LOCUS       sequence-351587-        3021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Kazachstania naganishii (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK023
FEATURES             Location/Qualifiers
     source          1..3021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1445..1479)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1550..2209)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2124..2143
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2391..2979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2880..2899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt
       61 cggtctcata tggattccga tggattgcca ttgcaaccaa tcagtcttgc attcgatcca
      121 acttacaata tcctaaattc aactctgggt tacttgacaa tttatggtaa cgacactgca
      181 acattttacg aagttcaatc tatcattaac aaaactgcta gagaatcaat tgcatatgga
      241 gtttgttgtg gtgcagttat tgttactttt gctgtcatgt ggttagtgtc tgacaagaga
      301 aagaccccaa tttttatctt aaatcaaatt agtttgttat ttacctttat tcaatcagca
      361 ttatatttta aatatttgtt ttcttattcc aattccctgg cttatagttt aacgggtttc
      421 tctcaaaaat taacaaatta cgataggcat ataaccgctg ctgcagattt gttcaaagtt
      481 tttttggtgg cttcaataga gctaagcctt gttttccaag tgagagttat gtttagtggt
      541 gactcattca agaaagttgg ttattcactg ttcttgctat ctgtaggcat tggtcttaca
      601 acagtagctg tttatctagc ttctgttatc atgattataa tagaattgta tgataatcag
      661 ttgattagag gtccaagatt ttataatatt gctaacattt tatttgccag ctctattaat
      721 tttatgactt tcatattagt tgtcaagctt tttttggcca tcagatcaag aagattctta
      781 ggtttgaagc aatttgattc atttcatatt ttactgatta tgtcaggtca ctctttaatt
      841 attccatcaa ttttgactat tttagcatat gctttatcca cttcaggaac agatgtgcta
      901 gttacagttt ctacattgtt ggttgttttg tcattgccgc tatcctcaat ttgggctcat
      961 tcttctaata cagctagtaa tgcaagtacc ttgggtaatg attttaactc cacggcgtcg
     1021 ggtttttatc caaatagttc tagatcatat ttaactcaat ccttgaaatc aacacctacc
     1081 actgccaaag agtctatgaa ggattatcaa actagattca gacatttata tagaaaagaa
     1141 aagacaggtg atattgaaga agaatattct ataaactcaa aacatagtga tactgattct
     1201 tcagaagtgc ctaaagatcc tttctatgaa atttcctcct ctttgtcgaa ggaaaaatct
     1261 gataaaagaa atactactaa gggtttagtt aaaaaattgg aattacaaga atctggtgaa
     1321 gtagatggtt cttttagatt atctactcca aacactgaag ctgatatgga agcaagaaaa
     1381 ttttggttgg atgaatctcc tgtttcagat caaacaagaa tttgaggatc ctgagaccag
     1441 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1501 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1561 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1621 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1681 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1741 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
     1801 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1861 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1921 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1981 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     2041 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     2101 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2161 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2221 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     2281 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     2341 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2401 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2461 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2521 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2581 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2641 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2701 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2761 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2821 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2881 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2941 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3001 ttacggttcc tggccttttg c
//
