LOCUS       sequence-351586-        3009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Lachancea fermentati (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK024
FEATURES             Location/Qualifiers
     source          1..3009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..32
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1587..1621)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1692..2351)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2266..2285
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2533..3009,1..112)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
       61 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      181 tgcgttatcc cctgattctg tggataaccg tagtcggtct catatgactt ttgatccaag
      241 tctaagcttt attacttata catctgcata tggttctgac acacagttat cttttgaaca
      301 attggaatct ttggctcaaa ccagactgac acaaggtatt ctttttggta ctaggatcgg
      361 tgctgctggt ttggctatta ttgtcttgtg gatgattaca aaaaacagaa aaacaccaat
      421 atttgttatt aaccaagtct cattgtcttt cttgtttctt cattcagttt tatatgttgg
      481 ttacttattc agacattatg cttcaattat ttacaattta actatgttta ctgattttgt
      541 tccaagatct gatgtctatt tgtacggtgc tataaatatt ttccaagttt tacttgtgat
      601 ttccattgaa gtttccttaa cataccaaat tagagtgatt tttaaatcag ataattataa
      661 aagaattggt agagttttaa tggtcatttc aattgttttg gccgttgtca ccgctgttat
      721 gtacttggtt acagccatca aatccatgat caccatatac tcagatatat ccggaaatcc
      781 tgatacagta ttgttcaatg tttctacaat attactatct tgttcggtca attttatgac
      841 attaattctc acatcgaaat tgttgctggc tatcagatcc agaagatttt taggtttgaa
      901 gcaattcgat tcatttcata ttttgatgat tatgtcttgc caaacactga ttttaccttc
      961 aatctttttt atcttggctt acgctttaaa taaaaatcag ggaactgaca ctttagcttc
     1021 tgtagcaact ttgttagtga cactatcttt accattgagt tctatgtggg ctactgctgc
     1081 aaataactcc tcttacccaa cttctgttaa tactcaatat tcctcttcaa tgtatgatac
     1141 tgaaagtgat agattcgaaa aaccttctct ttatactcat tctgctaagt cttcagttaa
     1201 cttcaaaaaa tatcaaaatc atctgtacga tatttatcca gttactagag atcaaatggt
     1261 tgatcatggt tgtaatgatg ctgatttaga aaagaatcaa acaagaggta ctaatttgtc
     1321 aataagagat ggtgaatcag ctccaggtcg tggtgatagg tcggacgttg gaagattaga
     1381 tgttcctggt tatgatttga acgatgcgtc ttctgcctca aaagatatga taaatttgaa
     1441 tgtatacact ccaaatacat tggatgatga agaagctaga gaattttgga ctattaatga
     1501 ttcctcatat catccaagat tgacaaaggg ctgttctaag aaatactctg gttctatcaa
     1561 ttcttgagga tcctgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
     1621 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     1681 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     1741 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     1801 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1861 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
     1921 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1981 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     2041 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     2101 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     2161 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     2221 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     2281 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     2341 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     2401 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     2461 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2641 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3001 gcgcacgag
//
