LOCUS       sequence-351578-        3219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Eremothecium cymbalariae (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK031
FEATURES             Location/Qualifiers
     source          1..3219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1499..1533)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1604..2263)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2178..2197
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2445..3033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2934..2953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catcctcaag aatgggatga tacttccaat gatggtgaaa aatgtttttc catggattta
       61 atcaacaata atgaccatta tttggcagaa attgaagttg gtaccccacc tcaaaagctg
      121 aaggttctgt tcgacaccgg ttctgccgat ttgtggtttc agtcctcgga taatgaatat
      181 tgtgctgcta gttaccccca aggtgacaat tctaccaaca cgaacgataa cgaacaaaac
      241 atggcttcct tttgttcatc ttcgagaacg tacaattttc aaagatcaag cactttcact
      301 cctaccggct ataaattttt tattgattat gtaaacacat tttctagagg tatatgggca
      361 aaagatagat tggtcattgg taacgcagat gtttctggtt tgcaattcgg cgtttctaac
      421 atctcgaata cgactattaa tggtattttg ggtgtcggat atgaaagatt agaatccgtt
      481 aagggttata gcggcgcacc caacaggaca tatccaaatc taccaatgct attgaaagaa
      541 tcaggtaaaa tcgataaagt tgcttattca gtctttttaa atcatctgaa ctctaatgtc
      601 tcgacggtga cttttggcgg cgtcgactca gaaaagtaca ccggcgactt atacaagttt
      661 ccgttggtga atatttacaa atctatccca agaccatcca agttccatat aactttacaa
      721 gcactggagg ttcatggtaa ttattcctcc ttgaacaaaa ctgtttcaaa tactccattg
      781 cctgctttgt ttgatactgg aactaacggt atggttgttc cacctaatgt tgccgaaaat
      841 atcgcatcca caatacatgc gacctttgat gaaaagttct ctttgtatct atttgcctgt
      901 ccagctgaag aagatgacac aaaattggtc ttcgattttg gtgatatcca aatcgaggcc
      961 ccactcgcca actttatagt tcctgcagcc gaattaggaa cagaacaatg tggggtcgcc
     1021 attatacctg aggatggtac attcaccctg ggaataccat ttttgagatc cacctacgtt
     1081 gtgtacaact tggaagacta cgaagtagcc cttgctcaag ctaattggaa atgggacgga
     1141 aagtctaaaa ttacacctat cattagtaac attcccggcg ctacacaagc cactgctacg
     1201 ccatggaaca cgaatatgga acagtcgact gattcaatca gctctacgtt tggtatcaca
     1261 gatatttcac cttcgacaat gtacccaaca ggcactcaaa caacaaccat ctctgatcat
     1321 atcactaaat atacagtaga accactgcac tctcctaaca ctttgacctt cacaaatata
     1381 tctccaacac tcacttctca agtttccacc gtattcattg ttactactac cgttaccgcg
     1441 accactacaa ttactttgtc tcattgtcaa aaaagaattg gatcctgaga ccagaccaat
     1501 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1561 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1621 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1681 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1741 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1801 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
     1861 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1921 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1981 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     2041 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     2101 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     2161 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     2221 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2281 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2341 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     2401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3121 gtggataacc gtagtcggtc tcatatgctg ctagttattc attctatgtt attcatcacg
     3181 cttttgttgt tcccaaagtt ggttttcggt ggcttgtta
//
