LOCUS       sequence-351576-        3354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Kazachstania naganishii (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK033
FEATURES             Location/Qualifiers
     source          1..3354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1690..1724)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1795..2454)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2369..2388
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2636..3224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3125..3144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagttttgct ggctgccatt tccccattct tgtcaatttt ggttgaatgt tccccattgg
       61 ctttgccatc cactcatcaa aagttctcca cccagttggc aaagagaaat catttgaagt
      121 ttgatctcca atttgaggat gaaagctttt atgcaacgac attaaagatc ggtgacccac
      181 cacaagagat aaccgttatt tttgattcag gttctgctga cacatgggtt atgtctagat
      241 ataatccatt ttgtttgtcc agctctaaca ttacggcaaa tggactaaaa actctctatg
      301 atggcaaggc cattattccc aaagttgatt gttctcaaat taaaacctac aatgctaaca
      361 ggtctacgtc ttttcaagaa ttgaataatg ggcgttttta cattatttat ggagataaaa
      421 catttgctga tggtatttgg ggtaccgaaa cgttcgaatt tggtggtgtg aaaattccta
      481 acgttcaatt tggcctggca gattacgcta ctaccccaat tggcggcgtg ctgggtatag
      541 gtttccaaag aagggaatca gtcaaaggtt atacaaatgc accagaagta ttctatccaa
      601 attttcccca ggtattgaaa aatgaaggta tcatcgacac tgttgcttat tctctgtctt
      661 tgggtcaatc gtcacatatt atgtttgggg cgatcgatag aaactcatac atcggagata
      721 tggttacttt cccaatgatt aacatgttcc ctaatgctgt tagcaaacca gctaccctta
      781 gcatcaccat ccaggggctc ggtgctcaga acaagcaaga gtgcaaattt caaacttttt
      841 ctacaaccaa gtaccctgct ttgctcgatt ctgggtcgac gctgatttct gctccagtgg
      901 aaatcgcaga caaaatggct gctttcattg gggcccaatg gtctgaaagc gacggaatat
      961 atactttaga atgcccaaac gacacgacca atattgatta tattttcgat tttggtgata
     1021 tgaaattcac attgcctcta agcaacttga ttcttccatc ccaaggcggc agtaatgttt
     1081 gtggtttcgg tttgcaaagg agtgataatg agatcgtgtt gggcgatgtt tttttatgtt
     1141 cgactttggt agtgtttgac ttagatcatt atcaaatatc tctcgccaag agcaatcaaa
     1201 aaagttcagc agaagatatt gttaatatac caagtgacgg taagattcca ggtgccatca
     1261 ttgctaaggc tgaagcttgg accacctatg aaccaataac agccacaacc gacatgtttg
     1321 aaactgaaag atctcattgt ggcatcagaa gtgctacctc ttctgttgtt aactcagcta
     1381 aaagctcttt cactaatgtc aagtctgcaa cttgggaagt tgcctctaat acaacaatca
     1441 aatcttccgt tgagtcatcg tcaaaattat ttttgagcaa gggattttca tcaatgtcaa
     1501 actccaaaaa gacaggaatt tcgacacatc ctcaacatgt taagagcttg agagcttcta
     1561 gtagtattat ctcaacaaac acttcgcccg tcgaaatgag taaaataact attactacta
     1621 cgattagaga aacctctgta attacaagaa cagtttgcgg tactcaacca ggatcctgag
     1681 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1741 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1801 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1861 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1921 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1981 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     2041 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     2101 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     2161 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     2221 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     2281 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2341 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2401 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2461 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2521 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
     2581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2761 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3301 cccctgattc tgtggataac cgtagtcggt ctcatatgtt gttgttgtac gtcc
//
