LOCUS       sequence-351584-        2994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Lachancea thermotolerans (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK026
FEATURES             Location/Qualifiers
     source          1..2994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     336..355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1895..1929)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2000..2659)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2574..2593
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2841..2994,1..435)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
       61 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      241 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      421 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      481 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      541 tctcatatgg gttctgaatt ctctccatcc actgccaaca ccacaatggc tactttaaga
      601 tccgcctccg aaatgttgct gacttttgaa tccgcattct ccaatgatac acaagtagat
      661 tttgggcaat tacaagcatt ggctgacacc agaatcacgc aaggtatttt attcggtact
      721 aggattggta cctcggtctt gaccattatt accctgtaca tggtttcaaa gaatagaaag
      781 actccaattt ttatttctaa ctttgtctcc ttgtttttgg ttgccttgca ctccgccttg
      841 tacctgggtt acttgacgac tccatttgcc tccttgactt tcaatttgac tttattccca
      901 gatttaattc caagatcaaa tttacatttg tttgccgtaa caaatgttgt tcaagtttta
      961 ttggtagctg ctgttgaagc atctttaatt ttacaagtta ggattatttt ccactctaat
     1021 acattaaaac ctgttggtag agttcttgtt gcattgagtt ctgttcttgc agctgcaact
     1081 actgttttgt tttttgtttc cgctgttaag tctattagaa gcgtttatgc agatgtttta
     1141 gctatccaag atactttgtt ctttaatttg gcaacaataa tgttggccac ttcagtaaat
     1201 tttatgactt tgttattgac agttaaattg gttttggcaa ttagatcaag aagattttta
     1261 ggtttgaaac aatttgactc ttttcatatt ttattaatta tgtcttttca aacattagtt
     1321 tttccctctt tattattgat tttgtcctac gctttgaatg gtaagaaagg tacaaatact
     1381 ttgtcaactc ttgctacctt attagttact ttgtctttgc cattatcttc tatgtgggca
     1441 acatctgcca ataattcacc tagaccatca tcagtgaata caagattttc tccatgtttg
     1501 tctgataatg gttctagtaa atttgataat cattctctat tttctcattc aatgaattct
     1561 actgcttatc aaagacatga agatagagca tatgatttat atccaatttc tagatctggt
     1621 aatattgatg aggattatgg ttacggtatg gaaccattgg ataaagataa ttgtaggact
     1681 ttggcagttt ctgtcaaatc aggtgataca tctccaggtt cttcttcaca tggtgaacta
     1741 tctagattgc catcatctga tgctaatcaa caaactttag aaactttgga aagagctatt
     1801 caagctggtt ctttcactcc aaatacagtt gatgatgaag ctgctagaag tttttgggct
     1861 aacgctaaac catgaggatc ctgagaccag accaataaaa aacgcccggc ggcaaccgag
     1921 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1981 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     2041 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     2101 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     2161 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     2221 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     2281 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     2341 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     2401 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     2461 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     2521 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     2581 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     2641 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     2701 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     2761 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tcta
//
