LOCUS       sequence-351571-        2148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Kazachstania naganishii (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK012
FEATURES             Location/Qualifiers
     source          1..2148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(442..459)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     terminator      complement(523..557)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(628..1287)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1202..1221
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1469..2057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1958..1977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtag tcggtctcat atgaaatttt ctactatttt atctgctgct gctttagctg
       61 cttctgcttt tgctgctcca attgaacaac cagaacaaga tttgaattct actactattc
      121 cagctgaagc cattatttct tacttggatt tagaaggtga taaagatatt gctgttgttc
      181 cattttctaa tgctactgat tctggtttat tatttgttaa tactacaatt ttagctcaag
      241 ctaataaaga agctggtact ccattacaaa aaagagaagc agacgctgat gccaatgcct
      301 ggcactggtt gagattatct tatggccaac ctatttacaa aagggatgct aacgcagatg
      361 cagatgctga cgcagatgca tggcattggc taaggttaag ttatggtcaa ccaatataca
      421 agagagatgc aaatgcagat gctgatgctg atgctgatgc ttggcattgg ttaagattgt
      481 catatggtca accaatttat taaggatcct gagaccagac caataaaaaa cgcccggcgg
      541 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
      601 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
      661 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
      721 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
      781 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
      841 gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
      901 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
      961 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     1021 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     1081 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     1141 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     1201 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     1261 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     1321 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     1381 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2101 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtgg
//
