LOCUS       sequence-351572-        2802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Tetrapisispora phaffii (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK028
FEATURES             Location/Qualifiers
     source          1..2802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     544..563
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      811..1399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1300..1319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2667..2701)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(2772..2802,1..629))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
       61 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      121 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      181 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      241 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      301 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      361 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
      421 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      481 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      541 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      601 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
      661 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
      721 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
      781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      901 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
     1501 tcggtctcat atgtctcaaa ttccagaatt gagctcttat ttttatgatt ctacatacaa
     1561 tcctggtgaa tccttggtat catatacctc tatttatggt aatgacactg aagtttcttt
     1621 caatgagcta caaagcatcg ttaacaagag agttaatgaa tctattatgt ttggtgtcag
     1681 atgtggtgca gcttgtttaa tgtttattgt tatgtggatg atatctaaaa ataagaaaac
     1741 accactattt ataattaatc aatgttcatt ggtgttcatg attattcatt ctggtttgta
     1801 tttcaaatat ttattgtccg attacgctag tgttacttat tctctaactt actttcctca
     1861 attgatcaat agaagtgatg tccatttgta cggtgctgct tccatgttcc aagctttctt
     1921 agtaggttcc atcgaattaa gtctggtttt ccagattaag gttctatttc aatctgatac
     1981 gtataacaaa agactgggtc aagttgtttt gggtattggc gcggctttgg gtttaaccac
     2041 ttttgcaatg tacttggtca cagctatcaa aggtatggtc gctttatatg ctgatacatc
     2101 tgatatggaa caatattttt tcaacgtatc taccatttta tttgcttcat ctttgaattt
     2161 tttgacttgt ttgttggttt taaaattggc tttagttgtc agaacaagaa gatatttggg
     2221 tttgagacaa tttgattcgt ttcatgtttt gttaatcatg gcatttcaga ctttattaat
     2281 accatccata ctcttcatca tagcatattc tataaatgct tctaatgatg ttgatgaact
     2341 aattatcatt ggtggtctat tggtggtatt aagtttgcca ttgtcatcta tgtgggcttc
     2401 ttccattaat aattctgtta gacctacaag tttaaatact agtctttctc cacaaactac
     2461 atggtcttct gacgaaaaat ctatctattc agatcaaatg gagccagaca ttaaaactaa
     2521 aattacaaat aaagttggtg ggtttttacc atttgaaaag acaggaatgg tttcaactcc
     2581 aacagaatca actatttcaa tgcaaacaga tattgaaaaa gcttccacta ttgattattc
     2641 caagtgagga tcctgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
     2701 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     2761 tcgatatcaa attacgcccc gccctgccac tcatcgcagt ac
//
