LOCUS       sequence-351591-        3075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Eremothecium cymbalariae (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK020
FEATURES             Location/Qualifiers
     source          1..3075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     173..192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1813..1847)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1918..2577)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2492..2511
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2759..3075,1..272)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
       61 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      361 tggataaccg tagtcggtct catatgtcag aggatactct aattccaatt gagcagtatt
      421 acgatccatc ttacaatcca agagcttcat tattaactta tgaatcaatt tataatcata
      481 aaacaacagt ctctttcaaa gatatcgaag agtttattaa taccaaaatc acacaaggtg
      541 tttttgttgg tactaaaatt ggtgctgctg gtttggctat tcttgttctt tggatggttt
      601 caaaaaatag aaaaactcca atttttttaa ttaaccaagt ttcattgttc ttgactttga
      661 cacattcttc gttgtatttg aaaaatttat taggtcattt ctcaagtgta acttttgcat
      721 ttaccttatt cccacaatat atttcaaata atgatttaca tgtttatgct gccaccaata
      781 tgtttcaagt cttcttagtt gccgcaatag aagtaagcct tatctttcag gttagagtca
      841 tatttagagc agataactac aagagagtag gcacaatact gacatccatc tctacagttc
      901 tggggattac cactgttgtt ttatacttgg ttactgccat caattcaatt aagaatgttt
      961 actacgatgt ttctaagacg tccgatgctt attatttcaa catttctact atattgatgg
     1021 catgttctgt taattttatg actcttttac taacagtaaa attgattttg gcgataagat
     1081 caagaagatt tttgggttta aaacaatttg attcatttca tattttgtta ataatgtcta
     1141 ctcaaacttt gttatgtcca tctgttttgt acattttagc atatgcttta agttctagag
     1201 gtacagattc tttaacaact attgctactt tgttggtcac tctatcttta ccattgtcat
     1261 caatgtgggc tacatctgct aataactcta caaaaccatc gagtattaat acccaattct
     1321 ctccatataa ctgtgaaact atgtcttttg gaactggttc ttcaacttac tatgcaaatt
     1381 ctatgaatag taataaaaga tcaataacta ataagttgta tgatttatat ccagcaaacc
     1441 atttttcttg taaaagatca tatggttctg gagaagataa tactattgtt gatattgaaa
     1501 aaaaatgttt taatatcggt tcttcaattg aaagttcacc agctcaacca tcaacgttga
     1561 acaatggcac ttcctctcat aataaatcag taaatggtaa cgttgaagtt caaacaccaa
     1621 ctaccgcagc agaagaagac gctaaaaggt tttggactga tacatattat cctactggta
     1681 ttcctgatga agaatttaca gattttaaag tttctggtga acaatgtaat attttccaat
     1741 ccccgacatc aaagactaga aacaaatctt attctagaaa tacaattaga tgaggatcct
     1801 gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     1861 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     1921 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1981 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2041 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2101 gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc
     2161 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2221 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     2281 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2341 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2401 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2461 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2521 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2581 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     2641 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt
     2701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2881 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3061 cgcagcggtc gggct
//
