LOCUS       sequence-351573-        2178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            alpha-factor gene of Tetrapisispora phaffii (sender part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK017
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(646..680)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(751..1410)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1325..1344
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1592..2178,1..2)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2081..2100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tagtcggtct catatgaaat
      121 tatcatcagt tttatctact ttagctttga ctgctacttc agcatttgct gctccagttt
      181 ctaatgaaag tgttgataat gcttctgttc cagctgaagc tattattggt tatttgaatt
      241 ttgatggtgc taatgatatt gctttattac cattttcaaa ttcaactact tctggtgtta
      301 tgtttattaa tacaactatt gcagaacaag cttatgaaga agctggtgtc tctttgtcta
      361 aaagagaagc agacgcctgg cactggctga gattggaccc aggccaacca ttgtacaaaa
      421 gggatgctga agctgatgca gaggcatggc attggctaag attagatcct ggacaacctt
      481 tgtataaaag ggacgcagaa gccgatgccg aagcttggca ttggttgaga ttggatccag
      541 gtcagccatt atacaagaga gatgcggaag cagatgctga agcttggcat tggttaagat
      601 tagatcctgg tcaaccttta tattaaggat cctgagacca gaccaataaa aaacgcccgg
      661 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
      721 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
      781 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      841 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
      901 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
      961 ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1021 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1081 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     1141 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     1201 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     1261 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     1321 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     1381 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     1441 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     1501 tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1921 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2161 gggggcggag cctatgga
//
