LOCUS       sequence-351570-        2277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Vanderwaltozyma polyspora (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK018
FEATURES             Location/Qualifiers
     source          1..2277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(657..691)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(762..1421)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1336..1355
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1603..2191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2092..2111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtggataac cgtagtcggt ctcatatgaa attttctact gttttgtcta cagttgcttt
       61 ggctgctact gctgtttctg ctgctccaat ttctagagct tctaatgaaa ctgtggaatc
      121 agtggagtct ggtttgaatg ttccagctga agccgtttta ggttatttgg attttggtga
      181 aaaagatgac gttgcaatgt taccattttc aaacggtact tcaaatggtt tattatttgt
      241 taatactact atttatgatg ccgcttttgc tgattctgat gatgaaagtg catcattagc
      301 aaaaagagat gctgaagctt ggcattggtt gagattaaga tatggtgaac caatttacaa
      361 aagagaggat agtgaaggtg ttgaaaagag ggaagcagct gcagaaccat ggcactggct
      421 aagattgaga tacggagaac ctatttataa acgtgaagat tcagaatcag tagaaaagag
      481 agaagctgct gctgagcctt ggcattggtt gagattgaga tacggtgagc caatatacaa
      541 gagagaagac tctgaaagtg ttgagaaaag ggaggcaaat gctgacgcag atgcatggca
      601 ttggcttagg ttaagatatg gcgaaccaat atattaagga tcctgagacc agaccaataa
      661 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      721 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      781 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      841 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      901 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      961 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
     1021 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     1081 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     1141 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1201 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1261 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1321 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1381 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1441 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1501 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     1561 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1621 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1681 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1741 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1801 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1861 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1921 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1981 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2041 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2101 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2161 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2221 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattc
//
