LOCUS       sequence-351577-        3198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Kluyveromyces lactis (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK032
FEATURES             Location/Qualifiers
     source          1..3198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1592..1626)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1697..2356)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2271..2290
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2538..3126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3027..3046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatcccctg attctgtgga taaccgtagt cggtctcata tgattttacc ttctctcaca
       61 attctatgtt tactgacatc tttaataaac tgtcgtcaaa tagagttcga tcatgattca
      121 aaagtcattc agctgcaact acttaaacca gctgatagaa gtctacagaa aagatctaaa
      181 agttttgaaa gtaaactaaa tccacaggac tacttttata ccgttgaact tggtgtaggt
      241 acaccatcgc agaaaataaa ttgtatattc gatactggat cgtcagattt atgggtaccg
      301 gctaagagta atccattctg tctaggaaac aagctagata caaatggtac taatgaattc
      361 catggcacag aaataacacc aatactggat tgcgataaga ttggcgtttt cgacccaggt
      421 tcgtcctaca gtctgagtgc aactgggtat aatttctcca ctcagtactt tgacggcagc
      481 tattcagatg ggttttgggt cgtcgatgat atttcaattg ccgaccagca ggtcccagat
      541 ctgcaatttg cagttgctaa tatatcaagt gctccatcag gtgtactagg tgtgggactt
      601 cctagactgg aagcagttcg tggatacgat ggagccccca atgcgaatta cgataacttt
      661 ccgcagacac tgaaaagtaa tagattgatc aatagggtgt tgtactcgtt gtatttcgat
      721 agtggagatc tagaaagtgg aacagtgctc ttcggaggta tcgatgagaa aaaatacgat
      781 ggtgtgctgt atactttgcc attagtcaat atatatgaac agattgatga acccgctgct
      841 tttgatatca ctttacaagg acttggaata agaagtgaat cgaattgtaa agatactgtc
      901 atcgcgcata aaaaggcccc agcactactt gattctggta gtacaatcat gggagctcct
      961 caagaggcac tagaaagtat tgcccaagaa ctgggcgctg cattctcgga aagcgacggg
     1021 ttgtatgtgt tagattgccc agcatccaat gatgatcgca cattttattt cgatttcggt
     1081 gaattgaaac tgaaagtgcc aatacatgac ctcttatatc tacctgaatc cggtcagaat
     1141 tattgtgggt tggcaataat tgcgagtggc gaagagtgga tcctggggga tcttgttttg
     1201 aaaagcgcat atgtagtata tgaccttgat aacttggaga tctctatttc ccaggtaaaa
     1261 accacgagcg ggtcaagaat acaaacagta agtgcaacag gctctctgcc tcaaactaga
     1321 aaatcaaatt ctaccccctg gagtctgtct ggcagtacca actcaagtac agactctgta
     1381 tttgataagc ctttacctaa gaaatgtcaa aaatcaccct ctaagacatg tccggcaaag
     1441 aaaaaaggaa ataaaagaga agtcttaatc aatatacatg acaatctaat tatgcttgaa
     1501 aactctggtt cttctagttt aagaccatcc tatctgcttt gcgcattgtt agtactcttc
     1561 cattctctcc taggatcctg agaccagacc aataaaaaac gcccggcggc aaccgagcgt
     1621 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     1681 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     1741 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     1801 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     1861 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1921 aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     1981 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     2041 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     2101 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     2161 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     2221 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     2281 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     2341 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     2401 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
     2461 atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3181 cacatgttct ttcctgcg
//
