LOCUS       sequence-351581-        2820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Vanderwaltozyma polyspora (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK029
FEATURES             Location/Qualifiers
     source          1..2820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(32..66)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(137..796)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     711..730
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      978..1566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1467..1486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agaacttctt gaggatcctg agaccagacc aataaaaaac gcccggcggc aaccgagcgt
       61 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
      121 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      181 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      241 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      301 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      361 aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      421 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      481 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      541 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
      601 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
      661 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      721 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      781 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
      841 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
      901 atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1621 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg gtctcatatg
     1681 agttcacaat ctcatccacc attgatcgat ttattttacg attcatcgta tgaccctggt
     1741 gaaagtttga tatattacac ctcgatttat ggtaacaaca cttatattac ttttgatgaa
     1801 ttacaaacta ttgttaataa gaaagttact caaggtattt tgtttggtgt tagatgtggt
     1861 gctgcatttt tgatgttggt agcaatgtgg ttgatttcta aaaataaaag atctagaatt
     1921 ttcatcacca accaatgttg tttggttttt atgattatgc acagtggatt gtattttaga
     1981 tacctattat caaggtatgg gtctgtcacc ttcattttga ctggttttca acaactactt
     2041 acaagaaatg atatccatat ctacggtgcc accgacttta ttcaagttgc attagttgct
     2101 tgtattgagt tgagtctgat attccaaatt aaggttattt ttgctggtac caattatgga
     2161 aaactagcta attactttat cactttgggc agcttattgg gtttagctac attcggtatg
     2221 tatatgctta ctgcaataaa tggcaccatt aaactgtata acaatgaata tgatccaaat
     2281 caaagaaaat acttcaatat atctacaatc ctacttgctt cttctatcaa tatgttaact
     2341 ttaattctta ttttgaaatt ggtggctgct atcagaacaa ggagatattt aggtttgaag
     2401 caatttgact cttttcatat tttattaata atgtcaacac aaacattaat cattccaagc
     2461 atattattta ttttgtctta ttctttgaga gaagatatgc atactgatca attaataatt
     2521 attggtaatt tgattgtcgt tttatctttg ccattaagtt ctatgtgggc tagctctttg
     2581 aataattcat caaaaccaac atctttaaat acagattttt ctggtccaaa atctagtgaa
     2641 gaaggtactg ctattagttt attatctcaa aacatggaac catctattgt tacaaaatat
     2701 actagaagat ctccaggttt atatccagtt tctgttggta caccaattga aaaagaagct
     2761 tcatatacat tgtttgaagc aactgacatt gatttcgaat cttcatctaa cgatattacc
//
