LOCUS       sequence-351561-        2211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Lachancea thermotolerans (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK015
FEATURES             Location/Qualifiers
     source          1..2211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(595..629)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(700..1359)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1274..1293
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1541..2129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2030..2049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attctgtgga taaccgtagt cggtctcata tgttgaaatc tttctctttg ttcttagcct
       61 ttgctttaac ttctattaca gtttctgctg ctccaattaa tattactgat tctgatccag
      121 ttattccaag agaatctatt tgtggtttct tagatttaac agatgctgaa gatttggcat
      181 tattacctgt ttctaatggt actcattctg gtattttagt tgttaataca actattttag
      241 ctcaagcttt tggttcagat gatattttga caaaaagaga tgcaaatgca gaggcagaag
      301 ctgatccttg gagatggtta tcactttcta gaggccaacc aatgtacaaa agagatgcca
      361 atgccgaagc agatgcagaa ccttggcgtt ggctaagttt gtccagaggt cagccaatgt
      421 ataagagaga tgctaatgct gaagctgatg ctgaaccatg gagatggttg tctttgtcac
      481 gtggtcaacc gatgtataaa cgtgatgcta atgctgaagc cgatgcggag ccatggaggt
      541 ggctgtcttt agcaagagga caacctatgt actaaggatc ctgagaccag accaataaaa
      601 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      661 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      721 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      781 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
      841 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
      901 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
      961 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1021 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1081 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1141 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1201 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1261 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1321 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1381 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1441 ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1501 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1561 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1621 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1681 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1741 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1801 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1861 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1921 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1981 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2041 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2101 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2161 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct g
//
