612..2200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2101..2120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtagtcggtc
       61 tcatatgaaa ttaactgttt tttggactgc ttttctatca ttcttgacta cttttattgt
      121 tactgcaagt gctgctccag ttgcagattc agcaaatgat ggttctgtta atattccagc
      181 tgaatctatt attggttttt tggatttgtc tgattctaaa gatgctactt tggttcctgt
      241 ttctaatggt actcattctg gtttcttatt tttaaattct acaattttat ctcaagcttc
      301 tactgaaagt ttgaaaaaaa gatctgctca accatggaga tggttgtctt taagagctgg
      361 tgaagctttg cataaaagag aagctgatgc tgaagctgat ccatggcaat ggttatcttt
      421 aagagctggt gaagcattac ataagagaga ggcagaagct gcaccttggt catggttatc
      481 attaagagca ggtgaagctt tacataaaag aaatgctaat gcagaagctg aggcagcacc
      541 atggaggtgg ctgtcattga gggcaggcga agccttacac aaaagggaag ccgatgcagc
      601 tccttggaga tggctatctt tgagagctgg tgaggcttta cattaaggat cctgagacca
      661 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      721 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      781 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      841 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      901 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      961 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     1021 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1081 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1141 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1201 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1261 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1321 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1381 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1441 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     1501 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1741 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2041 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2221 tttacggttc ctggcctttt gctggcc
//
