LOCUS       sequence-351585-        2970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Lachancea mirantina (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK025
FEATURES             Location/Qualifiers
     source          1..2970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1334..1368)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1439..2098)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2013..2032
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2280..2868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2769..2788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggtctcata tgttgaacta tccaaatagt gaaatgattt ttaatgcttc tcaacaaaca
       61 ttgtcatact cttcccttta cggcaatgac actaccattt cattttctga tttaaatgat
      121 attgttgcta ccagacaaag cgaaagtatt atttctggag ctagattgag tgctgctggt
      181 ctagctctca tcattctgtg gatggttagt caaagaagaa ctactccaat ctttattgct
      241 aatcaagttt ctttgacttt aatggtcatt caatcttcgt tgttgattgc ctattcttat
      301 acacattatt catctatcac tttcactcta acaatgttcc ctcaatatgt tccaaggtcc
      361 gatctttatt tttttggtgc atcaaatatt ttccaagctt tgctcgttgc agctgttgaa
      421 gttagcttag ttttgcaagt gagagtggtt tttaaatgtg ataacttcaa aaatgccggt
      481 agagctttaa ctgctatgtc cgctgccttg gccggtgcta caactggtat gtttttcgcc
      541 accgctatcc aatcaacaat cgctgctcat gaaaatgttt cctcagttcc tcctactatt
      601 ttatataatg cagccgttat tttgatggct tgttctgtta attttatgac cttgcttttg
      661 gcaattaaat taatattggc tataagatcc aggagatttt tgggtttaaa acaatttgat
      721 tctttgcata tcttattaat tatggcattt caaactttgg ttttcccaag cattttgttt
      781 atcctatctt actcgttgaa tggcaaatct ggcacaggtg acttggaagc catcgcgatt
      841 ctgcttgtta cattgtcatt acctttgtct tctatgtggg ctacttcagc caataattcc
      901 aacaaaccaa ctagtgtaaa cactcaattc tccccaagac catacacatc ttctagttat
      961 ggatcagaag atatgacagt ttattctaga aatatgaatt catgttcctc tggttctgaa
     1021 aagaataaat tgtatgactt gtacccagtt gaaggtaaag gtaatcattg tggtaactct
     1081 gcaggtgatg gttatttggg aaaagataat tatgattatt ctgctcaatc tattggttca
     1141 gcacaaaatt cttcaatgac tgaaaatgaa acaagaaacc atttgaatcc atcaagatat
     1201 ggtattagcg acaatttgag agatatgaac attgcatcta atgctactgt taaaacacca
     1261 gataccttgg atgatgaaga agcaagaaaa ttttggtctt tagctgaaaa ctgaggatcc
     1321 tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1381 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1441 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1501 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1561 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1621 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct
     1681 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1741 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1801 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1861 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     1921 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1981 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2041 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     2101 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     2161 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct
     2221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2401 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2941 ttatcccctg attctgtgga taaccgtagt
//
