LOCUS       sequence-351575-        3426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Saccharomyces cerevisiae (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK034
FEATURES             Location/Qualifiers
     source          1..3426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1758..1792)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1863..2522)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2437..2456
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2704..3292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3193..3212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgaaactt attttggcga gtttcgcgat tattaacacc attactgctt taacaaacga
       61 tggcactggt cacttagaat tccttttaca acacgaagag gagatgtatt acgcaacaac
      121 cttagatata ggtacaccgt cccaaagtct gacagtgttg tttgataccg gatctgccga
      181 tttttgggtt atggattcta gcaatccctt ctgcttacca aattcaaata cgtcatccta
      241 ttcaaacgca acttataatg gcgaagaagt taagccttca attgattgca ggtctatgag
      301 tacttataat gagcatagat cttccaccta ccaatatctg gaaaatggta ggttttacat
      361 cacatatgct gacggaacat ttgctgacgg tagttggggg acggaaactg tatcaattaa
      421 tggaattgac atccccaata tccagttcgg agttgccaag tatgctacga cacccgttag
      481 tggtgttctt ggaattgggt ttcctagaag agagtccgtt aagggctatg aaggtgctcc
      541 taatgaatat tatcctaatt ttcctcagat tttaaaaagt gaaaaaataa tcgatgtggt
      601 cgcgtattcg ctgttcttaa actcacctga ttcaggtact ggttcgattg tttttggtgc
      661 cattgatgaa tcaaagtttt ctggtgattt gttcactttc cctatggtaa atgaatatcc
      721 cacaatagtc gacgctcctg caactttagc aatgactata caaggattag gtgcccaaaa
      781 caaaagtagt tgtgaacatg aaacgtttac gacgaccaag tatccagttt tgttggactc
      841 aggaacctcg ctattgaatg cgcccaaggt catagcagat aaaatggctt cttttgtaaa
      901 tgcgtcctat agtgaagagg aaggtatata tatattagac tgtccagtat ctgtaggtga
      961 cgtggaatac aattttgatt tcggcgattt gcaaataagt gttccactgt ctagtttgat
     1021 tttaagtccc gagacagaag gcagctattg tgggtttgcg gtccagccaa caaacgattc
     1081 gatggttctg ggtgatgtgt tcctgtcctc tgcatacgtc gtattcgatc tcgataatta
     1141 taagatatct ttagcacagg caaattggaa cgcaagcgaa gtttcgaaaa agctagtaaa
     1201 tattcaaaca gatgggtcta tttcaggtgc caaaattgct acagctgaac cctggtccac
     1261 caatgaacca tttacagtca cctctgacat ttattcatct acaggctgca agagtaggcc
     1321 ttttcttcaa tcatcgacag cctcttcgct tattgcagaa accaacgtac aaagtcgcaa
     1381 ctgctctacg aagatgccag gcactagatc aactactgtc ttaagtaagc ctactcaaaa
     1441 tagtgctatg catcaaagta caggcgctgt cacacaaacc tcaaatgaaa ctaaattaga
     1501 attatcctcg actatggcaa attcgggcag tgtctcgctt cccacttcga attcaataga
     1561 caaagagttc gaacattcga aatctcaaac taccagcgat ccaagtgtag cagagcattc
     1621 tacgtttaac caaacgtttg tacatgaaac taaatatcgg cctactcata agacagtcat
     1681 aacagaaact gtcacgaagt attctacagt cttaataaat gtctgtaaac caacatatgg
     1741 atcctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1801 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1861 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     1921 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1981 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     2041 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     2101 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     2161 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     2221 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     2281 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     2341 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     2401 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     2461 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     2521 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     2581 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     2641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2821 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2941 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3061 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3121 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3181 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3241 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3301 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3361 tgcgttatcc cctgattctg tggataaccg tagtcggtct catatgtctg caattaatca
     3421 tctttg
//
