LOCUS       sequence-351589-        2976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Kluyveromyces lactis (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK022
FEATURES             Location/Qualifiers
     source          1..2976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1363..1397)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1468..2127)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2042..2061
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2309..2897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2798..2817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgattctgt ggataaccgt agtcggtctc atatgtcaga agagataccc agtttgaacc
       61 cattgttcta caatgagaca tataatccat tgcagtccgt cctaacatac agttcaattt
      121 acggagatgg gactgaaata acatttcaac agctacaaaa tcttgtccat gaaaacatca
      181 cccaagcaat tatttttgga acaaggatcg gcgctgctgg attagcgttg attataatgt
      241 ggatggtatc taagaataga aagacgccga tattcataat aaatcagagt tctttggttc
      301 ttacaattgt tcaatctgct ttatatctat catatttgtt gagcaatttt ggaggagttc
      361 cctttgctct aactttgttc ccacagatga taggcgaccg tgacaaacat ctttacggtg
      421 ccgtgactct aattcaatgt ctattggttg cgtgtattga ggtttcatta gtctttcagg
      481 taagagtcat tttcaaagca gatagatata ggaagatagg aatcattttg actggcgtat
      541 ccgctagttt tggtgctgca actgtagcca tgtggatgat tactgcaata aaatctatta
      601 ttgtagtgta tgatagtcca ttgaacaaag ttgacacata ttattacaac atagcagtta
      661 ttttacttgc atgttcaata aatttcatca ctcttcttct atcagtgaaa cttttcctgg
      721 ctttcagagc taggagacat ttaggtttga aacaatttga ctcatttcac attctactca
      781 tcatgtctac tcagacatta ataggtccat cggttttgta tattctcgcc tacgcgctga
      841 acaataaagg agttaagtcg ttgacttcta ttgctacatt gcttgtagtt ctttccctac
      901 ctttgacatc tatctgggct gctgctgcaa atgatgcacc aagtgccagt actttctatc
      961 gccaattcaa cccttactct gcacaaaatc gtgatgattc atcatcctac tcttatggta
     1021 aagcctttag tgacaaatac tctttcagta actcaccaca aacttcggat ggttgtagtt
     1081 caaaggaact tgaactatct acacagttgg agatggattt agagtctggc gaatctttta
     1141 tggatagagc aaaaaggtcc gattttgttt cttctccagg atcaacagat gcaacagtga
     1201 ttaaacaatt gaaagcttcc aacatctata cctcagaaac agatgctgat gaagaggcaa
     1261 gggcattttg ggtgaatgca attcatgaaa acaaagatga cggtttaatg caatcgaaaa
     1321 ccgtattcaa agaattaaga taaggatcct gagaccagac caataaaaaa cgcccggcgg
     1381 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     1441 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     1501 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     1561 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1621 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1681 gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1741 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     1801 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     1861 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     1921 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     1981 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2041 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2101 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2161 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2221 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2941 ggccttttgc tcacatgttc tttcctgcgt tatccc
//
