LOCUS       sequence-351590-        2934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Kazachstania africana (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK021
FEATURES             Location/Qualifiers
     source          1..2934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1211..1245)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1316..1975)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1890..1909
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2157..2745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2646..2665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acttcaattt atggtaacga tactgccatc accttcgatt atttaaatga tattgtgaat
       61 gagaaaatta gagaaggtat tttatttggt gttagatgtg gttgtgctac attgactaca
      121 attgttatgt ggttggtttc taaaaagaaa aaaactccaa tttttattat aaatcaaatt
      181 tcattgattt tgattattat tcatagcggc ttatatttta agtatttgtt ttctaatagt
      241 acttctgtta catattattt gactttgttt cctcaattag tctctagata tgacttgaat
      301 atttccaatg ctgcatctat gtttgacgtt ttgttagtcg cttgtattga atgttcattg
      361 gtttttcaaa tcagaattat gtttacaggt gctaataata acattcaaaa atattcttta
      421 atgttgattg ctttatcaac cttattggga atctctactg ttggtatgct attaacttat
      481 gctattaagt ctatcatatc atcatacaat ggttcttatg ttaatccaaa atactataat
      541 attggtttca ttttattctc gtcatcaatc aatttcatga ctttcacctt agttgttaaa
      601 ctattggtag caataagatc aagaagatac ttgggtttaa aacaatttga ttcatttcat
      661 atactgttaa tcatgacatg tcaaagtttg ttagttcctt caataatagt tattctagca
      721 tattccctga gttctacaga atcaactgat gttttaatta caatttcaac tttattggtt
      781 gtattatcat tacccttatc tagtatgtgg gcttctgcta acaataatat gtctactact
      841 accatttcaa attcttacct ttctgactat acacaatcag atggtactgg tttccatcca
      901 aacgacggtg attctaacca ttcattccag ataagatctt caccaagaag tgaagttacc
      961 aaaatagatg atttggaaaa aaacgcattt tatgaattaa caagaactaa atggcaaaag
     1021 tcaaactctg attcctcaga tttagatgac tccagtttag cagacgattt atacactcca
     1081 aatactgaag ctgataaaga tgctagaaat ttttggatta acgaaaatga aactatagaa
     1141 aacattaatg cagatacaaa ttcatctggt ttgaatgacc ctaatccttg aggatcctga
     1201 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1261 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1321 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1381 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1441 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1501 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     1561 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1621 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1681 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1741 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1801 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1861 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1921 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1981 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2041 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     2101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2821 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgt caatggctga ctacaacatc
     2881 agttcacttg tctacgatcc ttcgtatgat ccaactcaat ccattttatt atat
//
