LOCUS       sequence-351580-        2970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Bar1 gene of Candida albicans (barrier part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK030
FEATURES             Location/Qualifiers
     source          1..2970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1737..1771)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1842..2501)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2416..2435
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2683..2970,1..301)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
       61 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt agtcggtctc atatgttgtt
      421 tactatattg tctttgttag ttccttctct agctgtcgct gtctccaata ctggtggtgc
      481 tgtaaaatta gatctgacaa tatctgatga acatatgtat tatatcgaaa atttggcttt
      541 aggtacacca ccacaattta tttcgaacgt tattgtggat tctggttcat cggatttgat
      601 gattgtagat tccatttata acttctccgc ttcttcatct ctttacaatt caaatcaaac
      661 tgccattatg aaatacggct atggtggcca attcccagtc tacttcatta atgaaactat
      721 tagatcaaat gattggaaat taagtaattt atctgtgggt ttagctaaca tttcagatat
      781 ggattcattc agtggtatcc tcggtattgg tttcacaaga caagaactat tcaagactaa
      841 ctacagtaac tttccatatt tattaaaaga tcaaggttat accaagtccg ttttatttag
      901 tttcaatggt caagaccaaa atccatctat tattttcgga ggtataagca cgaatataat
      961 tgatggtcca ttggttagag ctccattcat caaagttatc tcatttatca accaattgga
     1021 ttactggtta atgccaactt ttacagttaa tcaaattaaa ttgggtgata ccattgtttc
     1081 taaccaaaaa acattatatc aaatcgatag tggtactaat ggttttgttc caccaactcc
     1141 agtcctaaat aatattttga aaattttagg tgacgattat attcaagatg acaacgggaa
     1201 tatctatttt gatatcaagt acatagaagg tttgaatatt acattttctg tgcaaggata
     1261 tgatattgga tttcaacttg ttgatatagt tggtgataca attgaaagaa actcaactac
     1321 tttcgtggca ttaaatgtgg cttcatgtga tatcggttat aatgcttatg aaggattatt
     1381 gccaaatttg attttcaaat atcattatgc aatttttgac tacgataatg cacaaatata
     1441 ctttggtaaa tacaaaaatt ctaacggtga agctaatgtt gttgcagttg agaatggata
     1501 ccaacttcca gtaccaaccg tcgatgtccc agatgttgaa gatacttatt ctgtcattta
     1561 cattgctgaa tctgaaacaa ctgttaccgt tgctccaaca gaagttcaat ctacttactc
     1621 atccttttca atttccgaag gtacctctag caacttgact tccggttcga catctgatga
     1681 taaaataact tctgttactt ctaatcctca atattgtgga tcctgagacc agaccaataa
     1741 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     1801 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     1861 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     1921 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     1981 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     2041 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
     2101 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     2161 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     2221 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     2281 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     2341 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     2401 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     2461 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     2521 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     2581 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     2641 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2701 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2761 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2821 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2881 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2941 gtgtcttacc gggttggact caagacgata
//
